Rhizomania-resistant gene

Abstract

The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the Beta genus, in which the polypeptide is expressed, and also a preferred nucleic acid molecule encoding the RZ-3 gene of Beta maritima, derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, methods for production thereof, and methods for identifying a resistance-conveying nucleic acid molecule.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a divisional application of U.S. patent application Ser. No. 14 / 899,416, filed Dec. 17, 2015, which is a U.S. National Phase of International Patent Application Serial No. PCT / DE2014 / 000310, filed on Jun. 6, 2014, which claims the benefit of German Patent Application Serial No. 102013010026.7, filed on Jun. 17, 2013, all of which applications are hereby incorporated by reference in their entireties.SEQUENCE LISTING[0002]The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Aug. 12, 2016, is named 245761_000015_SL.txt and is 327,226 bytes in size.FIELD OF THE INVENTION[0003]The present invention relates to a nucleic acid molecule that encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular o...

AI Technical Summary

Benefits of technology

The present invention provides a nucleic acid molecule and / or a polypeptide that can convey resistance against rhizomania in a plant. This allows for the development of new resistant plant lines and the cultivation of new resistant species of the Beta genus. The use of molecular markers enables efficient cultivation against rhizomania and the development of new resistance markers. The invention also provides methods for selecting a plant with resistance to BNYVV using the resistant gene allele. The use of the identified resistant RZ3 gene allele in a genetic or molecular stack with other genetic elements can lead to the development of new resistance alleles and the creation of new cultivation areas for crop plants. The invention also provides genetic elements that can be used to convey various other agricultural properties, such as tolerance to herbicides, pathogens, cool temperatures, frost, water utilisation efficiency, nitrogen utilisation efficiency, and harvest.

Problems solved by technology

Rhizomania is the most serious sugar beet disease worldwide in terms of profitability and may cause earnings losses of 50% and more.

When the infection spreads to the entire plant, this results in a yellowing of the leaf veins, in necrotic lesions, and yellow flecks on the leaves.

It has been found, however, that the resistance conveyed by RZ-1 is insufficient in heavily infected regions or in regions having diverse BNYVV pathotypes (for example Sohi & Maleki, 2004).

In addition, the sequence region, in particular around this resistance gene, is repetitive, which makes the development of diagnostic markers particularly difficult.

In addition, the functional background of these resistance genes, i.e. the genetic structure, previously was not fully known.

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