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Low dosage livestock semen and production method and application thereof

A low-dose, livestock-based technology, applied in the direction of drug combinations, diseases, and medical raw materials derived from mammals, can solve the problems of not being able to meet the needs of production and research, and achieve the goals of reducing breeding costs, improving utilization rates, and expanding production Effect

Active Publication Date: 2008-01-30
李喜和 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of genetic resources of excellent or specific male mammals, it still cannot meet the needs of production and research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The recipe for bovine semen:

[0033] The target livestock-bovine sperm concentration is 1 million bovine sperm / 0.25ml,

[0034] Exotic livestock-sheep semen concentration is 20 million bovine sperm / 0.25ml

[0035] Tris-A (frozen balance solution A) 50%

[0036] Tris-B (Cryobalance B) 50% with 12% Glycerin Cryoprotectant

[0037] The production process is:

[0038] 1. Making low-dose bovine frozen semen

[0039] ①Take the semen of fresh milk beef cattle (origin: Inner Mongolia Autonomous Region, China, breed: Bos tarurs) containing 1 ml of sperm with a concentration of 1 billion, and dilute it to 16 million sperm / ml with Tris-A (frozen balance solution A) 62.5ml, and then placed in a 4°C refrigerator for more than 90 minutes;

[0040] ②Take 25 ml of fresh sheep semen (origin: Inner Mongolia Autonomous Region, China, breed: Ovis aries) with a concentration of 1 billion sperm / ml, centrifuge at 2000 rpm for 5 minutes, and dilute it with Tris-A to 320 million sperm / ml....

Embodiment 2

[0046] The recipe for goat semen:

[0047] Target livestock-goat sperm concentration is 2 million bovine sperm / 0.25ml;

[0048] Exotic livestock-sheep semen concentration is 20 million bovine sperm / 0.25ml;

[0049] Tris-A (frozen balance solution A) 50%

[0050] Tris-B (Cryobalance B) 50% with 12% Glycerin Cryoprotectant

[0051] The production process is:

[0052] 1. Making low-dose goat frozen semen

[0053] ① Take the semen of fresh goat (place of origin: Inner Mongolia Autonomous Region, China, species: Caprahircas) containing 1 ml of sperm with a concentration of 1 billion, and dilute it with Tris-A (frozen balance solution A) to 31.3 ml of goat semen of 32 million sperm / ml, Then put it in a refrigerator at 4°C for more than 90 minutes;

[0054] ②Take 15 ml of fresh sheep semen (origin: Inner Mongolia Autonomous Region, China, breed: Ovisaries) with a concentration of 1 billion sperm / ml, centrifuge at 2000 rpm for 5 minutes, and dilute it with Tris-A to 320 million s...

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PUM

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Abstract

The present invention relates to a novel propagation technology which is based on the principle of heterogeneous livestock semen assistant function, so as to produce the low-dose target livestock semen and use the product to implement the in vivo / in vitro fertilization. The main contents include producing the low-dose target livestock fresh and frozen semen by adding heterogeneous livestock semen and using the target livestock fresh and frozen semen produced by adding heterogeneous livestock semen to carry out the in vitro fertilization and artificial fertilization. The invention can use a small amount of semen of the target livestock (1 to 50 percent of the normally required concentration) to reach the normal fertilization and pregnant level through the assistant function of the heterogeneous livestock semen. The technology can greatly improve the utilization rate of the target livestock, which is used for breeding and propagation of the target livestock, as well as the infertility caused by poor semen quality indicators of rare semen and poor vitality etc. of special target male livestock. In addition, the technology can also be taken as a novel method of the study of reproductive biology.

Description

technical field [0001] The invention relates to a low-dose livestock semen and its production method and application. Background technique [0002] Livestock-based frozen semen, artificial insemination and in vitro fertilization techniques are widely used in production breeding and reproductive biology research. Mammalian fertilization usually requires sperm to reach a certain number before it can be achieved. Traditional theory holds that only when the required number of spermatozoa is reached can fertilization be achieved by sperm that are sufficiently vigorous. However, due to the limitation of genetic resources of excellent or specific male mammals, it still cannot meet the needs of production and research. Contents of the invention [0003] The object of the present invention is to provide a low-dose livestock semen and its production method and application, which can overcome the above-mentioned defects. The present invention uses the semen of different kinds of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/52A61P15/00
Inventor 李喜和周文忠张国富钱松晋王建国
Owner 李喜和
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