Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root
A technique of asexual reproduction and nuclear sterility, applied in the fields of botany equipment and methods, application, plant genetic improvement, etc., can solve the problem of not being able to completely preserve parents or common strains, and achieve commercial promotion, low cost, and reduced workload effect
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Embodiment 1
[0023] The present invention utilizes ratoons to carry out asexual reproduction method of cotton nucleus sterile dual-purpose line, and its steps are:
[0024] A. In seed production areas where cotton plants can survive the winter, that is, areas where the lowest winter temperature is greater than 10°C, plant cotton nucleus sterile lines, identify fertile and sterile plants at the bud stage, and remove 4 / 5~3 / 5, mark the remaining 1 / 5~2 / 5 of the fertile plants; use insect pollination or artificial pollination during the flowering and boll setting period, and harvest the seeds on the sterile plants after the boll is set for the preparation of cotton Hybrid use;
[0025] B. After the seeds are harvested, all the cotton plants stay in the cotton field to survive the winter naturally and become ratoons;
[0026] C. Prune the cotton plants remaining in the cotton field with fruit tree shears in the next year, first pruning the branches, and then trim the cotton piles with 3-5 regenerat...
Embodiment 2
[0029] The present invention utilizes ratoons to carry out asexual reproduction method of cotton nucleus sterile restorer line or ordinary cotton line, and its steps are:
[0030] A. In seed production areas where cotton plants can survive the winter, that is, areas where the lowest temperature in winter is greater than 10°C, plant cotton nucleus sterile restorer lines or ordinary cotton lines, and use insect pollination or artificial pollination during the flowering and bolling period, and harvest cotton after boll setting Seed on the plant;
[0031] B. After the seeds are harvested, all the cotton plants stay in the cotton field to survive the winter naturally and become ratoons;
[0032] C. Prune the cotton plants left in the cotton field with fruit tree shears in the next year. When the number of regenerated seedlings from the pruned cotton piles reaches 3-5 roots, trim them and leave 1 root for each pile. Regenerative seedlings, during the flowering and boll setting period, u...
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