The invention provides a manufacturing method of iodoform gauze. The manufacturing method comprises the following steps of (1) preparing sterile gauze and a sterile bag comprising a brown 
bottle, a larger container, a large treatment disk, a surgical gown, a 
mortar and a 
grinding rod thereinto; and preparing absolute ethyl 
alcohol, iodoform, medical glycerin and 
diethyl ether, wherein the volume ratio of the absolute ethyl 
alcohol, the medical glycerin to the 
diethyl ether 1:1:1; (2) opening the sterile bag, pouring the gauze into the large treatment disk, adding the absolute ethyl 
alcohol to the disk and uniformly 
rubbing the gauze; and then pouring the medical glycerin and the iodoform into the 
mortar, uniformly stirring by using the 
grinding rod, pouring mixture onto the gauze in the large treatment disk, and uniformly 
rubbing till the gauze totally becomes yellow; and (3) spreading little 
diethyl ether on the gauze, rapidly stirring, and putting the ready-made gauze in the prepared brown 
bottle by a pair of 
tweezers for sealing 
cold storage. The manufacturing method of the iodoform gauze has the technical effects that the iodoform is not dissolved in the diethyl 
ether in advance, so that the content of the diethyl 
ether in the iodoform gauze is reduced, and the iodoform gauze nearly contains no foul smell.