Method for rapidly and selectively culturing cold-resistant high-quality king rice variety in rice growing region of Heilongjiang

Abstract

The invention relates to a method for rapidly and selectively culturing a cold-resistant high-quality king rice variety in a rice growing region of Heilongjiang. The method comprises the following technological steps of: by utilizing a high-quality gene variety containing medium amylose content, flexible gel consistency and fragrance as a donor parent, and a main cultivar variety of Heilongjiang as a receptor recurrent parent, performing one-generation hybridization and one-generation backcrossing so as to obtain BC1F1; performing SSR analysis on genetic background of BC1F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds to backcross with the recurrent parent so as to obtain BC2F1; obtaining BC3F1 by use of a same method; performing SSR analysis on genetic background of BC3F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds, and bagging for selfing, thereby obtaining BC3F2; performing SSR analysis on genetic background of BC3F2 single plants, screening out a plant which contains homozygous target genes and more receptor genetic backgrounds, meanwhile performing cold-resistant screening and identification by use of a 72-hour cold water pooling method before about 15 days of head sprouting, and performing rice quality detection and identification in a laboratory.

Description

technical field [0001] The invention proposes a method for cultivating new varieties of rice with molecular marker-assisted selection, rapid enhancement of cold resistance and improvement of eating quality. It only takes 5 generations to control the rice medium amylose content, soft gel consistency, and aroma genes to be directed into the recipient variety, while retaining other characteristics of the recipient variety. The procedures and steps of cultivating high-quality new rice varieties are adopted by combining hybridization, backcrossing, molecular marker detection and rice quality analysis. While ensuring the introduction of the target gene of the donor, more of the genetic background of the recipient is retained, and the improved high-quality New varieties can be bred efficiently. Background technique [0002] The main goals of rice improvement in Heilongjiang rice region are cold resistance, high yield, high quality, and multi-resistance. However, with the continuo...

AI Technical Summary

Problems solved by technology

[0004] The conventional method of rice breeding for eating quality is inefficient, and rice quality is a comprehensive expression of multiple traits, and each trait is difficult to control, and is greatly affected by the environment. Accelerating the selection process of high-quality rice and improving the breeding efficiency of high-quality rice are important problems that need to be solved urgently in rice quality breeding