Detector for liquid chromatography

Abstract

A detector for liquid chromatography has light sources that generate light in an ultraviolet region and in a near-infrared region; a flow cell, through which sample liquid flows; an optical system to let light generated from the light sources become incident on the flow cell concurrently; a detection element that detects light in the ultraviolet region that passes through the flow cell; a detection element that detects light in the near-infrared region that passes through the flow cell; and an arithmetic operation part that performs arithmetic operation of a first signal value obtained from the detection element and of a second signal value obtained from the detection element. The arithmetic operation part combines the first signal value and the second signal value to calculate a signal value with a reduced baseline fluctuation resulting from a mobile phase during a gradient analysis.

Description

CLAIM OF PRIORITY[0001]The present application claims priority from Japanese patent application JP 2013-204590 filed on Sep. 30, 2013, the content of which is hereby incorporated by reference into this application.BACKGROUND[0002]Technical Field[0003]The present invention relates to a detector for liquid chromatography and a method to correct a detection signal thereof.[0004]Background Art[0005]In general-purpose liquid analyzers including a liquid chromatograph, absorbance detectors have been widely used because of its high detection sensitivity and user-friendliness. Especially an ultraviolet wavelength band that is short wavelengths of 300 nm or less includes excitation wavelengths of various functional groups in organic compounds, and so all industrially-useful organic compounds can be a detection target in such a wavelength band. Specifically transitions of σ→σ*, n→σ*, n→π* and π→σ* correspond to electron transitions in this wavelength region, in which hydroxyl groups, carboxyl...

AI Technical Summary

Benefits of technology

[0016]Problems, configurations, and advantageous effects other than those described above will be made clear by the following description of embodiments.

other than those described above will be made clear by the following

Problems solved by technology

Such an ultraviolet-region absorbance detector, however, has a problem that its baseline changes greatly when a gradient analysis is applied in the liquid chromatographic field, thus failing to yield a good chromatogram.

Since such an organic solvent has a strong absorption in the ultraviolet wavelength band compared with water, the baseline changes with the change in composition ratio of the organic solvent during a gradient analysis, and so the applicable range thereof is limited.

Images