The invention discloses a Yixingrui Brand soft capsule containing coenzyme Q10 which can improve immunity and fight against cancers, and a preparation method and application thereof. The preparation method comprises the following steps: using 45-55g of raw medicine containing coenzyme Q10 with content more than or equal to 99 percent as an effective ingredient, using 100-300g of perilla herb oil as solvent, preparing the effective ingredient and the solvent into contents at 40-50 DEG C, proportioning 200-300g of gelatin, 100-250g of glycerin, 250-400g of purified water, 0.8-1.2g of pigment and 1-1.2g of pulpy antholeucin to prepare capsule material, adopting sol to prepare the contents and pressing pellets, conducting processes such as drying, pellet picking and the like of a soft capsule preparation technology, and pressing the material medicine into 1000 Yixingrui Brand soft capsules at 50-55 DEG C through a soft capsule pressing technology. The invention is characterized in that the physical efficacy of the coenzyme Q10 is adopted, solvent, vitamin and minerals with collaborative function are screened and the raw medicine is pressed into pellets by adopting the matured Yixingrui Brand soft capsule preparation technology. According to stability tests, evaluative animal safety tests, hygienic tests and efficacy tests, the invention has the advantages of stable form and efficacy ingredients, no toxic and side effects, strong immunologic function, novel anticancer indication effect and promising application prospect.