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Method for preparing molecular engram CS/TEOS hybridized film by separation of phenylalanine isomer

A technology of phenylalanine and molecular imprinting, which is applied in chemical instruments and methods, semipermeable membrane separation, membrane technology, etc., can solve the problems of complex preparation process, reduced separation performance, and small flux, and achieves a simple and convenient preparation method. The effect of improved splitting ability

Inactive Publication Date: 2007-11-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecularly imprinted membranes made of inorganic materials have the advantage of high recognition ability, but they are relatively brittle, the preparation process is complicated, and the range of applicable imprinted molecules is limited.
Organic membrane materials have the advantage of being easy to form a film, but the flux of a membrane with good recognition performance is often too small, while an organic membrane with a large flux often has a large degree of swelling, and the ability to maintain the structure of the recognition site is poor, resulting in poor separation performance. reduce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Preparation of Molecularly Imprinted CS / TEOS Organic-Inorganic Hybrid Membrane (Membrane 1)

[0012] The chitosan (CS) of 1g is joined in the 2wt.% acetic acid aqueous solution of 50ml, under 60 ℃ of water baths, stir until chitosan dissolves, remove insoluble part by filter cloth filtration, obtain the chitosan solution of 2wt.%. Add 0.75g of tetraethyl orthosilicate (TEOS) to the solution under stirring condition, and then add 6ml of 0.1M L-phenylalanine aqueous solution to it after stirring well, continue to stir for 12h, after standing for defoaming, put The casting liquid is poured on a clean plexiglass plate placed horizontally, cast, and after drying at room temperature for 24 hours, the casting liquid is no longer viscous, and a smooth and transparent film is formed on the plexiglass plate. At this time, the film is slowly Peel off from the substrate to obtain a molecularly imprinted CS / TEOS hybrid film. Then the obtained membrane was put into a drying oven for...

Embodiment 2

[0015] Preparation of Molecularly Imprinted CS / TEOS Organic-Inorganic Hybrid Membrane (Membrane 2)

[0016] The chitosan (CS) of 1g is joined in the 2wt.% acetic acid aqueous solution of 50ml, under 60 ℃ of water baths, stir until chitosan dissolves, remove insoluble part by filter cloth filtration, obtain the chitosan solution of 2wt.%. Add 1.5g of tetraethyl orthosilicate (TEOS) to the solution under stirring condition, and then add 6ml of 0.1M L-phenylalanine aqueous solution to it after stirring well, continue to stir for 12h, after standing for defoaming, put The casting liquid is poured on a clean plexiglass plate placed horizontally, cast, and after drying at room temperature for 24 hours, the casting liquid is no longer viscous, and a smooth and transparent film is formed on the plexiglass plate. At this time, the film is slowly Peel off from the substrate to obtain a molecularly imprinted CS / TEOS hybrid film. Then the obtained membrane was put into a drying oven for ...

Embodiment 3

[0019] Preparation of Molecularly Imprinted CS / TEOS Organic-Inorganic Hybrid Membrane (Membrane 3)

[0020] The chitosan (CS) of 1g is joined in the 2wt.% acetic acid aqueous solution of 50ml, under 60 ℃ of water baths, stir until chitosan dissolves, remove insoluble part by filter cloth filtration, obtain the chitosan solution of 2wt.%. Add 2.5g of tetraethyl orthosilicate (TEOS) to the solution under stirring condition, and then add 10ml of 0.1M L-phenylalanine aqueous solution to it after stirring well, continue stirring for 12h, after standing for defoaming, put The casting liquid is poured on a clean plexiglass plate placed horizontally, cast, and after drying at room temperature for 24 hours, the casting liquid is no longer viscous, and a smooth and transparent film is formed on the plexiglass plate. At this time, the film is slowly Peel off from the substrate to obtain a molecularly imprinted CS / TEOS hybrid film. Then the obtained membrane was put into a drying oven fo...

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Abstract

The invention discloses a method for preparing molecule print chitose / silicic acid carbethoxy hybridized film used to separate phenylalanine isomer of water solution. Said method comprises: adding chitose into acetate water solution whose content is 2%; mixing and filtering insoluble part with filter cloth to attain chitose solution; mixing and adding silicic acid carbethoxy into chitose solution; fully mixing, than adding L-phenylalanine water solution to be mixed for 12 hours; defoaming and filling casting liquid on the horizontally arranged clear organic glass plate horizontally to be extended and form transparent film; thermal treating the film and washing. The invention has the advantages that: the method is simple; attained molecule print chitose / silicic acid carbethoxy film has high density without significant phase separation, and it has better mechanical strength, and better separation function on phenylalanine isomer.

Description

technical field [0001] The invention relates to a method for preparing a molecularly imprinted CS / TEOS hybrid membrane for separating phenylalanine isomers (D, L-Phe). It belongs to the membrane technology of organic-inorganic hybrid membrane. Background technique [0002] Chirality is a common feature of nature. Macromolecules such as proteins, polysaccharides, and nucleic acids that constitute the basis of life activities are almost all chiral. There are also a large number of chiral substances in artificially synthesized products such as medicines. Their physical and chemical properties are similar, but However, the biological activity is often very different. Therefore, the resolution of chiral substances is of great significance. Since the molecular formulas of chiral substances are exactly the same and their physical and chemical properties are extremely similar, the separation of chiral substances is very difficult. The resolution of chiral ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D71/08B01D71/70
Inventor 吴洪姜忠义喻应霞赵艳艳
Owner TIANJIN UNIV