Nucleic acid amplification

a technology of nucleic acid and amplification, applied in the field of nucleic acid amplification, can solve the problems of high cost or insufficient coverage of wga methods, limiting the dna yield of human samples, and insufficient average dna size, etc., and achieves less complicated amplification and more consistent output
US20080128298A1Inactive Publication Date: 2008-06-05QIAGEN GMBH
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
QIAGEN GMBH
Publication Date
2008-06-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and / or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and / or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and / or concentration of nucleic acid, such below-threshold amounts and / or concentrations can be used in amplification reactions.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a divisional of co-pending application Ser. No. 10 / 429,229, filed May 2, 2003, which is a continuation-in-part of application Ser. No. 10 / 272,465, now issued U.S. Pat. No. 7,074,600, filed Oct. 15, 2002, which is a continuation-in-part of copending application Ser. No. 09 / 982,212, now issued U.S. Pat. No. 6,617,137, filed Oct. 18, 2001, which is a continuation of copending application Ser. No. 09 / 977,868, now issued U.S. Pat. No. 6,977,148, filed Oct. 15, 2001, each of which applications and issued patents are hereby incorporated herein by reference in their entirety.FIELD OF THE INVENTION

[0002] The disclosed invention is generally in the field of nucleic acid amplification.BACKGROUND OF THE INVENTION

[0003] A number of methods have been developed for exponential amplification of nucleic acids. These include the polymerase chain reaction (PCR), ligase chain reaction (LCR), self-sustained sequence replication (3SR), nuclei...

Claims

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