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Magnetic separating column and its use in separating biological samples

A technology for magnetic separation and biological samples, applied in magnetic separation, solid separation, magnetism of inorganic materials, etc., can solve the problems of low magnetic induction intensity and no improvement in separation efficiency, and achieve uniform size, easy for large-scale production, and easy to transport and carry Effect

Inactive Publication Date: 2009-10-07
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low magnetic induction intensity generated by the ordinary permanent magnet magnetic field, it is difficult to achieve fast and efficient separation of target isolates
At present, there is no device that utilizes the internal magnetic field in biological samples to enhance the separation effect and improve the separation efficiency

Method used

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  • Magnetic separating column and its use in separating biological samples
  • Magnetic separating column and its use in separating biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of filler pellets: prepared by splash quenching method.

[0042] Take three parts of soft iron material, its composition and weight percentage are respectively: iron 99.0% + carbon 0.04%, iron 99.5% + carbon 0.001%, and iron 99.8% + carbon 0.01%, and the balance contains a very small amount of aluminum, Sodium, zinc, chromium, nickel, copper, manganese and other elements. The three materials are melted at a high temperature of 1500°C-1800°C, and the molten soft iron is sprayed at a speed of 3-50 m / s, dropped from a high altitude or centrifugally thrown out, and the surface tension of the liquid phase is used to automatically shrink into a ball. Falling into the water, it suddenly condenses into a small iron ball.

Embodiment 2

[0044] Particle size screening: Most of the particle sizes of the three kinds of material pellets prepared in Example 1 are 0.2-1mm, and the magnetic properties of the iron balls are different with different particle sizes. The present invention adopts a sampling sieve to carry out particle size screening: a sampling sieve with a suitable mesh number is selected to screen pellets with target particle sizes of 0.01-0.1mm, 0.1-0.2mm and 0.2-1mm respectively. Iron balls with a larger particle size of 0.2-1 mm can be used to process a large number of samples, and are suitable for separating magnetically labeled biological samples with strong magnetic responses. Iron balls with a smaller particle size of 0.01-0.1 mm or 0.1-0.2 mm can generate a magnetic field with a larger gradient, which is suitable for separating magnetically labeled biological samples with weak magnetic response.

[0045] Density Screening: The main component of filler pellets is iron. During the preparation of...

Embodiment 3

[0048] Improving magnetic permeability: The stress generated by quenching makes the magnetic properties of the balls unable to meet the requirements of sorting materials. It is necessary to eliminate the stress through a suitable annealing process, so as to improve the magnetic permeability of the small iron ball and reduce its coercive force. Raise the temperature to 400-900°C with the furnace, control the temperature to 700-1000°C, keep it for 1-5 hours, and cool it down to 400-900°C at a controlled speed, preferably raise the temperature to 800°C with the furnace, control the temperature to 900°C, and keep it for 4 hours. Cool down to 500°C at controlled speed. In order to avoid the oxidation of the balls, the annealing of the balls is carried out in a vacuum furnace or a hydrogen atmosphere. The annealed pellets have fine grains, uniform structure, low hardness, low coercive force (Hc=30~150A / m) and high maximum magnetic permeability (μ max =4000~9000), which meets the b...

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Abstract

The invention relates to a magnetic separation column and its application in the separation of biological samples. The column (2) is filled with magnetic soft iron balls with a particle size of 0.01-0.1mm, 0.1-0.2mm and 0.2-1mm. During the sorting process, the sample is added from the upper flow inlet (5) and flows through it under the action of gravity. The packing column (2), outlet (3), and the push plug (4) are used to apply pressure to elute the retained sample. The magnetic separation column of the present invention can generate a high-gradient magnetic field under the action of an external magnetic field, so that biological samples such as various cells, subcellular substances, bacteria, viruses, nucleic acids or proteins can be separated simply, effectively, conveniently and quickly.

Description

technical field [0001] The invention belongs to the field of biological separation devices, and more particularly relates to a device for separating biological samples by using magnetism. Background technique [0002] With the rapid development of modern biotechnology, magnetic separation technology has been widely used in the separation of biological samples. The principle is: the surface modification of magnetic beads (particle size <5 μm) can recognize and bind cells and subcellular substances Biomolecules of biological samples such as bacteria, viruses, nucleic acids or proteins form magnetic bead-biomolecular complexes. The complex is used to capture the target biological sample, and then retained under the action of an external magnetic field to achieve separation. Due to the low magnetic induction intensity generated by the ordinary permanent magnet magnetic field, it is difficult to achieve fast and efficient separation of target isolates. At present, there is n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B03C1/02H01F1/14B22F9/06B05D7/24C21D11/00C21D1/26
Inventor 沈鹤柏周海清兰天张诗渊
Owner SHANGHAI NORMAL UNIVERSITY
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