Molecular breeding method for breeding new anti-verticillium cotton seed
A technology for molecular breeding and resistance to Verticillium wilt, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection, etc. , to achieve the effect of long breeding cycle and poor selection reliability
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[0022] Example 1 Selection of Parents
[0023] The National Key Laboratory of Crop Genetics and Germplasm Innovation, Nanjing Agricultural University, Cotton Research, therefore, upland cotton genetic standard line TM-1 is the recurrent parent, and the disease-resistant, high-quality sea island cotton Hai 7124 is the donor parent (non-recurrent parent). The Jiao Gao generation combined with SSR molecular marker-assisted selection covering the whole genome, and cultivated the first domestic set of upland cotton genetic standard line TM-1 background to introduce 174 sea island cotton chromosome fragment introduction lines, with a genome coverage of 83.5%. Verticillium wilt strains V991 and V07DF2 are two deciduous strains with different pathogenicity in the Yangtze River cotton area (strains are preserved by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences), and the Verticillium wilt strain D8092 is a moderately pathogenic strain in the Yellow River cotton...
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[0024] Example 2
[0025] (1) In the summer of 2008, a row of IL095 and IL154 were planted at Jiangpu Experimental Station of Nanjing Agricultural University. IL095 (CGMCC NO.5574) was the female parent, and IL154 (CGMCC NO.5575) was the male parent to produce F. 1 , All mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2008 1 , Get F from self-crossing 2 , All mixed harvest after maturity. Field management is carried out according to conventional methods.
[0026] (2) Molecular marker-assisted selection: 50 rows of F were planted at Jiangpu Experimental Station of Nanjing Agricultural University in the summer of 2009 2 , 10-12 plants per row, 3-4 unexpanded leaves from each plant are collected and placed in a 1.5ml eppendorf tube, and 600μl of pre-cooled freshly prepared extraction buffer is added, ground with an electric drill, and the CTAB method (Paterson AH , Brubaker CL, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) ge...
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