Composition for preventing, amelioreating, or treating stress or depression comprising medicinal herb extract as effective ingredient

Pending Publication Date: 2021-07-15
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010]The present invention relates to a composition for preventing, ameliorating, or treating stress or depression caused by stress comprising medicinal herb extract as an effective ingredient. Specifically, it is shown that an extrac

Problems solved by technology

In addition, due to the pressure relating to hard study or the like,

Method used

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  • Composition for preventing, amelioreating, or treating stress or depression comprising medicinal herb extract as effective ingredient
  • Composition for preventing, amelioreating, or treating stress or depression comprising medicinal herb extract as effective ingredient
  • Composition for preventing, amelioreating, or treating stress or depression comprising medicinal herb extract as effective ingredient

Examples

Experimental program
Comparison scheme
Effect test

Example

EXAMPLE 1

Preparation of extract of Hibiscus syriacus flower or Hibiscus syriacus Root

[0034]The Hibiscus syriacus flower used in the present invention was collected from the area of Daejon in Korea, and roots of rose of Sharon were purchased from a tree farm in Youngchon of Kyungbuk in Korea and used as Hibiscus syriacus root. The Hibiscus syriacus flower (250 g) was added with 30% (v / v) ethanol (20× volume) and extracted under reflux at 90±2° C. for 3 hours. Furthermore, 1.8 kg of the Hibiscus syriacus root were added with 70% (v / v) ethanol (10× volume) and extracted under reflux at 84±2° C. for 3 hours. After the first filtering using a filtering wire, the second filtering was carried out by using cotton to have a concentrate, which was then subjected to freeze-drying followed by pulverization. After the concentration, the Hibiscus syriacus flower was obtained at a yield of 27.6%, and the Hibiscus syriacus root was obtained at a yield of 7.2%.

Example

EXAMPLE 2

Cell culture

[0035]SK-N-SH cells as human neuroblastoma cell line were obtained from Korean Cell Line Bank, cultured in DMEM (Dulbecco's modified Eagle's medium) medium containing 10% bovine serum and 1% penicillin / streptomycin (37° C., 5% CO2 incubator), and used. Once a cell monolayer is formed, the cells were treated with TrypLE 1× solution to release the cells followed by subculture.

Example

EXAMPLE 3

Determination of Cytotoxicity and Measurement of Activity of Protecting Nerve Cells

[0036]Cytotoxicity of an extract of Hibiscus syriacus flower and Hibiscus syriacus root, which is an effective ingredient of the present invention, was determined in human neuroblastoma cell. On a Corning BioCoat collagen I 96-well plate, SK-N-SH cells were sewn at 3×104 / well. Thereafter, the cells were treated for 24 hours with an extract of Hibiscus syriacus flower or Hibiscus syriacus root at a concentration of 10, 50, 100, or 200 μg / ml. Then, MTS assay was carried out and, by measuring the absorbance using ELISA (enzyme-linked immunosorbent assay) plate reader, the cell viability was determined.

[0037]As a result, it was confirmed that the extract of Hibiscus syriacus flower or Hibiscus syriacus root exhibits absolutely no cytotoxicity as it is shown in FIG. 1.

[0038]Furthermore, based on the toxicity of corticosterone, the activity of protecting nerve cells in human blastoma was examined. ...

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Abstract

A method for preventing, ameliorating, or treating stress or depression includes administering a subject in need thereof a composition including a medicinal herb extract as an effective ingredient. Tt is shown that an extract of Hibiscus syriacus flower and an extract of Hibiscus syriacus root have an activity of protecting nerve cells, can lower the expression level of an inflammatory factor, which is increased by stress, and is effective for depression caused by stress.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for preventing, ameliorating, or treating stress or depression comprising medicinal herb extract as an effective ingredient.BACKGROUND ART[0002]In accordance with a rapid change and diversification of the society, modern-day people are asked to cover multiple roles. Accordingly, there are more and more people suffering from mental illness like depression, anxiety disorder, or sleep disorder that are caused by various stresses. According to the year 2006 epidemiologic study carried out by Ministry or Health and Welfare of South Korea regarding mental illness in which adults with age of from 18 to 64 are examined as a subject, “one-year prevalence of mental illness” is found to be 17.1% in South Korea. Furthermore, “lifetime prevalence of mental illness”, which indicates the proportion of a population who, at some point during their lifetimes, has ever experienced at least one mental illness is 30%, i.e., one in three...

Claims

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Application Information

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IPC IPC(8): A61K36/185A23L33/105A61P25/24A61K9/00
CPCA61K36/185A23L33/105A61K2236/51A61K9/0053A61K2236/33A61P25/24A61K9/0019A61K47/10A61K47/36A23V2002/00A23V2200/322
Inventor LEE, MI YOUNGCHOI, GOYAIM, A RANGKIM, YOUNG HWACHAE, SUNG WOOK
Owner KOREA INST OF ORIENTAL MEDICINE
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