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Prepn. method of macroporous Ago-Gel medium

A technology of agarose gel and medium, which is applied in the field of preparation of agarose gel medium, which can solve the problems of reducing equipment utilization and air bubble occupation, and achieve the effects of improving mechanical strength, reducing impact, and eliminating invalid use volume of equipment

Inactive Publication Date: 2009-04-15
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, a large number of bubbles generated when the coagulation medium removes the calcium carbonate porogen occupies a certain volume of the equipment, which will also reduce the utilization rate of the equipment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] At 90°C, 2 grams of iron oxide particles with a particle size of 1 μm and a distribution of 0.75-1.5 μm were uniformly dispersed in 100 mL of 60 g / L agarose solution by rapid stirring. Then quickly pour the suspension into 500mL organic phase containing 30g / L Span 60, and control the stirring speed at 1200rpm at 90°C; after reacting for 10min, quickly cool down to 20°C, solidify and form microspheres, and obtain coated iron oxide particles agarose gel medium. The collected microspheres were washed repeatedly with acetone and distilled water successively to remove the residual organic phase. After mixing 100mL of washed microspheres with 100ml of 1mol / L sodium hydroxide solution, add 500mg of sodium borohydride. The above mixture was reacted in a water-bath shaker at 40° C. and 170 rpm for 30 minutes; then 2 mL of epichlorohydrin was added, and the reaction was continued for 2 hours under the above conditions. After the reaction was completed, the mixture was repeatedl...

Embodiment 2

[0017] Change the solid particles into 10 grams of copper oxide particles with a particle size of 1.2 μm, and finally remove the copper oxide with 0.1M dilute sulfuric acid, and the rest are the same as in Example 1.

Embodiment 3

[0019] Change the solid particles into 6 grams, particle size 1 μm, and distribute 0.75-1.5 μm aluminum oxide particles, and the rest are the same as in Example 1.

[0020] Iron oxide among the above-mentioned embodiment 1 also can change into ferrous oxide.

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PUM

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Abstract

A process for preparing the macroreticular agarose gel medium features that the superfine particles of metallic oxide are used as pore forming agent and the bubbles can not be generated when said pore forming agent is removed.

Description

technical field [0001] The invention belongs to the preparation technology of agarose gel medium, in particular to a preparation method of macroporous agarose gel medium. Background technique [0002] Chromatographic separation using macroporous media is currently the preferred method for rapid separation of biomacromolecules. With the development of biotechnology, the demand for macroporous media is also increasing. [0003] Chinese patent 03130027.8 discloses a method for preparing a high-capacity macroporous agarose gel medium. The method mainly includes the preparation of suspension, emulsification reaction, solidification and crosslinking process, which disperses inorganic solid particle calcium carbonate in agarose aqueous solution to make suspension; then add the suspension to salad oil, and add emulsification Span 80, after stirring and emulsifying reaction, cooling down rapidly to form microspheres, then adding epichlorohydrin, and cross-linking under alkaline cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/30B01D15/08B01J20/291
CPCH04B1/7105H04B2201/70702
Inventor 徐伟王芝祥
Owner SHANGHAI INST OF PHARMA IND CO LTD