Microbial fertilizer of chelates of composite amino acid for spray irrigation, drop irrigation, and production method
A compound amino acid and microbial fertilizer technology, applied in organic fertilizers, fertilizer mixtures, applications, etc., can solve problems such as chemical clogging that has not attracted enough attention, biological clogging, etc., and achieve the effects of inhibiting the growth of harmful microorganisms, low cost, and high fertilizer efficiency
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Embodiment 1
[0041] Embodiment one: the preparation of the microbial fertilizer of spray drip irrigation compound amino acid chelate
[0042] Add 400ml of water to the reaction kettle, add Ba(OH) 2 400ml, add NaOH 20ml, add donkey bone, donkey skin, cottonseed cake, hoof horns in total 800-1000g, hydrolyze at 100℃-110℃, keep for 6-8h, cool the hydrolyzed solution to 60℃, add a small amount For elemental compounds, heat to 95-110°C and keep for 0.4-1.0h. After the chelate reaction solution is cooled to room temperature, add 0.01% by weight of beneficial microbial flora and mix, and its beneficial microbial flora is composed of four kinds of bacteria: nitrogen-fixing bacteria, phosphorus bacteria, potassium bacteria, and bacillus. The microbial fertilizer product of liquid compound amino acid chelate, see the attached figure 1 .
Embodiment 2
[0043] Embodiment two: the preparation of amino acid trace element chelate
[0044] In order to increase the use effect of this new product, the present invention designs the scheme of adding amino acid trace element chelate. The main components of amino acid trace element chelate are: boron, manganese, zinc, molybdenum and other trace elements.
[0045] In order to make the amount of nutrients added reasonable, that is, effective without being too high, and at the same time to achieve a reasonable ratio of various ingredients without any physical and chemical changes, we have carried out the formulation of the amino acid trace element chelate. optimization.
[0046] The present invention adopts the optimization method of combining single factor test and orthogonal test, and investigates the application effects of different proportions and different content levels of each main component through pot experiments of three crops, selects the best proportion, and finally determine...
Embodiment 3
[0048] Embodiment three: Beneficial microbial strain and its function
[0049] 1. Microbial strain XHS0026N, identified by microbiology as Klebsiella pneumoniae subsp pneumoniae (klebsiella pneumoniae subsp pneumoniaiae) CGMCC.No.1642.
[0050] Strain function: The content of main auxin substances in the XHS0026N culture solution was significantly higher than that of the original strain, and the indole acetic acid substances increased most significantly. In the shake flask test, the content levels in different repetitions ranged from 50-200ppm, and the average content reached more than 66ppm, which was about 15% higher than the starting strain. In addition, the content of gibberellin and cytokinin also increased to varying degrees. The bacterium can also produce a large amount of exopolysaccharides during the fermentation process, and has significant nitrogen-fixing properties.
[0051] 2. The XHS0012K strain in the microbial fertilizer was determined to be Bacillus mucilagi...
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