Method for separating polysaccharide against growth of cancer cells from dictyophord

Abstract

Separation of polysaccharide in red bamboo fungus teleblemata is carried out by drying, crushing, extracting alcohol with water, depositing, separating out educt, drying, preparing dried educt into solution, deproteinizing in Sevag method to alcohol precipitate, separating out educt while drying to prepare into 12mg / mL solution, separating in chromatography column to obtain DRVP1. It has excellent inhibiting function for tumor-cell growth.

Description

technical field The invention relates to a method for extracting polysaccharides from edible fungi, in particular to a method for separating polysaccharides contained in the fungus receptacle of Dictyophora edulis. Background technique Malignant tumors are major refractory diseases that seriously endanger human health, and drug therapy is still an important means of tumor prevention and treatment. my country is rich in medicinal plant resources, and there are broad prospects for developing new anti-tumor drugs with independent intellectual property rights using natural drug resources. Dictyophora rubrovalvata is a basidiomycete belonging to Eumycota, Basidiomycotina, Gasteromycetes, Phallales, Phallaceae, Dictyophora (Dictyophora), the fruiting body of Dictyophora edulis is 20-33cm high; the buds are oval, dark red, 4-5cm×4-6cm in size, the rhizomes are white, and will turn blue-purple after injury; The receptacle is dark red, the cap is bell-shaped or blunt conical, 5-6...

AI Technical Summary

Problems solved by technology

However, during the processing of finished products of Dictyophora edulis, producers often use its fruiting bodies, and remove the caps and receptacles as leftovers (the weight ratio of fruiting bodies to caps and receptacles is 1:1.3), resulting in huge Waste, studies have shown that dried mushrooms are rich in polysaccharides, amino acids, proteins, vitamins and beneficial metal elements

And so far, there is no report on the polysaccharides that can inhibit the growth of tumor cells on the Dictyophora edulis fungus.

Images