Compositions comprising textured patterns and methods of using the same

Inactive Publication Date: 2017-08-03
UNIV OF MARYLAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is about a method of guiding the movement of cells in a person's body. This is done by creating a surface with a pattern of asymmetric topographical elements that can be seen or felt by the cells. These elements cause the cells to move in a specific direction when they align with the pattern. The pattern can be created using protrusions that are saw-tooth shaped, columnar, ridge or pyramidal shaped. The pattern can be created using a three-dimensional pattern of straight, curved or circumferential paths. The cells can be moved across a distance of at least about 5 microns. The invention also includes a composition that can be used to induce healing of wounds in a person's body by creating a surface with the asymmetric pattern.

Problems solved by technology

Gradient-based cell guidance approaches share a crucial limitation: To maintain an uphill gradient in a physical or chemical property of the environment, the absolute value of the physical property or chemical concentration must continuously change with position.
Combined with a limited dynamic sensing range, this limits the practical range of e.g. chemotaxis to mm length-scales.
However, inducing unidirectional cell migration using contact guidance remains challenging.
As the actin waves couple to a substrate composed of three-dimensional asymmetric nano-topography with a temporal periodicity, it is possible that the asymmetric topography reorganizes and biases the actin waves, leading to unidirectional cell migration.

Method used

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  • Compositions comprising textured patterns and methods of using the same
  • Compositions comprising textured patterns and methods of using the same
  • Compositions comprising textured patterns and methods of using the same

Examples

Experimental program
Comparison scheme
Effect test

example 2

of MCF10A Healthy Breast Tissue Cells

[0111]FIG. 12A-12H shows analysis of cell tracks on a flat surface and on 1.5 μm spaced nanoridges by measuring contact guidance of MCF10A cells. MCF10A cells are derived from breast tissue of a healthy patient. Phase-contrast images of the cells with a scale bar of 100 μm are shown in FIGS. 12A and 12B. Individual cell tracks (show in different grayscale colors for each cell) from a representative movie are shown over 10 h with scale bar 100 μm in FIG. 12C and FIG. 12D. The normalized velocity direction distribution is shown for all cells in all frames over all movies with a black curve for the flat surface and a gray curve for the nanoridge surface (FIG. 12E). The mean first-passage time for cells to reach a displacement r is shown in (FIG. 12F) with the same colors as (FIG. 12E); error bars indicate standard error of the mean across cells. In FIGS. 12E and 12F, there are n=150 cells on the flat surface and n=141 cells on the ridged surface fro...

example 3

. Preparation of Medical Adhesive Bandages Containing an Absorbent Pad with a Sawtooth Surface

[0112]Medical adhesive bandages comprising a backing, an absorbent pad, and a pressure sensitive adhesive to maintain the medical adhesive bandage in place will be prepared. Preparation of medical adhesive bandages is described, for example, in US2007 / 001077, which is incorporated by reference herein in its entirety. The backing will contain two major surfaces, i.e., an outer major surface and an inner major surface, a reinforcing scrim, and a layer of pressure-sensitive adhesive disposed on the inner major surface thereof. Backing materials that will be evaluated include polyethylene, other polyolefins, and polyurethane polymers and blends of such materials. Absorbent pads manufactured from a number of materials will be evaluated, including woven and nonwoven cotton, rayon, nonwovens, hydrocolloids, foams, and combinations thereof. Sawtooth surfaces will be applied to the absorbent pad usi...

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Abstract

The invention provides method of guiding unidirectional movement of a cell comprising exposing the cell to a surface comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements for a time period sufficient to bias actin polymerization within the cell. The invention also provides methods of inducing directional movement of a cell, and methods of harnessing an actin wave within a cell. In addition, the invention provides compositions comprising a contact side, wherein at least a portion of the contact side comprises a cell contact portion comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements, and methods of inducing healing of a wound of a subject comprising contacting the wound with the composition.

Description

RELATED APPLICATIONS[0001]This application claims priority to U.S. Provisional Patent Application Ser. No. 62 / 288,134 filed on Jan. 28, 2016, the contents of which are incorporated herein by reference in their entirety.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH[0002]This invention was made with government support under R01GM085574 awarded by the National Institutes of Health (NIH). The government has certain rights in the invention.FIELD OF THE DISCLOSURE[0003]The disclosure relates generally to compositions comprising textured patterns and methods of using the same for induction of unidirectional movement of one or a plurality of cells The disclosure also relates to method of making and using the textured nanoscale surface for wound healing when configured for application or presentation on a wound dressing, such as a bandage.BACKGROUND[0004]Cell migration is essential in many critical physiological processes (Ridley et al., 2003, Science 302: 1704) such as embryonic developm...

Claims

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Application Information

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IPC IPC(8): A61L15/42A61L15/58A61L27/50
CPCA61L15/42A61L2400/18A61L15/58A61L27/50A61F13/0243
Inventor LOSERT, WOLFGANGFOURKAS, JOHN T.DRISCOLL, MEGHANBATES, II, FLOYDSUN, XIAOYUGUVEN, CANPARKER, JOSHUA
Owner UNIV OF MARYLAND
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