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Anti-C5 antibodies

a technology of anti-c5 antibodies and antibodies, applied in the field of anti-c5 antibodies, can solve the problems of marked impairment of the plasma retention of igg, and achieve the effect of enhancing the clearance of c5

Active Publication Date: 2017-09-19
CHUGAI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0036]Anti-C5 antibodies of the present invention may be used in the manufacture of a medicament. In some embodiments, the medicament is for treatment of a complement-mediated disease or condition which involves excessive or uncontrolled activation of C5. In some embodiments, the medicament is for enhancing the clearance of C5 from plasma.

Problems solved by technology

When the FcRn binding ability of an IgG under acidic conditions is eliminated by introducing mutations into its Fc region, the IgG is not recycled from the endosomes into the plasma, leading to marked impairment of the plasma retention of the IgG.

Method used

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Examples

Experimental program
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Effect test

example 1

Preparation of C5

1.1. Expression and Purification of Recombinant Human and Cynomolgus Monkey C5

[0354]Recombinant human C5 (NCBI GenBank accession number. NP_001726.2, SEQ ID NO: 39) was expressed transiently using FreeStyle293-F cell line (Thermo Fisher, Carlsbad, Calif., USA). Conditioned media expressing human C5 was diluted with equal volume of milliQ water, then applied to a Q-sepharose FF or Q-sepharose HP anion exchange column (GE healthcare, Uppsala, Sweden), followed by elution with a NaCl gradient. Fractions containing human C5 were pooled, then salt concentration and pH was adjusted to 80 mM NaCl and pH6.4, respectively. The resulting sample was applied to a SP-sepharose HP cation exchange column (GE healthcare, Uppsala, Sweden) and eluted with a NaCl gradient. Fractions containing human C5 were pooled and subjected to CHT ceramic Hydroxyapatite column (Bio-Rad Laboratories, Hercules, Calif., USA). Human C5 eluate was then applied to a Superdex 200 gel filtration column (G...

example 2

Generation of Anti-C5 Antibodies

2.1. Antibody Screening

[0357]Anti-C5 antibodies were prepared, selected and assayed as follows:

[0358]Twelve to sixteen week old NZW rabbits were immunized intradermally with human C5 and / or monkey C5 (50-100 μg / dose / rabbit). This dose was repeated 4-5 times over a 2 month period. One week after the final immunization, the spleen and blood were collected from the immunized rabbits. Antigen-specific B-cells were stained with labelled antigen, sorted with FCM cell sorter (FACS aria III, BD), and plated in 96-well plates at one cell / well density together with 25,000 cells / well of EL4 cells (European Collection of Cell Cultures) and activated rabbit T-cell conditioned medium diluted 20 times, and were cultured for 7-12 days. EL4 cells were treated with mitomycin C (Sigma, Cat No. M4287) for 2 hours and washed 3 times in advance. The activated rabbit T-cell conditioned medium was prepared by culturing rabbit thymocytes in RPMI-1640 containing Phytohemagglut...

example 3

Binding Characterization of Anti-C5 Antibodies

3.1. Expression and Purification of Recombinant Antibodies

[0369]Recombinant antibodies were expressed transiently using FreeStyle293-F cell line (Thermo Fisher, Carlsbad, Calif., USA). Purification from the conditioned media expressing antibodies was performed using a conventional method using protein A. Gel filtration was further conducted if needed.

3.2. Assessment of pH Dependency

[0370]The kinetic parameters of anti-C5 antibodies against recombinant human C5 were assessed at pH7.4 and pH5.8, at 37° C. using BIACORE® T200 instrument (GE Healthcare). ProA / G (Pierce) was immobilized onto a CM4 sensorchip using amine coupling kit (GE Healthcare) according to the recommended settings by GE Healthcare. Antibodies and analytes were diluted into the respective running buffers, ACES pH7.4 and pH5.8 (20 mM ACES, 150 mM NaCl, 1.2 mM CaCl2, 0.05% Tween 20, 0.005% NaN3). Each antibody was captured onto the sensor surface by ProA / G. Antibody capture...

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Abstract

An objective of the invention is to provide anti-C5 antibodies and methods of using the same. The invention provides anti-C5 antibodies and methods of using the same. In some embodiments, an isolated anti-C5 antibody of the present invention binds to an epitope within the β chain of C5 with a higher affinity at neutral pH than at acidic pH. The invention also provides isolated nucleic acids encoding an anti-C5 antibody of the present invention. The invention also provides host cells comprising a nucleic acid of the present invention. The invention also provides a method of producing an antibody comprising culturing a host cell of the present invention so that the antibody is produced. The invention further provides a method of producing an anti-C5 antibody comprising immunizing an animal against a polypeptide which comprises the MG1-MG2 domain of the β chain of C5. Anti-C5 antibodies of the present invention may be for use as a medicament. Anti-C5 antibodies of the present invention may be for use in treating a complement-mediated disease or condition which involves excessive or uncontrolled activation of C5. Anti-C5 antibodies of the present invention may be for use in enhancing the clearance of C5 from plasma.

Description

REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY[0001]The content of the electronically submitted sequence listing in ASCII text file NAME_SeqList.txt (3467_0130000 SL_ST25.txt; Size: 146 KB; and Date of Creation: Dec. 16, 2015) filed with the application is herein incorporated by reference in its entirety.BACKGROUND[0002]The present invention relates to anti-C5 antibodies and methods of using the same. The complement system plays a central role in the clearance of immune complexes and in immune responses to infectious agents, foreign antigens, virus-infected cells and tumor cells. There are about 25-30 complement proteins, which are found as a complex collection of plasma proteins and membrane cofactors. Complement components achieve their immune defensive functions by interacting in a series of intricate enzymatic cleavages and membrane binding events. The resulting complement cascades lead to the production of products with opsonic, immunoregulatory, and lytic functions.[00...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K39/395G01N33/68G01N33/564C07K16/18A61K31/519
CPCC07K16/18A61K31/519G01N33/564G01N33/6863C07K2317/21C07K2317/24C07K2317/76C07K2317/92C07K2317/94G01N2333/4716A61P13/02A61P13/12A61P15/06A61P17/00A61P19/02A61P25/00A61P27/02A61P29/00A61P37/02A61P43/00A61P7/00A61P9/00A61P9/10A61K2039/505C12N15/02
Inventor RUIKE, YOSHINAOSAMPEI, ZENJIRO
Owner CHUGAI PHARMA CO LTD
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