12 results about "Penaeus chinensis" patented technology

The invention discloses a net cage device for enhancement and releasing marine temporary rearing of Chinese prawns, the structure of the net cage device comprises a net cage, a dismounting plate, a rope and a floating ball, the dismounting plate is arranged at the left end of the net cage, the outer surface of the net cage is connected with one end of the rope, and the floating ball is fixed at the other end of the rope. Placing frames are installed on the two sides of the interior of a clamping frame in a sliding mode, then the clamping frame extends into the net cage through a dismounting and mounting plate, prawns are located in the net cage, flowing of water can be ensured through a breathable net and a filter screen plate, meanwhile, sundries are effectively blocked, and the prawns in the placing frames are prevented from being affected by the sundries; through cooperation of the water inlet and the slow flow pipe, seawater forms a slow flow state when flowing into the net cage from the outer side of the water inlet, normal flowing of the seawater in the net cage is ensured, and meanwhile the situation that prawns in the containing frame are injured or dead and flushed out due to impact of seawater with too large flow and too high flow speed is avoided.

The invention discloses a method for collecting and dyeing sperms of Chinese prawns, and belongs to the technical field of germplasm resource protection and utilization. The method comprises the following steps: grinding Penaeus chinensis spermatophore with outer membrane removed in a glass homogenizer, adding sterilized artificial seawater, grinding until the solution becomes milky white, and filtering to obtain sperm; and mixing the sperm with eosin-nigrosine dye liquor, and standing to obtain the dyed Chinese prawn sperm. The collecting and dyeing steps are simple and easy to operate, the whole process is short in time consumption, small in sperm damage, visual in effect and high in sperm survival rate. The staining method disclosed by the invention is not only suitable for staining fresh sperms, but also can be used for staining the thawed and resuscitated sperms after cryopreservation, the staining observation after thawing is clear, and the sperms are not easy to crystallize.

The invention relates to a penaeus chinensis 40K liquid phase chip, a probe combination and application thereof, and belongs to the technical field of aquatic molecular breeding biology. The liquid-phase chip comprises probes corresponding to 40 and 803 SNP (Single Nucleotide Polymorphism) loci, and the loci are uniformly distributed on a reference genome ASM1920278v2, are comprehensive in coverage and good in polymorphism, and are particularly closely related to key characters such as growth, stress resistance and disease resistance. Various application requirements of molecular identification, genome selection, functional gene research and the like of the Chinese prawns can be met at the same time, and the method can be used for germplasm resource evaluation and variety identification, population genetic structure research and protection, genome selective breeding, whole genome association analysis, candidate gene mining and the like.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-5C8, and the monoclonal antibody for resisting haemocyanin of Chinese prawns can be expressed through recombinant Expi293 cells of recombinant plasmids pcDNA3.1 containing genes of the monoclonal antibody for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The invention discloses a method for realizing geographical tracing of prawns by analyzing and identifying intestinal microbial flora of prawns on the basis of mass spectrometry and bacterial library search, which is used for directly analyzing and identifying the intestinal microbial flora of aquatic food. The effectiveness of searching and identifying bacteria by using a Bacteria MS database is evaluated and proved, prawns are used as an aquatic food model, and the intestinal microflora of the Chinese prawns collected from three different aquaculture farms in China is analyzed by using a matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The reliability of the method in the aspect of tracking the geographic source of the aquatic product is further verified by analyzing the samples of the black tiger shrimps and the three crucian carps from three different producing areas, and the rapid, accurate and feasible method can be provided for determining the geographic source of the prawn by analyzing the intestinal microflora of the prawn by utilizing MALDI-TOF MS and combining bacterial library search.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-6D6 and can be expressed through a recombinant Expi293 cell of a recombinant plasmid pcDNA3.1 containing a monoclonal antibody gene for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-14B3, and the monoclonal antibody for resisting haemocyanin of Chinese prawns can be expressed through recombinant Expi293 cells of recombinant plasmids pcDNA3.1 containing genes of the monoclonal antibody for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-14B3, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits of Chinese shrimp hemocyanin with molecular weights of 73 kDa and 75 kDa, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-1A1, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-6D6, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-1A1, and the monoclonal antibody for resisting haemocyanin of Chinese prawns can be expressed through recombinant Expi293 cells of recombinant plasmids pcDNA3.1 containing genes of the monoclonal antibody for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.