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Flat-plate bacterial colony photographic reprinting apparatus and method

A technology of photocopying device and flat plate, which is applied in the field of microbiology, can solve the problems of high pressure on edge colonies, different photocopying effects, increased bacterial contamination, etc., and achieve the effect of reducing operation steps, good photocopying effect, and easy promotion and use

Inactive Publication Date: 2008-01-02
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the bacterial density is slightly higher, it is easy to cause contamination between colonies
[0011] 3) Since the edge part is easier to be stressed than the central part, the edge part is easier to photocopy than the central part; for the same reason, in order to take care of the photocopying effect of the colony in the middle of the plate, the colony on the edge is often over-pressed and becomes flat Lawn
[0012] 4) The photocopying effect is different for faster-growing, larger-sized colonies and slower-growing, smaller-sized colonies
[0013] 5) It is necessary to take out flannelette or other photocopying media and fix them on the cylindrical surface every time, which not only increases the operation steps but also increases the possibility of bacterial contamination
These measures have high requirements for operation, not only the operation is too troublesome but also high requirements for materials

Method used

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  • Flat-plate bacterial colony photographic reprinting apparatus and method
  • Flat-plate bacterial colony photographic reprinting apparatus and method
  • Flat-plate bacterial colony photographic reprinting apparatus and method

Examples

Experimental program
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Embodiment 1

[0050] Embodiment one: the making of plate colony photocopying device of the present invention

[0051] Materials: commercially available common toothpicks, which can be toothpicks with pointed ends, or toothpicks with a pointed end and a flat end; rubber bands or strings (for example, about 40 cm); a set of Petri dishes.

[0052] Production process: Use a string or rubber band to bundle the toothpicks into a bundle with a diameter slightly smaller than the bottom cover of the petri dish, and gently hit it on a flat table to make the bottom and top surfaces flat. The inside can be turned easily without being too tight. So far the device is finished, put it in an oven and dry it, then it can be sterilized and used.

[0053] In this embodiment, the rubber bands used can be replaced by other elastic bands, such as spring wire. The string used can be replaced with a ribbon such as silk thread.

[0054] As shown in Figure 4, Figure 5a and Figure 5b, the plate colony photocopying...

Embodiment 2

[0056] Embodiment two: the further making of plate colony photocopying device of the present invention

[0057] Materials: a set of Petri dishes; a bottle of ordinary glue; the device made in Example 1.

[0058] Production process: the other end (that is, the end not in contact with the plate bacterium colony) opposite to the top of the densely packed tip of the device made in embodiment one is coated with common glue, wait for a while and wait for the glue to penetrate; The above-mentioned support body) is buckled on the glued side, and the petri dish and toothpick are gently turned over. So far the device is finished, put it in an oven and dry it, then it can be sterilized and used.

[0059] As shown in Figures 6 and 7, the plate colony photocopying device of the present invention also includes a support body 5; in the device 3, the other end, namely the bottom 6, which is opposite to the top 4 with densely packed tips is fixed on the support body 5 . Therefore, the devic...

Embodiment 3

[0060] Embodiment three: the further improvement of the plate colony photocopying device of the present invention

[0061] Materials: A set of Petri dishes. The device made in embodiment two.

[0062] Manufacturing process: fasten the upper cover on the densely tipped top of the device made in Example 1 (that is, the flat surface that will be in contact with bacteria). So far the tool is finished, put it into the oven and dry it, then it can be sterilized and used.

[0063] As shown in FIG. 8 and FIG. 9 , the plate colony photocopying device of the present invention further includes a detachable top cover 7 for covering the top 4 of the device 3 with densely packed tips. Therefore, contamination of the tip can be avoided, and the device can be easily sterilized and reused.

[0064] Of course, a top cover can also be directly added to the device made in Embodiment 1.

[0065] It should be noted that the top cover can be expanded into a sleeve (not shown in the figure) that ...

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Abstract

The invention relates to a method for culturing and screening the bacterial in the microorganism field in particular to a device and a method of flat bacterial colony photolithograph. The flat bacterial colony photolithograph device comprises the following parts: a plurality of rod-like objects with not less than a tip, a ribbon object for tying up a plurality of rod-like objects, a cylindrical device which is tied up a plurality of rod-like objects by the ribbon object, wherein the tip of every rod-like object is the same direction and forms the top which gathers the tip, the diameter of the cylindrical device is less than the diameter of culture plate bottom. The invention also provides a method of the flat bacterial colony photolithograph. The device doesn't need the high-requirement material, which has the simple technology without any intermediate material, and has the simple using method and the clear effect.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a method for culturing and screening bacteria, in particular to a device and a method for plate colony photocopying. Background technique [0002] In the process of in situ hybridization, auxotrophic and drug-resistant bacterial screening, it is necessary to transfer the colonies on one plate to another plate containing the corresponding antibiotic or treatment factor, thereby forming a replica plate of the mother plate, that is, a daughter plate. According to the growth of the strains on the daughter board, find the strains that meet the requirements, and then find the target strains on the mother board. [0003] The origin and principle of photocopying plate method [0004] In 1952, J. Lederberg and his wife proposed the replica plating method for the first time in their paper "Plate Copy Culture Method and Indirect Selection of Bacterial Mutants", and proved that the generation of bacte...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/00C12Q1/18
CPCC12M33/02C12M23/10
Inventor 徐民俊周世宁田小群
Owner SUN YAT SEN UNIV
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