Process of obtaining element
A technology of elemene and extraction method, which is applied in the field of obtaining elemene from goldenrod, can solve the problem of not extracting the anticancer active ingredient elemene and the like
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Embodiment 1
[0012] Embodiment 1, a kind of method obtaining elemene, carries out following steps successively:
[0013] 1), gather 3000 grams of fresh leaves of Solidago decurrens;
[0014] 2), using a tissue grinder to pulverize into 1mm raw materials;
[0015] 3), the above-mentioned raw materials are extracted by steam distillation, and the extraction time is 10 hours. The oil and water were separated; 12.6 g of crude elemene was obtained as an oil.
[0016] Gained elemene crude product detects and analyzes components and content thereof through gas chromatography-mass spectrometry, and learns that: the total elemene content is 30%, and the weight ratio of δ-elemene and β-elemene is 3:1.
[0017] The above 10 g of crude elemene was purified and separated by conventional silica gel column chromatography, eluted with petroleum ether, the eluate was collected, and the solvent was removed; 2.4 g of pure elemene was obtained, with a purity of 96.2%.
Embodiment 2
[0018] Embodiment 2, a kind of method obtaining elemene, carries out following steps successively:
[0019] 1), gather 3000 grams of fresh leaves of Solidago decurrens;
[0020] 2), manually chopped into 1mm raw materials;
[0021] 3), using an ultrasonic instrument with a frequency of 15KHz and a power of 200W, add the above-mentioned raw materials into water at a ratio of 1:10 for ultrasonic extraction, and the extraction time is 60 minutes to obtain a feed liquid; filter the above feed liquid, centrifuge, and separate oil and water; Obtain 12 grams of crude elemene as an oil.
[0022] Gained elemene crude product, detects and analyzes composition and content through gas chromatography-mass spectrometry, learns: total elemene content is 35%, and the weight ratio of δ-elemene and β-elemene is 2.5:1.
[0023] The above 10 g of crude elemene was purified and separated by conventional silica gel column chromatography, eluted with petroleum ether, the eluate was collected, and...
Embodiment 3
[0024] Embodiment 3: a kind of method obtaining elemene, carries out following steps successively:
[0025] 1), collect Solidago decurrens root, after drying, obtain 3000 grams of dry rhizome;
[0026] 2), using a tissue pulverizer to pulverize the dried root to a raw material of 0.8mm;
[0027] 3), the above-mentioned raw materials are added into petroleum ether in a ratio of 1:10 and extracted by organic solvent extraction; the extraction time is 24 hours, leaching at room temperature for 3 times, filtering, centrifuging, and concentrating under reduced pressure to obtain a paste, Add absolute ethanol to purify and dry. That is, 30 grams of crude elemene was obtained as an oil.
[0028] Gained elemene crude product, detects and analyzes composition and content through gas chromatography-mass spectrometry, learns: total elemene content is 10%, and the weight ratio of δ-elemene and β-elemene is 1:1.
[0029] The above 20 g of crude elemene was purified and separated by con...
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