Tissue culture propagating method for heavy metal super-enriched thlaspi caerulescens

Abstract

The invention relates to tissue culture and reproduction method of heavy metal super enriched plant of thlaspi caerulescens and comprises the following steps: disinfected stem tip / stem node of the thlaspi caerulescens with the length of 0.8-1.2cm and leaves or disinfected seedling thereof with bud and stem is inoculated to starting culture medium to be cultivated into multiple bud seedling; next the multiple bud seedling is inoculated to enrichment medium to be cultivated into dense multiply bud; then the obtained dense multiply bud is placed into basic culture medium for seedling strengthening; thereafter the multiply bud is transferred to rooting culture medium for rooting; then the multiply bud is transferred to culture medium of Grant solution for water cultivation for seedling strengthening; finally the strengthened seedling is transferred to garden matrix for planting and the tissue of thlaspi caerulescens thereby is obtained. The method not only can exclude affects of external conditions, and can be carried out all the year around, save culture land and reduce production cost, but also can obtain a plurality of strong seedling in short term and be applied in large-scaled production.

Description

technical field

[0001] The invention belongs to the technical field of artificial propagation and cultivation methods of plants; in particular, it relates to a method for tissue culture and propagation of a heavy metal hyperaccumulation plant, Cerulean indigo. Background technique

[0002] Thlaspi caerulescens is an annual or perennial herbaceous plant native to central, western and southern Europe. It is a typical heavy metal hyperaccumulator plant, in which the accumulated zinc content is as high as 25000-30000 mg / kg, did not show any injury symptoms or growth inhibition, and most plants showed poisoning symptoms when the accumulated zinc reached 100 mg / kg; at the same time, the accumulated cadmium content was as high as 1800 mg / kg, which was far beyond what other ordinary plants could tolerate limit. Since the 1980s, the idea of ​​using phytoremediation of heavy metal-contaminated soil and water has attracted increasing attention from academic and industrial circles at ...