The invention relates to a disc flower flocked antibacterial and crease-resistant lining provided with an all cotton fabric layer. The lining comprises a lining body (1), wherein the lining body (1) is reticular, a disc flower layer (2) is arranged on the upper surface of the lining body (1), a flocked layer (3) is arranged on the lower surface of the lining body (1), disc flowers are arranged on the disc flower layer (2), velvet flowers are arranged on the flocked layer (3), the lining body (1) is made from a fabric, the fabric is composed of a front crease-resistant layer, an intermediate adhesive layer and a back antibacterial layer, the crease-resistant layer is made from a polyurethane material with memory function, the adhesive layer is of a thermosetting type, and the antibacterial layer is a fabric layer containing nano bamboo carbon fibers. Raised disc flowers and velvet flowers enhance sense of depth of the disc flower flocked antibacterial and crease-resistant lining provided with the all cotton fabric layer, and handfeel is soft and comfortable. The lining provided by the invention is antibacterial and crease-resistant.