Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhizomania-resistant gene

a technology of rhizomania and resistance, applied in the field of rhizomania resistance gene, can solve the problems of insufficient resistance conveyed by rz-1 in heavily infected regions or regions, loss of earnings of 50% and more, and inability to adapt to the environment, etc., to achieve the effect of reducing the cost of cropping

Active Publication Date: 2018-09-27
KWS SAAT SE & CO KGAA
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a nucleic acid molecule and / or a polypeptide that can convey resistance against rhizomania in a plant. This allows for the development of new resistant plant lines and the cultivation of new resistant species of the Beta genus. The use of molecular markers enables efficient cultivation against rhizomania and the development of new resistance markers. The invention also provides methods for selecting a plant with resistance to BNYVV using the resistant gene allele. The use of the identified resistant RZ3 gene allele in a genetic or molecular stack with other genetic elements can lead to the development of new resistance alleles and the creation of new cultivation areas for crop plants. The invention also provides genetic elements that can be used to convey various other agricultural properties, such as tolerance to herbicides, pathogens, cool temperatures, frost, water utilisation efficiency, nitrogen utilisation efficiency, and harvest.

Problems solved by technology

Rhizomania is the most serious sugar beet disease worldwide in terms of profitability and may cause earnings losses of 50% and more.
When the infection spreads to the entire plant, this results in a yellowing of the leaf veins, in necrotic lesions, and yellow flecks on the leaves.
It has been found, however, that the resistance conveyed by RZ-1 is insufficient in heavily infected regions or in regions having diverse BNYVV pathotypes (for example Sohi & Maleki, 2004).
In addition, the sequence region, in particular around this resistance gene, is repetitive, which makes the development of diagnostic markers particularly difficult.
In addition, the functional background of these resistance genes, i.e. the genetic structure, previously was not fully known.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhizomania-resistant gene
  • Rhizomania-resistant gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

Mapping and Fine-Mapping of the RZ-3 Gene / Genetic Physical Map

[0063]The RZ-3 resistance (also referred to as C48 resistance or C48) was mapped in a number of steps by means of mapping and fine-mapping on chromosome 3 between 57.1 and 57.8 cM (internal reference map), i.e. at a genetic distance between two flanking markers of 0.0714 cM in the genetic map. A total of 8004 plants for the crossing S504 (sensitive genotype)×T74 (resistant genotype) were examined for the mapping. Parallel to the C48 QTL mapping new informative markers were developed in a target-oriented manner following each mapping step and were used for the limitation of the C48 target region. The fine-mapping coordinates were additionally confirmed with the analysis of the descendants of the informative recombinants. Informative recombinant BC2S1 or BC2S2 plants were analysed intensively for this purpose, in each case with 90-180 descendants. These descendants were genotyped and phenotyped in parallel on an individual ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the Beta genus, in which the polypeptide is expressed, and also a preferred nucleic acid molecule encoding the RZ-3 gene of Beta maritima, derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, methods for production thereof, and methods for identifying a resistance-conveying nucleic acid molecule.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a divisional application of U.S. patent application Ser. No. 14 / 899,416, filed Dec. 17, 2015, which is a U.S. National Phase of International Patent Application Serial No. PCT / DE2014 / 000310, filed on Jun. 6, 2014, which claims the benefit of German Patent Application Serial No. 102013010026.7, filed on Jun. 17, 2013, all of which applications are hereby incorporated by reference in their entireties.SEQUENCE LISTING[0002]The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Aug. 12, 2016, is named 245761_000015_SL.txt and is 327,226 bytes in size.FIELD OF THE INVENTION[0003]The present invention relates to a nucleic acid molecule that encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82C07K14/415C12Q1/6895
CPCC12Q2600/156C12Q2600/13C07K14/415C12N15/8283C12Q1/6895
Inventor TORJEK, OTTOBORCHARDT, DIETRICHMECHELKE, WOLFGANGLEIN, JENS CHRISTOPH
Owner KWS SAAT SE & CO KGAA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com