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Shell and tube orientation arrangement structure cell holder and its preparation method

An orientation arrangement and cell scaffold technology, which is applied in the field of biodegradable cell scaffolds for tissue engineering, and can solve the problems of inability to make cell scaffolds with tubular orientation structures, etc.

Inactive Publication Date: 2010-02-24
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies cannot be used to make a cell scaffold with a tube-like orientation structure, especially one that has both tubular primary holes and tiny holes (secondary holes) on the tube wall to allow the tubes to communicate. Tubular Oriented Cell Scaffold

Method used

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  • Shell and tube orientation arrangement structure cell holder and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: get molecular weight and be 0.5 gram poly-L-lactide (PLLA) of 40000 and be made into 1% (weight) solution with dioxane, be placed in the plastic straight cylinder of aforementioned device. Cool the solution from 28°C to the metal rod at the lower end of the device at a cooling rate of 0.2°C / min until the crystal growth is complete until 0°C. Then freeze-dry for 48 hours to completely remove the solvent, and obtain the cell scaffold with the primary pore diameter of 280±20 microns in the aligned structure of the tubes.

Embodiment 2

[0028] Example 2: 1.5 grams of poly DL-lactide (PDLLA) with a molecular weight of 60000 was prepared into a 3% (by weight) solution with tetrahydrofuran, and placed in the plastic straight cylinder of the aforementioned device. The solution was cooled from 26°C to the metal rod at the lower end of the device at a cooling rate of 0.4°C / min until -20°C to complete the crystal growth. Then freeze-dry for 48 hours to remove the solvent completely, and obtain the cell scaffold with the primary pore size of 150±20 microns in the tubular orientation structure.

Embodiment 3

[0029] Embodiment 3: get 2.5 grams of copoly(L-lactide / DL-lactide) (PLLA-co-PDLLA) that molecular weight is 50000 and be made into 5% (weight) solution with acetone, place the aforementioned device In a straight plastic barrel. The solution was cooled from 29°C to the metal rod at the lower end of the device at a cooling rate of 0.8°C / min until -80°C to complete the crystal growth. Then freeze-dry for 48 hours to completely remove the solvent, and obtain the cell scaffold with the primary pore diameter of 100±10 microns in the aligned structure of the tubes.

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Abstract

The present invention relates to a shell-and-tube orientation arranged structure cell scaffold and its preparation method. The material of said cell scaffold includes synthetic aliphatic polyester bio-degradable high-molecular material or natural bio-degradable high-molecular material or their mixture, in the material the growth factor bio-degradable active substance or bio-degradable inorganic substance is contained and used as third component. The solvent for preparing shell-and-tube orientation arranged structure cell scaffold is first solvent or first solvent in which the second solvent ismixed. Said invention adopts a progressively cooling technique from bottom to top, cooling speed is 0.01-2.5deg.C / min and cooling range is t37 to-196deg.C, finally the shell-and tube orientation structure cell scaffold for tissue engineering can be obtained. Said invention also provides the concrete structure of said cell scaffold and the concrete steps of its preparation method.

Description

technical field [0001] The invention relates to a biodegradable cell scaffold for tissue engineering with a tube-and-tube orientation arrangement structure. [0002] The present invention also relates to the preparation method of the above-mentioned cell scaffold. Background technique [0003] Tissue engineering is a science that combines the principles of engineering with the principles of cytology and tissue biology to replace defective (aged, damaged or diseased) tissues and organs. It is to culture and expand autologous cells or stem cells in vitro on scaffolds prepared from biological materials and having the same configuration as the tissue or organ to be repaired, and then implanted into the lesion in the body to allow them to multiply. Ultimately, the purpose of repairing and reconstructing tissues or organs and restoring functions is achieved. Tissues and organs reconstructed through tissue engineering can not only be mass-produced like artificial substitutes, but...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/00C12N11/02C12M3/00A61F2/02C12R1/91C12N5/00
Inventor 王身国杨飞贝建中
Owner INST OF CHEM CHINESE ACAD OF SCI