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Autologous fat stem cell constructing tissue engineering cartilage

A kind of fat stem cell, cartilage technology, applied in the field of medicine and biomedical engineering, can solve the problem of unsuccessful

Inactive Publication Date: 2007-11-28
SHANGHAI TISSUE ENG LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The application of adult stem cells has greatly expanded the source of seed cells, but at present only autologous adult stem cells are used for the repair of cartilage tissue defects in small animals, and it has not been successful in using them in large mammals.

Method used

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  • Autologous fat stem cell constructing tissue engineering cartilage
  • Autologous fat stem cell constructing tissue engineering cartilage
  • Autologous fat stem cell constructing tissue engineering cartilage

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0076] The preparation method of the tissue engineered cartilage of the present invention is simple and convenient, and a certain amount of seed cells are mixed with pharmaceutically acceptable biodegradable materials.

[0077] The shape of the tissue-engineered cartilage graft of the present invention is not particularly limited. Typically, the shape of the graft is the same or substantially the same shape as the mammalian cartilage defect. Take the graft similar to the shape of articular cartilage as an example, its volume is 0.02-6cm 3 .

[0078] When the biodegradable material of the present invention is an injectable material, adjust the concentration of chondrocytes with the liquid material; when the biodegradable material of the present invention is a solid material, adjust the concentration of the seed cell suspension with the culture medium, and then use the The degradable material is mixed. When mixing, the ratio of the culture medium to the degradable material is ...

Embodiment 1

[0091] Isolation and culture of adipose-derived stem cells

[0092] Obtain pig neck and back fat tissue under sterile conditions, shred it fully, transfer it into a centrifuge tube, add an equal volume of 0.075% type I collagenase for digestion, 37°C, 40min, add serum-containing culture medium to stop digestion, centrifuge at 1390r / min, 10min, discard the supernatant, filter with a 150-mesh cell strainer, and then centrifuge at 1390r / min for 5min, discard the supernatant, add low-sugar DMEM culture medium containing 10% fetal bovine serum to mix the cells, and divide 5×10 4 / cm 2 Inoculated at 100mm 2 cultured in a petri dish. After 24 hours, it can be seen that the spindle cells adhere to the wall. When the cells grow to nearly 80%-90% confluent at the bottom of the culture dish, they are digested with 0.25% trypsin + 0.02% EDTA, passaged, and continue to be cultured. The second generation of cells is collected for use Inoculation material.

Embodiment 2

[0094] Preparation of bioscaffold composite polylactic acid / polyglycolic acid (polylactic acid / polyglicolic acid, PLA / PGA, PLGA)

[0095] Method: Take about 40mg of non-woven PGA fiber and evenly embed it into a prefabricated cylindrical (9mm in diameter, 5mm in height) silica gel mold, add about 0.3ml of 1% PLA dichloroethane solution, and take it out and store it in vacuum after it dries naturally. . See Figure 3.

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Abstract

The present invention discloses one kind of cartilage graft, which includes pharmaceutically acceptable biodegradable material and fat stem cell and is in the shape matching the cartilage defect part to be repaired. The tissue engineering cartilage constructed with the autologous fat stem cell may be used in treating cartilage impairment effectively. The present invention also provides the preparation process and use of the tissue engineering cartilage.

Description

technical field [0001] The invention relates to the fields of medicine and biomedical engineering, and more specifically relates to tissue engineered cartilage and its preparation method and application. Background technique [0002] Defects or damage to cartilage can be caused by congenital malformations, trauma, infection, tumors, or diseases such as osteochondritis. Due to the low self-repair ability of cartilage tissue after injury, the treatment of cartilage defect or injury has always been a great challenge in reconstructive surgery. At present, the commonly used clinical treatment methods include: free or pedicled autologous cartilage transplantation (mainly taken from costal cartilage), free transplantation of allogeneic cartilage, autologous or allogeneic chondrocyte injection transplantation, periosteum or perichondrium transplantation, and artificial prosthesis, etc. . However, these methods have obvious disadvantages, such as: few sources of donors for autologo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/50
Inventor 崔磊曹谊林尹烁
Owner SHANGHAI TISSUE ENG LIFE SCI
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