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Use of comprehensive two-dimensional gas chromatography in flavored beer development

A technology of two-dimensional gas chromatography and analysis technology, which is applied in the field of flavor beer development, can solve problems such as the analysis and detection of beer flavor substances without comprehensive two-dimensional gas chromatography technology, and achieve the effect of expanding the application field

Inactive Publication Date: 2007-10-24
粟学俐
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  • Summary
  • Abstract
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Problems solved by technology

At present, no comprehensive two-dimensional gas chromatography technology has been used in the analysis and detection of beer flavor substances at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0005] (1) Experimental equipment and experimental conditions

[0006] 1. Instrument comprehensive two-dimensional gas chromatography system is composed of HP6890 gas chromatograph (Agilent Technologies, Wilmington, DE, USA) with hydrogen flame detector (FID) and KT-2001 cold spray modulator (Zoex Corp., Lincoln, NE, USA). A Pegasus III mass spectrometer (Leco Corporation, St. Joseph, MI, USA) employs a 70 eV electron bombardment ionization source.

[0007] 2. Experimental conditions

[0008] GC×GC column system adopts DB-Petro (100% dimethylpolysiloxane) and DB-17ht (50% phenylmethylpolysiloxane) double column system. Inlet temperature: 250°C; column front pressure: 600kpa, constant pressure; carrier gas: helium, purity 99.9995%; split ratio: 1:30; injection volume: 5μl; modulation period: 5s; ℃ (1min), rising to 220℃ (30min) at 3℃ / min; FID acquisition frequency: 100Hz; TOFMS acquisition frequency and acquisition range: 50Hz, 35-500u; ion source temperature: 220℃; transfer...

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PUM

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Abstract

The invention relates to a two-dimension gas spectrum analysis technique, used in flavor bear development, characterized in that the gas component of flavor bear extracted by solid extract method, is treated by two-dimension gas spectrum analysis and mass spectrum analysis couple technique to check the chemical components of flavor bear. The invention has the advantages that the invention can obtain hundreds of flavor components to build a bear flavor mass fingerprint spectrum database, to complete the quality check of quality auxiliary control in general production and new-product development. The built bear flavor mass fingerprint spectrum database can be used to develop new flavor bear product with high quality. And the invention expands the application of two-dimension gas spectrum.

Description

technical field [0001] The invention relates to the field of flavor beer development. Background technique [0002] Beer has a wide variety of flavor substances with low content, containing more than 2,000 ingredients such as alcohols, aldehydes, esters, ketones, acids, sulfides, aromatic substances and other organic substances and inorganic ions. It contains about 92% water, about 3-4% alcohol, and about 3% flavor ingredients. The content and level of various flavor substances play a decisive role in the aroma and quality of beer. Therefore, the composition of beer products must be identified. Using appropriate methods to determine beer flavor components and constructing a beer flavor substance fingerprint database is a key technical means for the world's major beer groups to carry out high-level quality control. Regarding the analysis technology of beer flavor components, according to the latest literature search, static headspace (SHS) was fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 粟学俐许国旺赵三红郑孝平朱书奎张与红鲁荣元熊航行
Owner 粟学俐
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