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Extraction of 4-androstenedione from oil phase of fermenting liquid

A kind of technology of androstenedione, fermentation broth

Inactive Publication Date: 2009-02-04
SHANGHAI ACEBRIGHT PHARMA GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by this invention is to overcome the deficiencies in the existing technology of extracting 4-AD, and to design a kind of extraction method with high extraction yield and less environmental pollution

Method used

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  • Extraction of 4-androstenedione from oil phase of fermenting liquid

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Embodiment Construction

[0024] (1) Add 30kg of table salt into 500L of fermentation broth, stir for 30 minutes, statically separate the layers for 8 hours, and collect 120L of the oil phase. Extract 5 times in the extraction tank, each time with 1:1 (V / V) ethanol extraction, and combine the 5 extractions to about 610L, (about 110L of raffinate is to be recovered). Concentrate 610L extract under reduced pressure in a concentration tank, cool and crystallize, and centrifugally filter to obtain about 20 kg of crude product; dissolve 20 kg of crude product with 300 L of acetone, filter, concentrate under reduced pressure in a concentration tank, cool and crystallize, and centrifugally filter to obtain about 15 kg of crude product; Dissolve 15kg of crude product in 300L ethyl acetate, add 0.3kg of activated carbon, stir and decolorize for 1 hour, filter, concentrate under reduced pressure in a concentration tank, cool and crystallize, and centrifugally filter to obtain about 12kg of crude product; dissolve...

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Abstract

Extraction of 4-androstenedione from oil phase of fermentation liquor achieves higher product purity, repeat use, no environmental pollution and short process route.

Description

Technical field: [0001] The invention belongs to the technical field of phytochemistry. It specifically relates to a method for extracting 4-androstenedione 4-Androstadiendione (4-AD) from the oily phase (vegetable oil) of the fermentation broth. Background technique: [0002] In the case of oil phase (vegetable oil) as the medium, phytosterols can be converted into 4-AD after special bacterial fermentation and biotransformation. Since 4-AD has strong lipophilicity, it mainly exists in the fermentation broth in the oil phase medium. Invention content: [0003] The technical problem to be solved by the present invention is to overcome the deficiencies in the existing process for extracting 4-AD, and design an extraction method with high extraction yield and less environmental pollution. [0004] The invention provides a method for extracting 4-androstenedione (4-AD) from the oily phase (vegetable oil) of the fermented liquid, the method comprising the following steps: ...

Claims

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Application Information

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IPC IPC(8): C07J1/00
Inventor 郑雄敏荣绍丰
Owner SHANGHAI ACEBRIGHT PHARMA GRP
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