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Tissue culture propagating method for heavy metal super-enriched thlaspi caerulescens

A technology of celestial alba and a super-accumulating plant, which is applied in the field of tissue culture and reproduction of the heavy metal hyper-accumulation plant celestial stamens, can solve the problems of small biomass, production and application limitations, slow growth of celestial stamens, and the like, Achieve the effect of reducing production costs, saving seedlings, and stabilizing genetic traits

Inactive Publication Date: 2008-01-30
CHINA UNIV OF MINING & TECH (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the slow growth and small biomass of C. indigo have caused limitations in production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the tissue culture propagation of cerulean

[0034] 1. Cut off the stem tip of the adult celery plant, rinse it with running water, disinfect the surface with 70% alcohol for 30 seconds, disinfect with 0.1% mercuric dichloride for 8-10 minutes, rinse with sterile water 3-5 times, and dry it with filter paper surface moisture;

[0035] 2. Cut the sterilized materials into 1 cm leafy shoot tips, inoculate them into the start-up medium, place them in the cultivation room, light them for 16 hours per day, cultivate them for 21 days, and clustered sprouts will appear;

[0036] The components and proportions contained in the starting medium are as follows: each liter of starting basic medium contains 100 mg of inositol, 250 mg of hydrolyzed milk protein, vitamin B 2 1 mg, 1 mg of 6-benzyl adenine, 0.2 mg of naphthaleneacetic acid, pH=5.8; the starting basic medium is 4736.43 mg / L MS medium;

[0037] 3. Trim the clustered sprouts in test tubes that are not less...

Embodiment 2

[0045] Embodiment 2: Tissue culture and propagation of celestia nigra

[0046] 1. Cut off the stem tip of the adult celery plant, rinse it with running water, disinfect the surface with 70% alcohol for 30 seconds, disinfect with 0.1% mercuric dichloride for 8-10 minutes, rinse with sterile water 3-5 times, and dry it with filter paper surface moisture;

[0047] 2. Cut the sterilized materials into 1 cm leafy stem tips and inoculate them into the start-up medium, place them in the cultivation room, 16 hours of light per day, cultivate for 21 days, and clustered sprouts appear;

[0048] The components and proportions contained in the starting medium are as follows: each liter of starting basic medium contains 100 mg of inositol, 250 mg of hydrolyzed milk protein, vitamin B 2 2 mg, 1 mg of 6-benzyl adenine, 0.2 mg of naphthaleneacetic acid, pH=5.8; the starting basic medium is 4736.43 mg / L MS medium;

[0049] 3. Trim the clustered sprouts in test tubes with no less than 2.5 cm...

Embodiment 3

[0056] Embodiment 3: Tissue culture propagation of cerulean

[0057] 1. Inoculate the stem section of the seedlings with buds of the clipped C. indigo indica seeds into the starting medium, place them in the cultivation room, light for 16 hours / day, and cultivate them for 21 days, and clustered buds appear;

[0058] The components and proportions contained in the starting medium are as follows: each liter of starting basic medium contains 100 mg of inositol, 250 mg of hydrolyzed milk protein, vitamin B 2 2 mg, 1 mg of 6-benzyl adenine, 0. mg of naphthaleneacetic acid, pH=5.8; the starting basic medium is 4736.43 mg / L MS medium;

[0059] 2. Trim the clustered sprouts in test tubes that are more than 2.5 cm into stems with 2-3 nodes, inoculate them in the proliferation medium, place them in the culture room, and cultivate them for 21 days with 16 hours of light per day. A large number of dense clusters will appear. bud;

[0060] The components and proportions contained in the ...

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Abstract

The invention relates to tissue culture and reproduction method of heavy metal super enriched plant of thlaspi caerulescens and comprises the following steps: disinfected stem tip / stem node of the thlaspi caerulescens with the length of 0.8-1.2cm and leaves or disinfected seedling thereof with bud and stem is inoculated to starting culture medium to be cultivated into multiple bud seedling; next the multiple bud seedling is inoculated to enrichment medium to be cultivated into dense multiply bud; then the obtained dense multiply bud is placed into basic culture medium for seedling strengthening; thereafter the multiply bud is transferred to rooting culture medium for rooting; then the multiply bud is transferred to culture medium of Grant solution for water cultivation for seedling strengthening; finally the strengthened seedling is transferred to garden matrix for planting and the tissue of thlaspi caerulescens thereby is obtained. The method not only can exclude affects of external conditions, and can be carried out all the year around, save culture land and reduce production cost, but also can obtain a plurality of strong seedling in short term and be applied in large-scaled production.

Description

technical field [0001] The invention belongs to the technical field of artificial propagation and cultivation methods of plants; in particular, it relates to a method for tissue culture and propagation of a heavy metal hyperaccumulation plant, Cerulean indigo. Background technique [0002] Thlaspi caerulescens is an annual or perennial herbaceous plant native to central, western and southern Europe. It is a typical heavy metal hyperaccumulator plant, in which the accumulated zinc content is as high as 25000-30000 mg / kg, did not show any injury symptoms or growth inhibition, and most plants showed poisoning symptoms when the accumulated zinc reached 100 mg / kg; at the same time, the accumulated cadmium content was as high as 1800 mg / kg, which was far beyond what other ordinary plants could tolerate limit. Since the 1980s, the idea of ​​using phytoremediation of heavy metal-contaminated soil and water has attracted increasing attention from academic and industrial circles at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 张玉秀徐进
Owner CHINA UNIV OF MINING & TECH (BEIJING)
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