Support-colouring means
A carrier and color technology, used in dyeing methods, chemical instruments and methods, organic compounds/hydrides/coordination complex catalysts, etc., can solve the problems of environmental pollution and time-consuming chemical inks, and achieve fast carrier coloring, Overcome the blockage problem, the effect of good resolution
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[0058] Example 1 : Use of hemoglobin
[0059] 1) Photoreversible inhibition of hemoglobin
[0060] In the dark, at 20 °C for 1 h, the solution in aqueous solution (32 μM ) in bovine hemoglobin. The final concentration of DMSO should not exceed 5%. NPE-diazo concentrations varied between 50-100 equivalents relative to hemoglobin. The coupling reaction was stopped by adding 100 mM acetate buffer solution, pH 4.4 (1 equivalent to NPE-diazo). The contact time is 10-30 minutes. The reaction mixture is then dialyzed against water at a temperature of 4-20° C. for a period of 1-16 hours and against 1000 volumes of water. Conjugates were stored at 20°C.
[0061] Percent oxidation inhibition of ABTS [2,2'-azinobis(3-ethylbenzothiazolinone-6-sulfonic acid diammonium salt), 1 mM] in the presence of 1 mM hydrogen peroxide before dialysis Assays were performed on 96-well microtiter plates (Costar, Corning Incorporated) in sodium acetate buffer (50 mM, pH 5). The number of bound m...
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