Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection method

a technology of hydrocarbon fuel and detection method, which is applied in the direction of material testing goods, biochemistry equipment and processes, biochemistry apparatus, etc., can solve the problems of affecting the analysis results, and affecting the quality of the analysis

Inactive Publication Date: 2013-05-23
CONIDIA BIOSCI
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a method and kit for analyzing liquid hydrocarbon fuel to detect the presence of a specific organism, called Hormoconis resinae. The method involves mixing the fuel sample with an aqueous diluent and antibodies specific to a metabolite produced by Hormoconis resinae. The resulting mixture is then incubated to allow the antibodies to bind to the metabolite. The presence or absence of bound antibodies is detected, indicating the presence of actively growing Hormoconis resinae. The kit contains a homogenized preparation of Hormoconis resinae grown in liquid hydrocarbon fuel, the specific antibodies, and an aqueous diluent with emulsifier. The technical effect is a reliable and fast method for detecting the presence of Hormoconis resinae in liquid hydrocarbon fuel samples.

Problems solved by technology

It is known that hydrocarbon fuel can become contaminated with micro-organisms, which is undesirable.
For example, Hormoconis resinae (the ‘Jet Fuel Fungus’) can grow on aviation kerosene causing corrosion and blockage problems in aircraft wing tanks.
All, however, are based on culture techniques which take 72 hours for a result to be obtained.
At present there is still no rapid test available for the presence of micro-organisms in hydrocarbon fuel.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

Analysis of H. resinae in Hydrocarbon Fuel Using ELISA

Materials

[0028]Antigen / Hormoconis resinae Preparation

[0029]The antigen preparation is used in both the coating of the microplates and production of the standards used in the ELISA test.

[0030]1 H. resinae is grown on malt extract agar (toffee barley malt extract 20 g, agar oxoid no. 3 20 g, 1 litre of tap water, pH 6.5, autoclaved at 121° C. for 15 minutes) for 7-10 days at 25° C.

[0031]2 A spore suspension is prepared in ‘Bushnell Haas’ (Difco) liquid broth.

[0032]Approximately 3 mls suspension per 100 mls fuel preparation are used as an inoculant as described below.

[0033]3 H. resinae is inoculated into in a fuel medium prepared by floating sterile susceptible fuel over sterile Bushnell Haas broth in the approximate ratio of 1:3.

[0034]4 The medium is incubated at 25° C. until a confluent fungal mat is obtained in the vessel.

[0035]5 The mat is filter harvested from the medium and washed in sterile distilled water.

[0036]6 The mat is ...

example 2

Analysis of Fuel Sample

[0084]Fuel samples were supplied by an airline for testing. 2 samples were known to have fungal contamination as tested by conventional culture assays. Samples were treated as outlined in Example 1. Samples were emulsified and run on the day of the assay. Results are shown in Table 1 below. The results of the ELISA assay confirm the culture assay, with samples 2082 / 32 and / 36 having high fungal content.

TABLE 1ELISA Assay forH. resinaeSamples IdentityConc. μg / mlConc. μg / 100 ml2082 / 32>200>200002082 / 3311002082 / 34>200302082 / 350.34342082 / 36134134002082 / 371.1802082 / 380.4402082 / 3900

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method of analysis of a hydrocarbon fuel for the presence of a micro-organism comprises contacting a fuel sample with an aqueous diluent and with an antibody reactive with the micro-organism, or reactive with a metabolite of breakdown product produced by the micro-organism, to detect the presence or absence of the micro-organism.

Description

RELATED APPLICATIONS[0001]This application is a continuation of U.S. patent application Ser. No. 10 / 468,691, filed Jan. 24, 2004, which is a national stage application filed under 35 USC§371 of PCT / GB02 / 00747, filed Feb. 21, 2002, which claims the benefit of Great Britain Patent Application No. GB 0104566.5, filed Feb. 23, 2001, all of which are incorporated herein, in entirety, by reference.FIELD OF THE INVENTION[0002]The invention relates generally to a method for analysis of hydrocarbon fuel.BACKGROUND OF THE INVENTION[0003]It is known that hydrocarbon fuel can become contaminated with micro-organisms, which is undesirable. For example, Hormoconis resinae (the ‘Jet Fuel Fungus’) can grow on aviation kerosene causing corrosion and blockage problems in aircraft wing tanks. Airlines are required to send samples to laboratories for testing if contamination is suspected. Most laboratories use standard culture techniques which can take up to ten days to confirm. There are so-called “ra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/22G01N33/569
CPCG01N33/22G01N33/56961
Inventor KELLY, JOAN
Owner CONIDIA BIOSCI