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Use of cepharanthine for preparing HSV-II drug

A technology of herpes simplex virus and stepahin, which is applied in antiviral agents, drug delivery, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2007-11-07
GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antiviral drugs with specific efficacy against these two viruses are currently lacking
Moreover, due to the difference between type I and type II, the research on antiviral drugs must also be screened and tested separately

Method used

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  • Use of cepharanthine for preparing HSV-II drug
  • Use of cepharanthine for preparing HSV-II drug
  • Use of cepharanthine for preparing HSV-II drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] (1) Materials

[0019] 1. Equipment

[0020] Imported 96-well culture plate (flat bottom), inverted microscope, CO 2 Cell incubator, micro-adjustable sample injector, medium is DMEM (containing blue chain double antibody 250U / ml), pH is adjusted to 7.0-7.2 with sodium bicarbonate before sterilization and filtration, and calf serum is sterilized at 56°C for 30 minutes Live complement, sterilized and aliquoted; the digestive solution is 5% trypsin 5ml+1%EDTA 2ml+0.01mol / L (pH 7.2) PBS 93ml, sterilized and aliquoted, and stored at low temperature.

[0021] 2. Viruses and cell lines:

[0022] The herpes simplex virus type II strain is the HSV-II333 strain from the Institute of Virology of Wuhan University, which is preserved by the applicant's laboratory. Vero cells were purchased from ATCC Company in the United States and kept by the applicant's laboratory. Vero cells were continuously passaged in DMEM medium containing 10% calf serum for 3 times and kept in the logari...

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Abstract

The present invention relates to a new application of cepharanthine, in particular, it relates to application of cepharanthine in preparation of medicine for resisting herpes simplex virus II type. The tests show that the cepharanthine has the action for preventing and curing cell infected with HSV-II, said cepharanthine can reduce infectiousness of HSV-II to Vero cell, and has the action of directly killing HSV-II, so that the cepharanthine can be used for preparing the medicine for resisting HSV-II, specially can be used for preparing the medicine for curing genital herpes.

Description

technical field [0001] The present invention relates to the new use of stepherpelin, in particular to the use of stepherpelin for preparing anti-herpes simplex virus type II medicine. Background technique [0002] Cepharanthine is a bisbenzylisoquinoline alkaloid extracted from Stephaniajaponiaca (Thunb 1) Miers. The role of Stephalin is to promote the proliferation of bone marrow tissue, thereby increasing white blood cells. It is mainly used clinically for leukopenia. In addition, some studies have found that paternogenin can inhibit NF-κ reverse MDR and promote apoptosis of drug-resistant cells. Foreign studies have found that many of these alkaloids have strong anti-inflammatory, anti-viral, anti-hemolytic, and anti-tumor effects, can stimulate the reticuloendothelial system, activate hematopoietic tissue, and promote bone marrow hyperplasia. [0003] Genital herpes, also known as genital herpes, is a sexually transmitted disease caused by herpes simplex virus (Herps ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4745A61K9/08A61K9/14A61K9/20A61K9/48A61P31/22
Inventor 王一飞王庆端杨崇仁
Owner GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT