Molecular breeding method for breeding new anti-verticillium cotton seed

A technology for molecular breeding and resistance to Verticillium wilt, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection, etc. , to achieve the effect of long breeding cycle and poor selection reliability

Active Publication Date: 2012-06-27
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Breeders have always wanted to introduce Verticillium wilt resistance genes from sea-island cotton into upland cotton through crossbreeding between sea and la

Method used

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  • Molecular breeding method for breeding new anti-verticillium cotton seed
  • Molecular breeding method for breeding new anti-verticillium cotton seed
  • Molecular breeding method for breeding new anti-verticillium cotton seed

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0022] Example 1 Selection of Parents

[0023] The National Key Laboratory of Crop Genetics and Germplasm Innovation, Nanjing Agricultural University, Cotton Research, therefore, upland cotton genetic standard line TM-1 is the recurrent parent, and the disease-resistant, high-quality sea island cotton Hai 7124 is the donor parent (non-recurrent parent). The Jiao Gao generation combined with SSR molecular marker-assisted selection covering the whole genome, and cultivated the first domestic set of upland cotton genetic standard line TM-1 background to introduce 174 sea island cotton chromosome fragment introduction lines, with a genome coverage of 83.5%. Verticillium wilt strains V991 and V07DF2 are two deciduous strains with different pathogenicity in the Yangtze River cotton area (strains are preserved by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences), and the Verticillium wilt strain D8092 is a moderately pathogenic strain in the Yellow River cotton...

Example Embodiment

[0024] Example 2

[0025] (1) In the summer of 2008, a row of IL095 and IL154 were planted at Jiangpu Experimental Station of Nanjing Agricultural University. IL095 (CGMCC NO.5574) was the female parent, and IL154 (CGMCC NO.5575) was the male parent to produce F. 1 , All mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2008 1 , Get F from self-crossing 2 , All mixed harvest after maturity. Field management is carried out according to conventional methods.

[0026] (2) Molecular marker-assisted selection: 50 rows of F were planted at Jiangpu Experimental Station of Nanjing Agricultural University in the summer of 2009 2 , 10-12 plants per row, 3-4 unexpanded leaves from each plant are collected and placed in a 1.5ml eppendorf tube, and 600μl of pre-cooled freshly prepared extraction buffer is added, ground with an electric drill, and the CTAB method (Paterson AH , Brubaker CL, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) ge...

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Abstract

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for breeding a new anti-verticillium cotton seed. The method comprises the following steps: taking a chromosome segment introgression line 1L095 of sea-island cotton of which the collection number is CGMCC No.5574 as a female parent and a chromosome segment introgression line 1L154 of sea-island cotton of which the collection number is CGMCC No.5575 as a male parent to crossbreed a generation I seed, and obtaining F2 from the crossbred generation I seed, wherein each generation is collected bya single strain; and selecting by taking a molecular marker for assistance to obtain a novel multi-strain prevention cotton strain. Through the adoption of the method, the novel multi-strain prevention cotton strain-Nannong haidao V is bred. As the molecular marker is taken for assistance to select a target trait, the novel multi-strain prevention cotton strain can be bred in 2 to 3 years fast and effectively. The corresponding disease index of Nannong haidao V is 15.51 to 20.97 percent.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for cultivating a new variety of cotton resistant to Verticillium wilt, in particular to a molecular breeding method for cultivating a new cotton variety resistant to Verticillium wilt by using a sea-island cotton chromosome segment introduction line. Background technique [0002] Cotton is an important economic crop worldwide. China is a big cotton producer, consumer and textile country. As a major cotton-producing country, my country's main cotton-producing areas cover 16 provinces, municipalities (autonomous regions), with an annual cotton planting area of ​​70-80 million mu, with a total output of about 6 million tons, accounting for a quarter of the world's total output. However, cotton is threatened by various diseases during the growing period, such as Verticillium wilt, known as cotton "cancer", which has a global distribution and causes seriou...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02A01H1/04
Inventor 张天真王鹏郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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