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Microbe bacteria agent for treatment of refinery waste water, its preparation method and application

A microbial agent and oil refinery wastewater technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of easy use, improved treatment efficiency, and convenient transportation

Inactive Publication Date: 2007-12-19
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcomings of preferential control of pollutants in my country's water such as soluble aliphatic hydrocarbons, benzene series, sulfur-containing compounds and phthalates in the effluent water after oil separation-air flotation-chemical treatment, the present invention The purpose is to provide a microbial agent for treating oil refinery wastewater, its preparation method and its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1 Determination of various physical and chemical indicators of wastewater, the COD value is determined by the potassium dichromate method, the oil content is determined by the gravimetric method, the volatile phenol content is determined by the distilled 4-aminoantipyrine spectrophotometric method, and the organic matter composition is determined by the GC- Determined by MS analysis method. The results of wastewater quality analysis are as follows:

[0039] category

[0040] 2 Quantify the solid medium that has been subjected to high-temperature sterilization (121°C, 30 minutes) for future use. The formula of the solid medium is 0.5% beef extract, 1% peptone, 0.5% NaCl, 100g water, and 1.7% agar.

[0041] 3 Prefabricated strains and inoculated the prefabricated 14357, Na4, EM, CB4, PNCB3, 10-4 into sterilized solid medium for activation.

[0042] 4 Quantify the liquid medium that has been subjected to high-temperature sterilization (121° C., 30 minutes) for f...

Embodiment 2

[0050] 1 Wastewater preliminary test and mixed bacterial liquid preparation are the same as embodiment 1,

[0051] 2 Add the quantitative mixed bacterial solution (including medium) to the adsorbent to make the bacterial agent. The amount added is: add 1L medium to 1Kg adsorbent, and then pack it for low temperature storage.

[0052]3. Usage method: add activated sludge into the SBR reactor according to the dosage of 10%, add the inorganic salt medium containing 1000mg / l glucose for sludge cultivation, adopt the method of intermittent water change, change the water twice a day, and cultivate After 7 days, the sedimentation rate of the sludge reaches 10% in 30 minutes, and then gradually increases the proportion of oil refinery wastewater in the influent, so that the microorganisms in the sludge can be domesticated under the new living conditions gradually adapted, until all the influent water is oil refinery wastewater. Then add bacteria agent and 1% KH of the total COD value ...

Embodiment 3

[0058] 1. Various physical and chemical index determination methods of waste water are the same as embodiment 1. The result is as follows:

[0059] category

[0060] category

[0061] 2 Quantify the solid medium that has been subjected to high-temperature sterilization (121°C, 30 minutes) for future use. The formula of the solid medium is 0.3% beef extract, 1% peptone, 0.5% NaCl, 100g water, and 1.7% agar.

[0062] 3 Prefabricated strains and inoculated the prefabricated 14357, EM, PNCB3, 10-4 into sterilized solid medium for activation.

[0063] 4 Quantify the liquid medium that has been subjected to high-temperature sterilization (121°C, 30 minutes) for future use. The liquid medium formula is 0.3% beef extract, 1% peptone, 0.5% NaCl, and 100 g of water.

[0064] 5. Inoculate the activated strains into liquid medium and shake (37°C, 160 rpm) for 3 days.

[0065] 6 Count the cultured bacterial liquid, and then combine according to the quantitative ratio ...

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Abstract

The invention discloses a microbiological bacterium for processing waste water in oil refining, its preparing process and use. The bacterium comprises Rhodococcus, Microbacterium spp, bacillus, Candida and Brachybacterium by the ratio of 0-10:0-10:0-10:0-10:0-10:0-10. The bacterium can be activated for treating waste water.

Description

technical field [0001] The invention belongs to the field of environmental protection, and relates to a microbial agent, its preparation method and its application, and specifically relates to a variety of refractory compounds such as alkanes, aromatic hydrocarbons, substituted benzenes, and phenols, which are degraded according to the principles of microbial ecology. The symbiosis, intergrowth, synergy and other interaction relationships among highly efficient degrading bacteria are constructed into a microbial bacterial agent for treating oil refinery wastewater, and a preparation method and a use method of the bacterial agent. Background technique [0002] Refining wastewater is high-concentration refractory organic wastewater with a large discharge volume, mainly containing alkanes, aromatic hydrocarbons, volatile phenols and ammonia nitrogen and other pollutants. Wastewater with high substance content will cause great pollution to the environment and needs to be treated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01C12R1/07C12R1/13C12N1/14C12R1/72C02F3/34
Inventor 李旭东朱永光廖银章杨俊仕
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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