37results about How to "Avoid experimental failure" patented technology

The invention relates to a method for separating out and purifying cells, in particular to a method for separating out and purifying Kupffer cells in livers, and belongs to the technical field of medicine. By means of the method, Kupffer cells in livers are successfully separated out through mouse liver mechanical circulation pouring and immunomagnetic beads for the first time, the Kupffer cells obtained through the method are high in purity, good in activity, easy to operate and high in generalizability, the specific F4 / 80+CD11c- phenotype is achieved, and stronger macrophage characteristics are achieved. By means of the successful establishment of the cell sorting technology, a new experimental method is provided for further function research on Kupffer cells.

The invention discloses a PCR pre-packing reagent. The PCR pre-packing reagent is a lyophilized reagent, the lyophilized reagent is composed of a PCR reaction mixed solution and a cryoprotectant, andthe weight of the cryoprotectant is 6.25-30% (w / v) of the volume of the PCR reaction mixed solution. The PCR pre-packing reagent simplifies the PCR operation step and improves the experimental efficiency; and the PCR pre-packing reagent also has a high amplification activity and a high quality stability, and can be stored at room temperature for a long term to reduce the storage and transportationcost of the reagent. The invention also provides a reaction tube pre-loaded with the PCR reagent, the reaction chamber of the reaction tube is filled with the PCR pre-packing reagent, an identification code for distinguishing different reaction tubes is arranged on the surface of the reaction tube, and the identification code does not block lights transmitting out of the reaction tube from the reaction chamber. Distinguishing of the different reaction tubes with the identification code is beneficial to achieve automatic matching of PCR detection results with sample and index information, so the automatic operation of PCR detection experiments is realized.

The invention discloses a field sampling reagent storage box. The field sampling reagent storage box comprises a box body, a cover plate and an inner box, the inner wall of the box body is fixedly connected with a shockproof device, the shockproof device comprises a square rubber ring, a fixing plate and a buffer plate, one side of the square rubber ring is fixedly connected with the inner wall ofthe box body, the other side of the square rubber ring is in contact with the surface of the inner box, the top and bottom, which are far away from one side of the inner box, of the buffer plate areboth fixedly connected with buffer springs. The field sampling reagent storage box relates to the technical field of reagent storage boxes. According to the field sampling reagent storage box, after samples are collected in the field, the fact that enough dry ice can be put into a gap between the inner box and the box body is realized, so that transportation is carried out, the effectiveness of areagent is ensured; and at the same time, the shockproof device is arranged, so that the collision between the box body and the outside is avoided, the problem of the failure of a test caused by the rupture of a test tube is further avoid, a good role in protecting the test tube is played, and a foundation for the normal use of the subsequent reagent is laid.

The present invention provides a sterile operation device for stem cell preparation, comprising: a box body comprising at least three operation areas which are independent from each other and are connected with each other by a sterile transfer device; a chemical sterilization device which is communicated with the operation areas; and a physical sterilization device which is connected with the boxbody. According to the sterile operation device for stem cell preparation, through cooperative use of the chemical sterilization device and the physical sterilization device, dead-corner-free sterilization can be effectively achieved, a sterile transfer device is arranged, so that sterile transfer can be achieved in multiple links of stem cell preparation, and experiment failures caused by bacterial contamination in the transfer process are avoided.

The invention discloses a composite fulvic acid antibacterial sponge matrix and a preparation method and application thereof, belonging to the technical field of material preparation and environmentalgovernance. The preparation method comprises the following steps: polymerizing fulvic acid, acrylic acid and antibacterial natural macromolecules under the conditions of an initiator and a cross-linking agent to obtain a fulvic acid water-retaining agent, polymerizing polytetrahydrofuran with isocyanate, N-methylpyrrolidone, a catalyst, a cross-linking agent and the like to form a polyurethane emulsion, and compounding the polyurethane emulsion with the fulvic acid water-retaining agent to obtain the composite fulvic acid antibacterial sponge matrix. Components contained in the water-retaining fulvic acid composite antibacterial sponge matrix are environment-friendly materials and are non-toxic; and the porous structure of the sponge matrix and a large number of hydrophilic groups such asacrylic acid and polyurethane endow the sponge matrix with good water storage capacity, and rainwater can be effectively stored in the sponge matrix and purified. Antibacterial macromolecules are added to prevent the sponge from mildewing, root rotting and the like due to long-term contact with water, so the sponge matrix can be widely applied as a soilless culture substrate in sponge city construction.

The invention relates to a liquid viscosity coefficient measuring device and a working method. The liquid viscosity coefficient measuring device comprises a liquid accommodating device, wherein a cross beam is arranged above the liquid accommodating device; an aligning mechanism is fixedly arranged on the cross beam; the aligning mechanism is positioned over the liquid accommodating device, and is used for adjusting a small ball used for experiments to fall off along the vertical direction; a support is arranged on one side of the aligning mechanism; the support is connected with an electromagnet through an adjusting mechanism; the adjusting mechanism can adjust the positions of the electromagnet in the vertical direction and the vertical direction; the electromagnet is used for adsorbing the small ball used for experiments; a picture recording device support is arranged on one side of the liquid accommodating device; a lifting mechanism is arranged on the picture recording device support; the lifting mechanism is connected with a picture recording device; the picture recording device is used for recording pictures in the falling process of the small ball. The measuring device is convenient to use, high in measuring efficiency and accurate in measuring results.

The invention relates to a temperature controllable type multifunctional animal spinal cord injury impactor. The impactor comprises a fixer platform, wherein the fixer platform is provided with a size adjustable fixing collar and multiple sliding grooves, limb fixing rings are installed on the sliding grooves, a vertical rod with scale marks is detachably installed at one end of the fixer platform and located in front of the fixing collar, the vertical rod is sleeved with a connecting arm with scale marks, the connecting arm can rotate around the vertical rod while sliding up and down along the vertical rod, a hollow pipe is movably connected to the connecting arm and slides leftwards and rightwards along the connecting arm, a heating device provided with a temperature controller inside is installed on the bottom of the fixer platform, and a plug is connected to the heating device. By the adoption of the impactor, experiment failures caused by evasion of animals are effectively avoided, and experiment efficiency and experiment precision are guaranteed. The impactor is simple in structure, easy to operate and worth application and popularization.

The invention discloses a composite humic acid sponge bionic soil matrix and a preparation method and application thereof, belonging to the technical field of material preparation and environmental governance. The preparation method comprises the following steps: mixing polyether polyol and N-methyl pyrrolidone, carrying out stirring in a water bath under the action of a catalyst, a cross-linkingagent and a chain extender, dropwise adding isocyanate to obtain a polyurethane emulsion, and compounding humic acid with the polyurethane emulsion in the foaming stage of polyurethane sponge to obtain the composite humic acid sponge bionic soil matrix. The composite humic acid sponge bionic soil matrix has the same growth effect on plants and the same complexing adsorption effect on pollutant ions as humic acid, and the same portable water absorption effect as the sponge. The method is simple and convenient to operate, uses easily available raw materials, and has low equipment requirements and low cost. The prepared composite humic acid sponge bionic soil matrix is light in weight, uniform in holes, high in water absorption and retention rate, capable of effectively promoting plant growthand purifying water, safe and friendly to environment.

The invention discloses an automatic liquid nitrogen control and supplementing device of a high-temperature superconductor strip mechanics experiment machine and a method. The main composition of thedevice is as follows: a vertical strip driving mechanism of the high-temperature superconductor strip mechanics experiment machine is placed on a top plate, an open dewar flask is placed on a lower bottom plate and located right below the vertical strip driving mechanism, and a closed liquid supplementing dewar flask is also arranged on the lower bottom plate; a polytetrafluoroethylene rod is fixed on the side surface of the dewar flask, and a superconductor liquid level meter is fixed on the inner side of the polytetrafluoroethylene rod and not contacted with the inner wall of the dewar flask; the superconductor liquid level meter is connected to a liquid level controller, meanwhile, the liquid level controller is connected with a control end of a pressurizing valve, and the pressurizingvalve is fixed at the top of the liquid supplementing dewar flask, and the valve opening extends into the liquid supplementing dewar flask. The device can automatically ensure that liquid nitrogen inthe dewar flask always flood a high-temperature superconductor strip, time and labor are saved during experiments, the experiment efficiency is high, the experiment success rate is high, and experiment results are reliable.

The invention relates to the field of smart laboratories and provides a multipurpose experiment desk for a smart laboratory. The multipurpose experiment desk comprises a supporting table, a pluralityof storage cabinets are arranged on the front side of the supporting table, a base is arranged at the lower end of the supporting tables, an experiment table is arranged on the upper side of the supporting table, an accommodating cavity is formed in the upper side of the experiment table, lifting cavities are formed in the accommodating cavity in a bilateral symmetry mode, a driving motor is arranged at the lower end in the lifting cavity, a lifting threaded rod is arranged at the output end of the driving motor, lifting seats are in threaded connection with the lifting threaded rod, an experiment table top is arranged between the two lifting seats, mounting frames are arranged at the upper end of the experiment desk in a bilateral symmetry mode, sealing doors are mounted on the mounting frames through rotating shafts, and a fingerprint lock is arranged at the joint of the two sealing doors. Due to the arrangement of the structure, when people leave in the midway of an experiment, experiment failure caused by accidental touch of external personnel can be prevented, confidential leakage can also be prevented, and use comfort and safety are improved.

The invention discloses an experimental animal disinfection treatment device. The device includes a box body. The box body has an entrance and an exit. The bottom of the box body is provided with a main conveyor belt. Sun blinds are respectively arranged at the entrance and the exit. The box body is provided with a liquid storage tank and multiple atomizing nozzles communicated with the liquid storage tank through a pipe. The pipe is provided with a pump for conveying the liquid in the liquid storage tank into the atomizing nozzles. The device satisfies the requirements of sterilizing and disinfecting livestock and poultries and reduces the labor load of the animal experimenter.

The invention discloses a magnetic stirring water bath kettle provided with a support. The magnetic stirring water bath kettle comprises a water bath kettle body (1) and is characterized in that the water bath kettle body (1) comprises a water tank layer (2) located on the upper layer and a driving layer (3) located on the lower layer, the water tank layer (2) and the driving layer (3) are separated by a baffle (4), the water tank layer (2) is provided with the support (5), and the driving layer (3) is provided with a magnetic stirring driving system (6) located right below the support (5). The magnetic stirring water bath kettle provided with the support is simple in structure, reasonable in design, capable of realizing operations such as heating of a large container and a small container and the like simultaneously and stirring a test solution, and suitable for being widely popularized and applied to laboratories.

The invention discloses a biochemical product syringe used for rodent experiments, and specifically relates to the technical field of medical instruments. The biochemical product syringe used for therodent experiments comprises an arcuate fixing plate; notches are arranged at middle parts of the bottom on two sides of the arcuate fixing plate in a penetrating way; first chutes are arranged, on two sides of the arcuate fixing plate, at the tops of the notches; first sliders are arranged at the bottoms of the notches; fixing holes are arranged at the bottom ends of the first sliders in a penetrating way; a through groove is arranged at a central position of the top of the arcuate fixing plate; and second chutes are arranged at middle parts of the inner walls on two sides of the through groove. According to the biochemical product syringe used for the rodent experiments, four limbs of rodents of various sizes can be fixed by utilizing the first sliders on the arcuate fixing plate. The trunk part of an experimental rodent can be fixed by utilizing an inflated airbag; sleeves are twisted so as to adjust distances between the top end of the sleeves and the bottom end of a second slider;and sleeve rings are twisted so as to adjust relative height of the sleeve rings on the syringe, thereby realizing adjustment on needle insertion depth.

The invention discloses a modular sabot separation device. The modular sabot separation device comprises a fastener, a front fixator, a sabot separator and a first-stage fragment recoverer, wherein through chambers are formed in the front fixator, the sabot separator and the first-stage fragment recoverer, and the two ends of the sabot separator are coaxially connected with one end of the front fixator and one end of the first-stage fragment recoverer respectively; and the other end of the front fixator is connected with a bulkhead of a target chamber through the fastener. The invention also discloses a using method of the modular sabot separation device. When the device is used for an impact test, the original device structure does not need to be changed. The device can be independently used for all test equipment. Fragments generated by a sabot and collision can be effectively separated and recycled, the protection effect on experimenters is achieved, and the safety performance is improved. The influence of followers produced by the sabot after collision on an experimental result can be effectively eliminated. The modular sabot separation device is coaxial with a gun barrel, andthe diameters of two separation holes are larger than the diameter of a projectile and smaller than the diameter of the sabot. A ballistic trajectory can be corrected, and experiment failures caused by ballistic trajectory deviation are avoided.

The invention discloses a device and method for sensitively detecting a film penetration pressure value in a film distillation process, wherein the device comprises a gas storage tank, a film penetration test assembly, a direct current power supply, a high-sensitivity electric bridge and a diaphragm to be tested, wherein the film penetration test assembly comprises an accommodating barrel, a flange plate and a bottom plate; the diaphragm to be tested is positioned between the flange plate and the bottom plate; a conducting sheet is arranged between the diaphragm to be tested and the bottom plate; the gas storage tank is connected with the accommodating barrel through a pressure reducing valve, a pressure regulator and a pressure meter; an upper binding post and a lower binding post are further arranged on the upper end of the accommodating barrel and the lower side of the conducting sheet; the direct current power supply is connected with the upper binding post and the lower binding post; two conducting posts are further distributed on the side wall of the accommodating barrel; and the high-sensitivity electric bridge is connected with the two conducting posts. By using the method,the device is used for performing film penetration pressure detection. According to the device and the method provided by the invention, the film soaking phenomenon can be reflected through the electric parameter detection of the film assembly, so that the maximum operation pressure capable of being born by the system can be fast and accurately indicated.

The invention discloses an ntCre / LoxP deletion system controlled by heat shock and tetracycline. The ntCre / LoxP deletion system comprises 2 LoxP loci specifically identified by ntCre recombinase and a target gene part positioned between the 2 LoxP loci, wherein the 2 LoxP loci are respectively a first LoxP locus positioned at the 3' end of target gene and a second LoxP locus positioned at the 5' end of the target gene; and the ntCre / LoxP deletion system also comprises an Rhsf gene expression box controlled by an HSF70m promoter and an ntCre recombinase gene expression box part controlled by an Om35S promoter, wherein the Om35S promoter is controlled by Rhsf gene. The deletion system is controlled by the heat shock and the tetracycline, and the target gene between the 2 LoxP loci can be deleted through heat shock under the condition of no tetracycline. The invention also discloses a recombinant expression vector containing the ntCre / LoxP deletion system. The recombinant expression vector can be applied to deleting target genes in plant transgenes, and has the advantages of short deletion period, simple operation and the like.

The invention discloses an animal fixation box. The animal fixation box comprises a rectangular frame which is formed by a front wall, two side walls (1) and a rear wall (2) in an enclosing mode and provided with an upper opening and a lower opening; a box bottom (3) is arranged at the lower opening of the rectangular frame, and a box cover (4) is arranged at the upper opening in a covering mode; the front wall is divided into an upper front wall (51) and a lower front wall (52), the upper edge of the lower front wall (52) and the lower edge of the upper front wall (51) are each provided with a semicircular notch, and the two semicircular notches can be matched with each other and locked through lock catches (8) to form a hole (6) used for fixing an animal to be fixed; the diameter of the hole (6) is larger than that of the neck of the animal to be fixed and smaller than that of the head of the animal to be fixed; one end of the box cover (4) is hinged to the top end of the rear wall (2), and the other end of the box cover (4) is connected with the upper edge of the upper front wall (51). The animal fixation box is wide in application range, fixation operation is easy and convenient, and the animal can be carried and moved with the animal fixation box conveniently.

The invention discloses a composite humic acid sponge bionic soil matrix, a preparation method and application thereof, and belongs to the technical field of material preparation and environmental treatment. After mixing polyether polyol and N-methylpyrrolidone, under the action of catalyst, cross-linking agent and chain extender, after stirring in a water bath, isocyanate is added dropwise to obtain polyurethane emulsion. In the foaming stage of polyurethane sponge, humic acid and The composite humic acid sponge biomimetic soil matrix is ​​obtained. It has both the growth effect of humic acid on plants, the complexation and adsorption effect on pollutant ions, and the light water absorption effect of sponge. The method has the advantages of simple operation, readily available raw materials, low equipment requirements and low cost. The prepared product is light in weight, uniform in pores, high in water absorption and water retention rate, can effectively promote plant growth, purify water quality, and is safe and environmentally friendly.

The invention discloses an antiskid beaker, and relates to a biological and chemical experimental appliance. The structure of the antiskid beaker is that antiskid surfaces are processed on two side walls of a beaker body; the two antiskid surfaces are symmetrically processed on the wall of the beaker body; the antiskid surfaces are concave surfaces towards the wall of the beaker body; and frosted surfaces or antiskid bumps are processed on the antiskid surfaces. The antiskid beaker has the characteristics of being novel in structure, simple and convenient to process, convenient to use, safe, reliable, economical and practical, and is a novel antiskid beaker integrating the economical efficiency and the practicability.

The invention discloses a thin-wall sample test fixture, and relates to the related technical field of thin-wall sample test. Comprising an upper chuck, a lower chuck and a cushion block, a threaded head is arranged at one end of the upper chuck, a square hole is formed in the side wall of the other end of the upper chuck, a square groove communicated with the square hole is formed in the side wall of the end, away from the threaded head, of the square hole, one side of the square groove is open, and the other side of the square groove is closed. The strip-shaped hole penetrates through the square groove and the closed side of the square groove and then is communicated with the square hole, and radial projections of the square hole and the square groove are symmetrically located on the two sides of the radial projection of the strip-shaped hole respectively. The lower chuck and the upper chuck have the same structure; the cushion blocks are used for fixedly abutting the two ends of the thin-wall sample penetrating through the strip-shaped holes into the square grooves of the upper chuck and the lower chuck respectively. According to the thin-wall sample test fixture provided by the invention, the thin-wall sample to be processed can be fixed at the axis of the chuck, so that the experiment accuracy is greatly improved.

The invention belongs to chemical machinery and provides a dispersion machine with a time accumulation metering device. The dispersion machine comprises a rack, wherein the top surface of the rack is provided with a switch which is electrically connected with an electric supply; the time accumulation metering device with a liquid crystal screen is embedded on the rack in a welding manner; the rack is internally provided with a power supply circuit; and the time accumulation metering device is electrically connected with the switch by the power supply circuit. In dispersion operation, the time accumulation metering device can automatically record time and times when the switch is switched on, and the defect that an existing dispersion machine cannot time and meter is overcome; and experiment failures caused by the fact that people forget to check or record are avoided and an operation platform keeps clean and tidy.

The invention discloses a device and method for sensitively detecting membrane permeation pressure value in membrane distillation process, wherein, the device includes a gas storage tank, a membrane permeation test component, a DC power supply, a high-sensitivity electric bridge and a diaphragm to be tested; the membrane The soaking test assembly includes an accommodating cylinder, a flange plate and a bottom plate, the diaphragm to be tested is located between the flange plate and the bottom plate, and a conductive sheet is arranged between the diaphragm to be tested and the bottom plate; the gas storage tank is reduced The pressure valve, the pressure regulator and the pressure gauge are connected to the storage cylinder; the upper terminal and the lower terminal are also arranged on the upper end of the storage cylinder and the lower side of the conductive sheet, and the DC power supply is connected with the upper terminal and the lower terminal. The connecting posts are connected; two lead posts are distributed on the side wall of the containing cylinder, and the high-sensitivity electric bridge is connected with the two lead posts; the method is to use the above-mentioned device to detect the membrane penetration pressure value. The invention can reflect the membrane wetting phenomenon through the electrical parameter detection of the membrane module, so as to quickly and accurately indicate that the system can bear the maximum operating pressure.

The invention relates to an animal spinal cord injury hitting device which comprises a fixator platform. Fixing rings are arranged at the two ends of the fixator platform, sliding grooves are formed in the fixator platform, limb fixing rings are arranged on the sliding grooves, spine fixing clamping plate assemblies are arranged on the two sides of the fixator platform, vertical rods are arranged at the end of the fixator platform and sleeved with a first scale fixing plate and a second scale fixing plate, a first sliding rail is arranged on the first scale fixing plate, a second sliding rail is arranged on the second scale fixing plate, a first clamping ring is arranged on the first sliding rail, a second clamping ring is arranged on the second sliding rail, an outer casing pipe is inserted into the first clamping ring, the end of the outer casing pipe penetrates through the second clamping ring, an inner casing pipe is arranged in the outer casing pipe, a hitting needle is arranged in the inner casing pipe, and a hitting needle frequency control device is arranged on the outer casing pipe, and connected with the hitting needle. Experiment failures caused by animal hiding are effectively avoided, experiment efficiency and experiment precision are guaranteed, the structure is simple, operation is easy, and the hitting device is worthy of application and popularization.

The invention relates to the technical field of control and regulation, and provides a reaction kettle control valve capable of realizing automatic flow control through a time delay structure. The reaction kettle control valve comprises a reaction kettle control valve body, the upper side and the lower side of the reaction kettle control valve body are fixedly connected with thermometers, the upper side and the lower side of the reaction kettle control valve body are movably connected with swing control valves through the thermometers, and the left side and the right side of the reaction kettle control valve body are movably connected with movable air bags through the swing control valves. According to the reaction kettle control valve capable of realizing automatic flow control through the time delay structure, when a reaction kettle begins to operate, the reaction kettle conducts feeding according to different stages of temperature rising, the temperature rises to 30 DEG C accordingto the principle of thermal expansion and cold contraction, a temperature sensing pipe begins to expand to extrude internal airflow, the airflow pushes a connecting pressing rod to extrude a telescopic spring rod, the airflow enters an air pipe, a movable telescopic frame is pushed through the air pipe, and the movable telescopic frame pushes the swing control valves to be pushed to the 30-degreeareas of the thermometers through inclined blocks.

The invention discloses an animal fixation box. The animal fixation box comprises a rectangular frame which is formed by a front wall, two side walls (1) and a rear wall (2) in an enclosing mode and provided with an upper opening and a lower opening; a box bottom (3) is arranged at the lower opening of the rectangular frame, and a box cover (4) is arranged at the upper opening in a covering mode; the front wall is divided into an upper front wall (51) and a lower front wall (52), the upper edge of the lower front wall (52) and the lower edge of the upper front wall (51) are each provided with a semicircular notch, and the two semicircular notches can be matched with each other and locked through lock catches (8) to form a hole (6) used for fixing an animal to be fixed; the diameter of the hole (6) is larger than that of the neck of the animal to be fixed and smaller than that of the head of the animal to be fixed; one end of the box cover (4) is hinged to the top end of the rear wall (2), and the other end of the box cover (4) is connected with the upper edge of the upper front wall (51). The animal fixation box is wide in application range, fixation operation is easy and convenient, and the animal can be carried and moved with the animal fixation box conveniently.

The present invention discloses a mechanical teaching aid convenient for the students to demonstrate. The mechanical teaching aid comprises two supporting plates, the tops of the supporting plates are fixedly connected with the supporting legs, and the tops of the supporting legs are fixedly connected with a connecting casing. The bottom of the connecting casing is movably connected with a U-shaped block, an inner cavity of the U-shaped block is connected with a pulley movably via a rotating shaft, the surface of the pulley is movably connected with a traction belt, and the two ends of the traction belt both extend to the bottom of the pulley. According to the present invention, by rotating a rotating handle to drive a rotating rod and a gear to rotate, and by the rotation of the gear, a toothed plate can be moved rightwards to an inner cavity of a fastening groove, and then a pulley block demonstration test can be operated. After an experiment is ended, by rotating the rotating handle reversely, the toothed plate can be taken out from the inner cavity of the fastening groove. The experiment process is not limited by the places, the operations are very convenient, the interests of the students are enhanced, and naturally, the academic records of the students are improved.

The invention discloses a biochemical product syringe used for rodent experiments, and specifically relates to the technical field of medical instruments. The biochemical product syringe used for therodent experiments comprises an arcuate fixing plate; notches are arranged at middle parts of the bottom on two sides of the arcuate fixing plate in a penetrating way; first chutes are arranged, on two sides of the arcuate fixing plate, at the tops of the notches; first sliders are arranged at the bottoms of the notches; fixing holes are arranged at the bottom ends of the first sliders in a penetrating way; a through groove is arranged at a central position of the top of the arcuate fixing plate; and second chutes are arranged at middle parts of the inner walls on two sides of the through groove. According to the biochemical product syringe used for the rodent experiments, four limbs of rodents of various sizes can be fixed by utilizing the first sliders on the arcuate fixing plate. The trunk part of an experimental rodent can be fixed by utilizing an inflated airbag; sleeves are twisted so as to adjust distances between the top end of the sleeves and the bottom end of a second slider;and sleeve rings are twisted so as to adjust relative height of the sleeve rings on the syringe, thereby realizing adjustment on needle insertion depth.

The invention provides a method for evaluating the nicotine addiction degree or evaluating the influence of a to-be-detected medicine on the nicotine addiction degree, which comprises the following steps: S1) providing a nicotine-containing aqueous solution as the only water source of a test animal, feeding and recording the times of licking the nicotine-containing aqueous solution by the test animal, when the frequency of licking the nicotine-containing water solution by the test animal is stable, obtaining a nicotine addiction test animal model; s2) replacing a water source with a nicotine-free aqueous solution to obtain a test animal model generating nicotine withdrawal; and S3) evaluating the nicotine addiction degree by evaluating the water source licking frequency or frequency of the experimental animal generating nicotine withdrawal, or evaluating the influence of a to-be-detected medicine on the nicotine addiction degree by the water source licking frequency or frequency of the experimental animal after the to-be-detected medicine is given to the experimental animal. The preparation method of the animal model is very simple, does not need surgical procedures, and can quantitatively evaluate the addiction and withdrawal degrees of experimental animals.

The invention relates to a constant-temperature reaction container and a constant-temperature method, belonging to the technical field of reactors. The constant-temperature reaction container comprises a magnetic stirrer, a constant-temperature liquid bath pot, a combined lantern ring component, a temperature sensor and a reactor, wherein the constant-temperature liquid bath pot is fixedly arranged in the center of the top end of the magnetic stirrer; the bottom end of the reactor is arranged on the magnetic surface of the top end of the magnetic stirrer; the bottom of the reactor is arrangedin the constant-temperature liquid bath pot; the combined lantern ring component is arranged at the top of the constant-temperature liquid bath pot; a positioning piece is arranged on the combined lantern ring component; the top of the constant-temperature liquid bath pot reactor is fixedly arranged on the combined lantern ring component through the positioning piece; and the temperature sensor penetrates through the combined lantern ring component and extends into the reactor. According to the invention, the temperature in the reactor is monitored in real time through the temperature sensor,and constant temperature is achieved; and positioning and stabilization of the reactor are achieved through the positioning piece of the combined lantern ring component, and experiment failure causedby tipping of the reaction container is prevented.

The invention provides a membrane material fixing buckle suitable for a cell culture board. The membrane material fixing buckle is composed of a snap ring and a snap ring base. The snap ring base hasa Chinese character Tu-shaped structure and is provided with a through hole from top to bottom. The snap ring sleeves the upper part of the snap ring base and is extruded and meshed and the connectionmanner is interference fit connection. The outer diameter of the membrane material fixing buckle is set according to the diameter of the culture hole of the different types of cell culture boards, and is smaller than the culture hole diameter of the corresponding type of a cell culture board. The membrane material fixing buckle solves the problems of membrane material wrinkles and tight fitting of the membrane material and culture hole bottom and prevents that cells penetrate into the bottom of the membrane so that cells do not grow on the membrane to cause experiment failure. The membrane material is clamped and sheared and then is put into a culture hole of the cell culture board so that an operation process is finished and is simple and convenient. The membrane material fixing buckle is prepared from a polytetrafluoroethylene material, has the advantages of high temperature and low temperature resistance, chemical corrosion resistance and no adhesion to any substance, and does notproduce influence on cell culture.