Composition Comprising Carbohydrates and Peptides which Comprise Tryptophan

Inactive Publication Date: 2009-10-29
CONOPCO INC D B A UNILEVER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]Hence, there was a need for a composition providing both tryptophan in a form which allows good uptake in the brain, which is from an industrially-widely available source, has a high tryptophan/LNAA ratio, a low al

Problems solved by technology

However, tryptophan is not the only amino acid taken up, and in fact when an average animal protein composition is ingested, the level of tryptophan taken up by the brain is so low due to competitive uptake of other amino acids that usually no significant effect can be observed attributable to tryptophan.
Use of tryptophan as free amino acids has disadvantages, in that food legislation in many countries limits the use of tryptophan as free amino acid in foodstuffs.
Due to the fact that tryptophan is present in relatively large peptides, the tryptophan uptake into the blood will be retarded hereby limiting the application possibilities of the preparation as a food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0085]Hydrolysing Lysozyme Using Protex and Identity of the Peptides Formed.

[0086]A solution containing 10% (w / w) pure lysozyme was adjusted to pH 8.2 using NaOH and heated to 52 degrees C. Hydrolysis was started by adding 25 microliter of Protex / g of protein present. Under continuous stirring and maintaining the pH at 8.2, the hydrolysis was continued for 5.5 hours to yield an almost clear solution without a visible precipitate. After a heating step to inactivate the Protex activity, a sample was taken for DH analysis. The DH of the solution turned out to be almost 30%. The heat treated solution was ultrafiltered over a 10 kDa filter to yield a completely clear liquid. This clear liquid was used for LC / MS analysis, for molecular weight distribution of peptides and proteins present as well as for ion exchange chromatography.

[0087]To get an impression of the molecular weight distribution of peptides and proteins present, the clear liquid was subjected to a molecular size analysis as ...

example 2

[0090]Increasing the Tryptophan Content of the Hydrolysate.

[0091]Lysozyme incorporates a surprising high amount of the basic arginine and lysine residues. Furthermore the lysozyme molecule incorporates a significant number of the acid glutamate and aspartate residues. This data has been used to devise an innovative and elegant purification route towards hydrolysates featuring high tryptophan / LNAA ratios. Essential requirement for this purification route is, however, that only very few of the tryptophan residues show up in peptides also containing either an arginine or lysine residue or a glutamate or aspartate residue. As shown in Example 1, the specific hydrolysis route used here yields only few trytophan containing peptides containing an arginine residue and no peptides containing a lysine, glutamate or aspartate residue.

[0092]Theory predicts that a maximal charge difference between peptides with and without a glutamate or aspartate residue can be achieved around pH 3. A maximal c...

example 3

[0094]Large Scale Lysozyme Hydrolysis.

[0095]In larger scale lysozyme hydrolysis procedures, essentially the procedure as described in Example 1 was followed with some minor modifications. A solution containing 7.3% (w / w) pure lysozyme was heated to 65 degrees C. after which the pH was adjusted to pH 8.2 using NaOH. Hydrolysis was started by adding 25 microliter of Protex 6 L / g dry matter. Under continuous stirring and maintaining the pH at 8.2 and the temperature at 53 degrees C., the hydrolysis was continued for 2 hours. Then the pH value was increased to 9.0 and incubation was pursued for another 3.5 hours to yield a solution with some precipitate. Then the pH of the solution was lowered to 4.5 and the solution was cooled to below 4 degrees C. To obtain a completely clear product, the liquid was filtered over a Z 2000 filter (Pall) and subsequently excess water and salt was removed via nanofiltration. The resulting concentrate was then subjected to an UHT treatment of 7 seconds at...

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Abstract

An edible composition comprising peptides rich in tryptophan, which edible composition further comprises a rapidly available glucose composition and a slowly available glucose composition.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a formulation comprising tryptophan-containing peptides together with rapidly available glucose and slowly available glucose (i.e. “fast” and “slow” carbohydrates).BACKGROUND OF THE INVENTION[0002]Recently, there have been various reports on the benefit which tryptophan is believed to have on several aspects of human behaviour, mood, brain function, brain development, when such tryptophan is taken up by the brain. Examples of such reports are WO 99 / 55174, WO 00 / 42868, WO 20051023017.[0003]Tryptophan is an amino acid present in many proteins, like e.g. whey proteins, but also animal protein contains tryptophan. Tryptophan can be taken up in the blood, and from the blood into the brain, after ingestion of a protein which contains tryptophan. However, tryptophan is not the only amino acid taken up, and in fact when an average animal protein composition is ingested, the level of tryptophan taken up by the brain is so low due t...

Claims

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Application Information

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IPC IPC(8): A61K38/06A61K38/05A23L27/30A23L29/269
CPCA23J3/34A23L1/0548A23L1/09A23L1/095A23L1/2363A23L1/2364A23V2002/00A23L2/52A23L1/3053A23V2200/322A23V2250/065A23L5/00A23L27/33A23L27/34A23L29/274A23L29/30A23L29/35A23L33/125A23L33/18A61P25/00A61P25/20A61P25/28A23J1/08A23L2/66A23L33/17
Inventor VAN BECKHOVEN, RUDOLF FRANCISCUS W.C.DUCHATEAU, ALEXANDER LUCIA L.EDENS, LUPPOKLEINHERENBRINK, FRANCISCUS ANTONIUS MKLOEK, JORISDE ROOS, ANDRE LEONARDUS
Owner CONOPCO INC D B A UNILEVER
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