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Molecular breeding method for breeding new anti-verticillium cotton seed

A technology for molecular breeding and resistance to Verticillium wilt, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection, etc. , to achieve the effect of long breeding cycle and poor selection reliability

Active Publication Date: 2012-06-27
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Breeders have always wanted to introduce Verticillium wilt resistance genes from sea-island cotton into upland cotton through crossbreeding between sea and land species, but the breeding of disease-resistant varieties has been limited due to linkage cumbersomeness and the difficulty of stabilizing hybrid offspring.

Method used

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  • Molecular breeding method for breeding new anti-verticillium cotton seed
  • Molecular breeding method for breeding new anti-verticillium cotton seed
  • Molecular breeding method for breeding new anti-verticillium cotton seed

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Embodiment 1

[0022] Embodiment 1 selects parents

[0023]The Cotton Research Institute of the State Key Laboratory of Crop Genetics and Germplasm Innovation of Nanjing Agricultural University uses the genetic standard line TM-1 of upland cotton as the recurrent parent, and the disease-resistant and high-quality Sea Island Mianhai 7124 line as the donor parent (non-recurrent parent). Combined with the SSR molecular marker-assisted selection covering the whole genome, the first domestic upland cotton genetic standard line TM-1 background 174 sea-island cotton chromosome segment introduction lines were bred, and the genome coverage rate reached 83.5%. Verticillium wilt strains V991 and V07DF2 are two deciduous strains with different pathogenicity in the Yangtze River cotton region (the strains are preserved by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences), and Verticillium wilt strain D8092 is a medium pathogenic strain in the Yellow River cotton region. Deciduo...

Embodiment 2

[0025] (1) In the summer of 2008, a row of IL095 and IL154 were planted at the Jiangpu Experimental Station of Nanjing Agricultural University. IL095 (CGMCC NO.5574) was used as the female parent and IL154 (CGMCC NO.5575) was used as the male parent to prepare a hybrid combination to produce F 1 , all mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2008 1 , selfing to obtain F 2 , all mixed harvest after maturity. Field management was carried out by conventional methods.

[0026] (2) Molecular marker-assisted selection: In the summer of 2009, 50 rows of F 2 , 10-12 plants per line, 3-4 unexpanded leaves were collected from each plant and put into a 1.5ml eppendorf tube, and 600 μl of pre-cooled freshly prepared extraction buffer was added, ground with an electric drill, and CTAB method (Paterson AH , Brubaker CL, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis [J]. Plant ...

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Abstract

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for breeding a new anti-verticillium cotton seed. The method comprises the following steps: taking a chromosome segment introgression line 1L095 of sea-island cotton of which the collection number is CGMCC No.5574 as a female parent and a chromosome segment introgression line 1L154 of sea-island cotton of which the collection number is CGMCC No.5575 as a male parent to crossbreed a generation I seed, and obtaining F2 from the crossbred generation I seed, wherein each generation is collected bya single strain; and selecting by taking a molecular marker for assistance to obtain a novel multi-strain prevention cotton strain. Through the adoption of the method, the novel multi-strain prevention cotton strain-Nannong haidao V is bred. As the molecular marker is taken for assistance to select a target trait, the novel multi-strain prevention cotton strain can be bred in 2 to 3 years fast and effectively. The corresponding disease index of Nannong haidao V is 15.51 to 20.97 percent.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for cultivating a new variety of cotton resistant to Verticillium wilt, in particular to a molecular breeding method for cultivating a new cotton variety resistant to Verticillium wilt by using a sea-island cotton chromosome segment introduction line. Background technique [0002] Cotton is an important economic crop worldwide. China is a big cotton producer, consumer and textile country. As a major cotton-producing country, my country's main cotton-producing areas cover 16 provinces, municipalities (autonomous regions), with an annual cotton planting area of ​​70-80 million mu, with a total output of about 6 million tons, accounting for a quarter of the world's total output. However, cotton is threatened by various diseases during the growing period, such as Verticillium wilt, known as cotton "cancer", which has a global distribution and causes seriou...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02A01H1/04
Inventor 张天真王鹏郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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