Strains and methods for improving ruminant health and/or performance

Inactive Publication Date: 2010-07-08
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018]In at least some embodiments, the administration of the one or more strain to the animal provides at least one of the following benefits in or to the animal when compared to an animal not administered the strain: (a) reduces acidosis, (b) stabilizes ruminal metabolism as indicated by delayed lactic acid accumulation and prolonged production of volatile fatty acids, (c) recovers more quickly from acidosis challenge as measur

Problems solved by technology

Increasing the ruminant consumption of fermentable carbohydrate by feeding higher levels of cereal grains has resulted in increased incidence of metabolic disorders such as acidosis.
However, the effect of excessive carbohydrate on the ruminal microbiota that initiates this response has not been well documented.
Although intensive feeding management is usually quite effective in controlling acidosis, it is very costly to the producer due to the high cost of producing, transporting, chopping forage, disposing of increased animal waste, and lower production efficiencies.
However, these Enterococcus strains failed

Method used

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  • Strains and methods for improving ruminant health and/or performance
  • Strains and methods for improving ruminant health and/or performance
  • Strains and methods for improving ruminant health and/or performance

Examples

Experimental program
Comparison scheme
Effect test

example 1

Acidosis Model Experimental Design:

[0052]Ten crossbred steers were blocked by weight and assigned to two pens. The daily feed ration for all treatment groups prior to challenge consisted of 45% roughage and 55% concentrate on a dry matter basis. Cattle were fed 15 lbs / head / day of the ration once in the morning and had remaining feed pushed closer to the feeding stanchion late in the afternoon. Both pens were fasted for 24 hours before challenge with the concentrate diet treatments. Concentrate diet treatments consisted of highly fermentable carbohydrate sources of steam flaked corn on a 90% as fed basis. After fasting for 24 hours, the concentrate diet was fed ad libitum at 100 lbs / pen to all pens (0 h). Challenge diet consumption was visually monitored and additional feed added on an as needed basis.

[0053]Rumen fluid samples were obtained from individual animals via oral intubation using a collection tube attached to a vacuum flask. Different flasks and collection tubes were used f...

example 2

In Vitro Strain Testing:

[0067]Rumen fluid was collected for in vitro trials from two yearling Hereford heifers. Heifers were identified by identification tags and were referred to as 101 and 133. Heifers were fed 6 lbs / head / day of dried distillers grain (DDGS) and had access to free choice haylage.

[0068]The in vitro protocol was followed as closely as possible to decrease experimental error between each trial. Briefly, rumen fluid was collected from each heifer and placed into marked, pre-warmed thermoses. Thermoses were transported to Agtech Products, Inc. for processing. Rumen fluid was added in duplicate to bottles containing McDougall's Buffer and 3.0% glucose (final concentration after McDougall's Buffer and rumen fluid have been mixed to a volume of 180 ml), which had been tempered to 39° C. Candidate DFM strains, Enterococcus faecium strain 8G-1, Enterococcus faecium strain 8G-73, and Bacillus pumilus strain 8G-134, were added to designated bottles at 1.0×107 CFU / ml (final co...

example 3

Candidate DFM Testing in Cattle Fed a Readily Fermentable Carbohydrate—an Acute Acidosis Challenge:

[0072]Materials and Methods:

[0073]Cattle and Pens Assignments:

[0074]Twenty cross-bred beef steers were purchased at local sale barns. Cattle were housed at the research facility for a period of two weeks prior to trial initiation for observation of morbidity or mortality. Cattle were randomly blocked across treatment by weight. Five head of cattle were assigned to a pen and pens designated to one of four treatments. Treatments consisted of 3 pens each receiving a different DFM as is detailed below with the fourth pen receiving no DFM (control). Treatment assignments can be seen in Table 2 below.

TABLE 2Treatment assignments by pen.CandidateMinimum DosePen IDDFM (TX)(CFU / Head / Day)16s rRNA Identification1None0None (Control)28G-15 × 1010Enterococcus spp.38G-735 × 1010Enterococcus spp.48G-1345 × 109Bacillus pumilus

The daily feed ration for all treatment groups prior to challenge, consisted ...

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PUM

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Abstract

Described are strains including Enterococcus faecium strain 8G-1 (NRRL B-50173), Enterococcus faecium strain 8G-73 (NRRL B-50172), Bacillus pumilus strain 8G-134 (NRRL B-50174) and strains having all of the identifying characteristics of each of these strains. One or more of the strains can be used to treat or prevent acidosis. They can also be used to improve other measures of ruminant health and/or performance. Methods of using the strains, alone and in combination, are described. Methods of making the strains are also provided.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application No. 61 / 119,256, filed Dec. 2, 2008, the entirety of which is incorporated by reference herein.BIBLIOGRAPHY[0002]Complete bibliographic citations of the references referred to herein by the first author's last name and year of publication can be found in the Bibliography section, immediately preceding the claims.FIELD OF THE INVENTION[0003]The invention relates to strains and methods for controlling acidosis. More particularly, the invention relates to bacterial strains useful for improving ruminant health and / or performance and methods of making and using the strains.DESCRIPTION OF THE RELATED ART[0004]The feeding of high concentrations of fermentable carbohydrate to ruminants has become a common practice in the beef and dairy cattle industry over the last 50 years. The need for improving the production efficiency and quality of meat has led to...

Claims

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Application Information

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IPC IPC(8): A61K35/74C12N1/20A61K35/742A61K35/744
CPCA23K1/009A23K1/1813A61K35/742A61K35/744C12R1/07C12R1/46A61K2300/00A23K50/10A23K10/18C12R2001/07C12N1/205C12R2001/01A23K10/16A61P1/00A61P39/02C12R2001/46
Inventor MERTZ, KEITH J.REHBERGER, THOMAS G.
Owner DUPONT NUTRITION BIOSCIENCES APS
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