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Method for improving the main efficacy composition of panax notoginseng through zymolysis

A technology of Panax notoginseng and efficacy, applied in the field of bioengineering, can solve problems such as toxicity and underutilization, and achieve the effects of simple operation process, easy biotransformation, and easy culture.

Inactive Publication Date: 2008-01-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main functional components of Panax notoginseng have not been fully utilized. In addition, there are potentially toxic or difficult-to-decompose components in Panax notoginseng, which cause great problems for the extraction of the main functional components of Panax notoginseng or the utilization of the human body. Therefore, it is necessary to use microbial methods Improve and increase the extraction rate of the main components of Panax notoginseng, modify the main active components, and increase their bioavailability
At present, there are no reports on the microbial transformation and bioavailability of Panax notoginseng at home and abroad

Method used

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  • Method for improving the main efficacy composition of panax notoginseng through zymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Transformation effect test of different strains on Panax notoginseng leaves

[0026] Refer to the flow chart of Figure 1, using Bacillus licheniformis, Bacillus subtilis and Bacillus natto as the strains to transform the leaves of Panax notoginseng respectively, and the total saponins content of the transformed Panax notoginseng leaves are determined and extracted. The conditions and The results are as follows:

[0027] Each strain was stored on a slant in the LB medium, activated at 37°C for 24 hours, and then inoculated on the liquid seed medium. The liquid seed culture medium is LB, no agar, pH 7, and the liquid seed culture is 25-30 ml in a 250 ml shake bottle, the fermentation temperature is 37°C, the shaker speed is 180-200r / min, and the culture is obtained for 12-24 hours. liquid.

[0028] Panax notoginseng leaves were crushed by adding 2ml of distilled water per gram of Panax notoginseng and sterilized at 115°C for 15 minutes to obtain Panax notoginseng tr...

Embodiment 2

[0033] Example 2 Transformation effect test of different strains on Panax notoginseng roots

[0034] According to the method of Example 1, the root of Panax notoginseng was used as the transformation object, and the total saponins content of the transformed Panax notoginseng leaves were measured and extracted. The results are shown in Table 2.

[0035] Table 2 Effects of transformation of different strains on the content and extraction rate of total saponins of Panax notoginseng roots

[0036]

[0037] Bacillus subtilis

[0038] The control group in Table 2 is the untransformed Panax notoginseng roots. It can be seen that the total saponins content of Panax notoginseng roots can be significantly increased through biological fermentation transformation. Among them, the transformation ability of Bacillus licheniformis is the most significant, and the total saponins content reaches 5.9 mg / ml. . Compared with the control group, the total saponins content of the thr...

Embodiment 3

[0039] Example 3 Transformation effect test of Panax notoginseng leaves with different transformation times

[0040] According to the method of Example 1, only Bacillus subtilis was used as the strain, and the leaves of Panax notoginseng were used for transformation. The total saponin content of Panax notoginseng leaves after different times of transformation was measured and extracted. The results are shown in Table 3.

[0041] Table 3 Effects of different transformation times on the content and extraction rate of total saponins in Panax notoginseng leaves

[0042]

[0043] The comparative analysis in Table 3 found that different conversion times can significantly affect the content of total saponins of Panax notoginseng roots. Among them, the 72-hour conversion has the most significant impact on the total saponins content and the extraction rate of total saponins, while the change after 72 hours is not very large. .

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Abstract

The invention discloses a method for improving notoginseng main functional components through fermentation, which comprises following steps: (1)culturing bacillus LB inclined surface seed for 24 hours, moving it to LB liquid culture medium, culturing and getting culture liquid; (2)mixing notoginseng with water, disinfecting and getting notoginseng convertible culture medium; (3)inoculating culture liquid got in step (1)to notoginseng convertible culture medium, converting notoginseng; (4)drying converted notoginseng until the weight is constant, getting target product. The main functional component content after biological fermentation is increased by 4-6 times, the extract liquid amount is increased by about two times. The invention provides good method and platform for further development and utilization of Chinese traditional medicine.

Description

Technical field [0001] The invention relates to the field of bioengineering, in particular to a method for improving the main functional components (total saponins) of Panax notoginseng medicinal materials by using a microbial method. technical background [0002] my country has a long history of traditional Chinese medicine and abundant resources of traditional Chinese medicine. In order to ensure the health of the people, the international community is becoming more and more aware of the important role of traditional Chinese medicine in the treatment and prevention of diseases. However, in recent decades, my country has made slow progress in the modernization of traditional Chinese medicine, mainly due to the outdated processing technology of traditional Chinese medicine and the unclear effective ingredients in traditional Chinese medicine. In recent years, the emerging development of traditional Chinese medicine fermentation pharmaceutical technology is a new research field th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258C12P1/00C12R1/07A61K125/00A61K127/00A61K135/00
Inventor 陈启和何国庆项云阮晖章海锋
Owner ZHEJIANG UNIV
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