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Prepn. method of macroporous Ago-Gel medium

A kind of technology of agarose gel and medium, which is applied in the field of preparation of agarose gel medium, can solve the problems of reducing equipment utilization rate and air bubble occupation, and achieve the effect of improving mechanical strength, reducing impact and improving equipment utilization rate

Inactive Publication Date: 2006-11-29
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, a large number of bubbles generated when the coagulation medium removes the calcium carbonate porogen occupies a certain volume of the equipment, which will also reduce the utilization rate of the equipment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] At 90°C, 2 grams of iron oxide particles with a particle size of 1 μm and a distribution of 0.75-1.5 μm were uniformly dispersed in 100 mL of 60 g / L agarose solution by rapid stirring. Then the suspension was quickly poured into 500mL organic phase containing 30g / L Span60, at 90°C, the stirring speed was controlled at 1200rpm; after the reaction for 10min, the temperature was quickly cooled to 20°C, solidified to form microspheres, and obtained coated iron oxide particles Agarose gel medium. The collected microspheres were washed with acetone and distilled water repeatedly to remove residual organic phase. After mixing 100 mL of the washed microspheres with 100 mL of 1 mol / L sodium hydroxide solution, add 500 mg of sodium borohydride. The above mixture was reacted in a water bath shaker at 40° C. and 170 rpm for 30 minutes; then 2 mL of epichlorohydrin was added, and the reaction was continued for 2 hours under the above conditions. After the reaction is completed, the dist...

Embodiment 2

[0017] Change the solid particles to 10 grams of copper oxide particles with a particle size of 1.2 μm, and finally remove the copper oxide with 0.1M dilute sulfuric acid, and the rest is the same as in Example 1.

Embodiment 3

[0019] The solid particles are changed to 6 grams, with a particle size of 1 μm and a distribution of 0.75 to 1.5 μm alumina particles, and the rest are the same as in Example 1.

[0020] The iron oxide in the above Example 1 can also be replaced with ferrous oxide.

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PUM

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Abstract

A process for preparing the macroreticular agarose gel medium features that the superfine particles of metallic oxide are used as pore forming agent and the bubbles can not be generated when said pore forming agent is removed.

Description

Technical field [0001] The invention belongs to the preparation technology of agarose gel medium, and particularly relates to a preparation method of macroporous agarose gel medium. Background technique [0002] The application of macroporous media for chromatographic separation is currently the preferred method for rapid separation of biological macromolecules. With the development of biotechnology, the demand for macroporous media is also increasing. [0003] Chinese patent 03130027.8 discloses a method for preparing a high-capacity macroporous agarose gel medium. The method mainly includes suspension preparation, emulsification reaction, solidification and cross-linking process. It disperses inorganic solid particulate calcium carbonate in agarose aqueous solution to form a suspension; then adds the suspension to salad oil and adds the emulsification Reagent Span 80, after stirring the emulsification reaction, quickly cool down to form microspheres, then add epichlorohydrin, a...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30B01D15/08B01J20/291
CPCH04B1/7105H04B2201/70702
Inventor 徐伟王芝祥
Owner SHANGHAI INST OF PHARMA IND