Process of windowing breeding egg and hatching chicken embryo

A technology for breeding eggs and eggshells, applied in biochemical equipment and methods, embryonic cells, microorganisms, etc., can solve the problems of 0 hatching rate, poor operability, complicated and cumbersome production methods, etc., and achieve high hatching rate and operability. Strong performance, simple and convenient technology

Inactive Publication Date: 2007-08-22
湖南省畜牧兽医研究所
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AI Technical Summary

Problems solved by technology

The number of samples in each group is less than 40, and the hatching rate is 33.3% to 76.5%, but there is no control group data in the experiment
In addition, the author reported in 2005 that using the same method to experiment with unhatched eggs, the hatching rate turned out to be 0, which is obviously inconsistent with domestic and foreign research reports
[0012] Generally speaking, the above research methods still have the following defects at present: the production method is complicated and cumbersome, and cannot be produced in batches, and the survival rate of breeding eggs after the experimental operation is low (generally lower than 30%), and some of them reach more than 60%, but the sample content is extremely high. less, poor operability

Method used

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  • Process of windowing breeding egg and hatching chicken embryo
  • Process of windowing breeding egg and hatching chicken embryo

Examples

Experimental program
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Embodiment Construction

[0029] Take the block as a triangle as an example:

[0030] In the aseptic room, use an egg opener to cut a triangle with a side length of about 5mm on the equator of the egg. Only the outer shell is cut, and the inner shell membrane is not cut. The lower edge of the wide seam is glued about 1mm 2 The double-sided adhesive tape is used to cut the inner shell membrane of the cut piece and then turn the cut piece and stick it.

[0031] Transfer the eggs into a sterile room, use a 10# scalpel, cut open the inner shell membrane on both sides of the narrower slit, pick up the cut piece with a blade, turn it around 180 degrees and fix it on the eggshell with double-sided tape, and use a glass The injection needle injects the target substance through the window to manipulate the embryo.

[0032] Cut the 1.5cm wide medical paper tape larger than the triangular cut piece, stick it directly on the cut piece as the center, smooth it with a little force, and then apply a layer of 502 gl...

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Abstract

The present invention discloses process of windowing hatching egg and hatching chicken embryo, and the process includes windowing hatching egg, embryo operation, sealing and hatching. The present invention features the windowing hatching egg including sterilizing hatching egg, cutting one or several polygonal or circular slots without opening the inner membrane, cutting the inner membrane in bacteria-free condition, and turning the cut inner membrane for convenient embryo operation. Compared with available technology, the present invention has the advantages of simple operation and capacity of reaching hatching rate over 50 %.

Description

technical field [0001] The invention relates to a technique for window-opening operation of breeding eggs and hatching chicken embryos, in particular to a technique for window-opening operation and hatching chicken embryos by utilizing eggshells of breeding eggs. Background technique [0002] At present, the technology of opening eggs to operate chicken embryos is mainly used in the following four aspects: 1) poultry transgenic technology using blastocyst cells as a carrier; 2) poultry transgenic technology using primordial germ cells as a carrier; 3) using pluripotent cells to preserve Poultry germplasm resource technology; 4) Use chicken embryos as animal embryo development research models to study gene (or drug) functions. [0003] How to operate a soybean-sized embryo in an egg, the method should be simple and convenient, and at the same time, it must ensure a high hatching rate, which has always been a difficult problem. At present, according to the mode of incubation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/073
Inventor 燕海峰朱立军肖兵南
Owner 湖南省畜牧兽医研究所
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