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Thioredoxin gene of Schistosoma japonicum (Chinese Mainland Strain) and its cloning expression method and application

A technology for straining thioredoxin and schistosomiasis, applied in application, gene therapy, genetic engineering, etc., can solve problems that have not yet been seen

Inactive Publication Date: 2007-09-05
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports related to the research on the thioredoxin (SjcTrx) gene and its application of Schistosoma japonicum mainland China strain in this manual

Method used

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  • Thioredoxin gene of Schistosoma japonicum (Chinese Mainland Strain) and its cloning expression method and application
  • Thioredoxin gene of Schistosoma japonicum (Chinese Mainland Strain) and its cloning expression method and application
  • Thioredoxin gene of Schistosoma japonicum (Chinese Mainland Strain) and its cloning expression method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Cloning and expression of Schistosoma japonicum thioredoxin gene and immune protection test of recombinant protein mice

[0026] 1. Materials

[0027] 1.1 The cercariae and adults of the continental strain of Schistosoma japonicum: the positive snails containing mature cercariae were provided by the key laboratory of the Institute, and the cercariae were escaped by conventional methods. The adult worms (mixed male and female) of the continental strain of Schistosoma japonicum were obtained from rabbits infected with cercariae of Schistosoma japonicum for 6 weeks.

[0028] 1.2 Strains and plasmids: Escherichia coli BL21 strain and vector pET28a were purchased from Novagen.

[0029] 1.3 Experimental animals: 6 weeks old C 57 BL / 6 female mice were provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences.

[0030] 1.4 Main reagents: total RNA extraction kit, plasmid DNA extraction kit, PCR product purification kit, enzyme digestion product p...

Embodiment 2

[0080] Example 2, Construction of Schistosoma japonicum Thioredoxin Nucleic Acid Vaccine and Experiment of Nucleic Acid Vaccine Mice Immune Protection

[0081] 1 material

[0082] 1.1 The cercariae and adults of the continental strain of Schistosoma japonicum: the positive snails containing mature cercariae were provided by the key laboratory of the Institute, and the cercariae were escaped by conventional methods. The adult worms (mixed male and female) of the continental strain of Schistosoma japonicum were obtained from rabbits infected with cercariae of Schistosoma japonicum for 6 weeks.

[0083] 1.2 Strains and plasmids: Escherichia coli JM109 strain was purchased from Invotrogen Company and the vector pcDNA3 was purchased from Novagen Company.

[0084] 1.3 Experimental animals: 6 weeks old C 57 BL / 6 female mice were provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences.

[0085] 1.4 Main reagents: total RNA extraction kit, plasmid DNA extraction...

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Abstract

The present invention relates to Schistosomiasis japonica Chinese Mainland strain thioredoxin (SjcTrx) gene sequence and its cloning expression method and application. The SjcTrx gene of the present invention has the nucleotide sequence shown in sequence list 1 and codes corresponding amino acid sequence. The present invention also discloses the expression method of the SjcTrx gene in colibacillus. By means of preparing purified recombinant protein an DNA vaccine and immunizing C57BL / 6 mouse, it is found that the SjcTrx gene of the present invention may be used in preparing recombinant protein vaccine and DNA vaccine for preventing and treating Schistosomiasis japonica.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to the sequence of the thioredoxin (SjcTrx) gene of Schistosoma japonicum (Chinese mainland strain) and its cloning and expression method and application. Background technique [0002] Schistosomiasis japonicum was once widely prevalent in the Yangtze River Basin and 12 provinces, municipalities, and autonomous regions to the south of my country. In the early 1950s, there were about 11.6 million schistosomiasis patients nationwide, and the distribution area of ​​the intermediate host snails was 14.8 billion m 2 . After more than half a century of unremitting efforts, the prevention and control of schistosomiasis in my country has achieved world-renowned achievements. By 1995, 5 provinces (autonomous regions and municipalities) including Guangdong, Shanghai, Fujian, Guangxi, and Zhejiang had reached the prevention and control of schistosomiasis transmission. break the...

Claims

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Application Information

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IPC IPC(8): C12N15/30C07K14/44C12N15/10C12N15/70A61K39/002A61K48/00
CPCY02A50/30
Inventor 曹建平韩海勃刘述先徐馀信汤林华沈玉娟李小红卢潍媛
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT