Material for detecting micro-trace dimethyl methyl phosphomate and method for making same

Abstract

The invention relates to a material used for trace dimethyl methyl phosphate detection and the preparation method which belong to the sensing material for organophosphorus pesticide detection and the preparation method. The material of the invention consists of TPPS and flour. The preparation of the material comprises the processes: firstly, plant esterase is produced with flour on the market as the raw material and TPPS solution is prepared; then the plant esterase and the TPPS solution are sequentially fixed on a K9 glass sheet activated with glutaric dialdehyde; after the K9 glass sheet is dried with air, the finished product is achieved. As the material of the invention has simple components, low cost; the preparation method of the material is simple and the operation of the preparation is simple and convenient; the product prepared according to the invention has the advantages of convenience use, good detecting sensitivity and low detection limit that the product can detect effectively micro-trace DMMP; the invention can be widely used for the detection of trace organophosphorus pesticide residues in vegetables, fruits and other agricultural products, for food and environmental safety assessment, and for the monitor of the organophosphorus pesticide pollution of food and environment.

Description

technical field [0001] The invention belongs to a sensing material for detecting organophosphorus pesticides and a preparation method thereof, in particular to a material for detecting trace amounts of dimethyl methyl phosphate and a preparation method thereof. Background technique [0002] Dimethyl methyl phosphate (DMMP) is an organophosphorus pesticide. Since the 1980s, organophosphorus pesticides have become the most widely used pesticides in agricultural and forestry production and postharvest preservation. The long-term use of organophosphorus pesticides in large quantities has produced extensive side effects while gaining benefits. The residues of organophosphorus pesticides in vegetables, fruits and other foods that people eat daily can easily cause teratogenic, carcinogenic and mutagenic accumulation in the human body through the action of the food chain, and cause serious pollution and harm to the ecological environment. At present, in terms of food safety and eve...

AI Technical Summary

Problems solved by technology

However, since they all only immobilize the enzyme, in addition to the sample to be tested, additional substrates and chromogenic reagents need to be added during detection.

This not only increases the detection steps, prolongs the detection time, but is inconvenient to use

Moreover, due to the sensitivity of the chromogenic agent itself and the limitation of the detection method, its detection limit is high, and it cannot effectively detect the pesticide residues with low content, and this slight pesticide residue may cause chronic diseases and still threaten people's health. life safety

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