Sesquiterplactone in dandelion and its use of resisting Gram's positive bacteria

A technology of sesquiterpene lactone and dandelion, applied in the field of medicine, can solve problems such as unclear chemical composition

Inactive Publication Date: 2007-08-29
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are few domestic research reports on the chemical constituents of Dandelion plants, and the chemical constituents of the medicinal dandelion Taraxacum officinale are mostly cited. At present, after searching, it is found that Ling Yun’s research group has conducted a research report on the chemical constituents of Mongolian Dandelion [Ling Yun, Bao Yanyan, Zhu Lili, Zheng Junhua, Cai Shaoqing, Xiao Yue, Research on Chemical Constituents of Dandelion, Chinese Journal of Pharmaceutical Sciences, 1997, 32, 584-586; Ling Yun, Bao Yanyan, Zhang Yonglin, Cai Shaoqing, Zheng Junhua, Research on Chemical Constituents of Dandelion, Journal of Naval General Hospital, 1998, 11, 167- 169], a total of 5 compounds were obtained: β-sitosterol, quercetin, caffeic acid, chlorogenic acid and luteolin-7-O-glucoside, while other chemical components are not yet clear

Method used

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  • Sesquiterplactone in dandelion and its use of resisting Gram's positive bacteria
  • Sesquiterplactone in dandelion and its use of resisting Gram's positive bacteria
  • Sesquiterplactone in dandelion and its use of resisting Gram's positive bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 The separation of mongolian dandelion lactone A:

[0020] 1.1 Instruments and reagents

[0021] The melting point was measured with a microscopic melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on a Polax-2L automatic polarimeter produced in Japan; the infrared spectrum IR was measured by a Bruker Vector-22 infrared spectrometer, and pressed by KBr; Spectra were measured with a Shimadzu UV-240 ultraviolet spectrophotometer; hydrogen nuclear magnetic resonance 1 H-NMR, carbon nuclear magnetic resonance spectrum 13C-NMR and 2D NMR were determined by INOVA superconducting nuclear magnetic resonance spectrometer (VARIAN INOVA-400 MHz) (tetramethylsilyl ether TMS was used as internal standard); electrospray mass spectrometry ESI-MS was determined by Bruker Esquire 3000+ mass spectrometer, Silica gel for column chromatography (100-200, 200-300 and 300-400 mesh) and silic...

Embodiment 2

[0034] Example 2: Detection of Pseudo-Guaiacane Sesquiterpene Lactone Compound's Ability to Inhibit Gram-positive Bacteria

[0035] 2.1 Principle:

[0036] The "tube-and-disk method" is used to study the in vitro antibacterial activity of the test drug, which is to use the test drug added in the Oxford cup to diffuse into the medium inoculated with the test bacteria, thereby inhibiting the growth of the bacteria. The diameter of the bacterial circle was used to determine the antibacterial activity of the test drug.

[0037] 2.2 Detection of bacteria:

[0038] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0039] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0040] 2.3 Test drugs:

[0041] DMSO: DMSO solvent control;

[0042] Norfloxacin: 1.25mg / ml;

[0043] Mongolian dandelion lactone A (compound 1): 5.0 mg...

Embodiment 3

[0060] Embodiment 3: the comparison of Compound 1 in five kinds of Dandelion plant rhizomes and aerial part stems and leaves

[0061] Dandelion (Taraxacum mongolicum Hand-Mazz), Dandelion Alkaline (Taraxacum sinicum Kitag), Dandelion Jehol (Taraxacum platypecidum Diels), Dandelion Northeast (Taraxacum ohwianum Kitag), Dandelion inverse (Taraxacum grypodon Dahlst) and Xing'an dandelion (Taraxacum falcilobum Kitag) each 20 grams were collected and identified by Professor Peng Hua from Kunming Institute of Botany, Chinese Academy of Sciences.

[0062] Waters high performance liquid chromatography (Waters HPLC, 2695 separation system includes quaternary pump, column thermostat autosampler; 2996 ultraviolet diode matrix detector, Empower chromatographic operating software and WatersSymmetry  RP-18 4.6mm×250mm, 5μm chromatographic column) was used to detect the content of compound 1.

[0063] Treatment of test samples: Weigh 0.5 g (±0.1 mg) of each sample after drying in the shade...

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Abstract

The invention relates to a faked guaiane sesquiterpene lactone compound-Morgan blowball lactone A and the medicinable salt. The chemical name is 1-ketone-11(R)-hydroxyl-3, 5, 1(10)-trienin-faked lignum benedictum-8 alpha, 12-lactone. The compound could make medicine vehicle or medicine medicinal preparation with medicinable salt. The medicine contains other antibacterial druy. The preparation includes tablet, granule, capsule, oral liquid, drop pills, injection, skin penetrating agent, aerosol, etc. the compound could be used in medicine to prevent and cure anti gram positive fungus.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular to a pseudoguaiacol sesquiterpene lactone isolated from dandelion and a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing these compounds and their use in anti-infective drugs. use. technical background [0002] Staphylococcus aureus, a Gram-positive coccus, and methylcillin-resistant Staphylococcus aureus (MRSA), a strain that began to emerge in the 1980s, are a major source of microbial epidemics in hospitals and clinics The main infections caused by human are boils, pustules, toxic epidermal decomposition, pneumonia, osteomyelitis, acute myocarditis, mastitis, cystitis, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, pharyngitis, Cervicitis, pelvic inflammatory disease, and abscesses of the muscles, skin, urogenital tract, and central nervous system. Therefore, it is a pathogenic bacteria that has a great impact on human he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93A61K31/365A61P31/04A61K36/288
Inventor 赵昱杨柳青施树云王晓雨徐艳孙莲莉陶巧凤曾苏
Owner ZHEJIANG UNIV
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