Tigridiopalma magnifica C. Chen tissue culturing and propagating method

A tissue culture and tiger face flower technology, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve problems such as failure to achieve reproductive effect, slow reproduction speed, and small seeds, and achieves simple and affordable methods. Good results with good seedling quality

Inactive Publication Date: 2010-12-15
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Huyanhua mainly relies on ramets to reproduce under natural conditions, and the speed of reproduction is slow; while Huyanhua has few seeds, small seeds, difficult to germinate, and can not reach the ideal reproductive effect when cultivated artificially.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] During the growing season, select vigorous young leaves of Huyan flower, rinse them under tap water, soak them in 70% alcohol for 10 seconds, then sterilize them with 0.1% mercury liter solution for 5 minutes, rinse them with sterile water 4 times, and cut into 1cm Inoculate a square of about 1cm into the culture medium (each liter contains 5.0mg of 6-benzylpurine, 0.5mg of naphthaleneacetic acid, 30g of sucrose and 7g of agar, the rest is MS, pH5.5), at a temperature of 28°C and an illumination of 1500lx , light 12h / day, cultured for 10 days and then transferred to the same fresh medium, continued to cultivate for 50 days to form adventitious buds on the explants. The induced adventitious buds were cut and then subcultured in the same medium as above, at a temperature of 28°C, an illumination of 1500lx, and a light of 12h / day, for the proliferation of adventitious buds, and 40 days was a subculture cycle. The multiplication factor after each subculture multiplication c...

Embodiment 2

[0021] During the growing season, select vigorous young leaves of Huyan flower, wash them under tap water, soak them in 75% alcohol for 10 seconds, then sterilize them with 0.05% mercury liter solution for 10 minutes, rinse them with sterile water 5 times, and cut into 1cm ×1cm square, inoculated into the culture medium (each liter contains 10.0mg of 6-benzylpurine, 1.0mg of naphthaleneacetic acid, 20g of sucrose and 5g of agar, the rest is MS, pH5.8), the culture temperature is 30°C, the light intensity is 2000lx, Light for 8h / day, then transfer to the same fresh medium after 5 days, and continue culturing for 45 days to form adventitious buds on the explants. The induced adventitious buds were cut and then subcultured in the same medium as above to proliferate the adventitious buds. 30 days was a subculture cycle, and the multiplication factor after each subculture cycle was 5 times. capable of multiple generations. When the buds are about 2 to 3 cm high, they are cut and i...

Embodiment 3

[0023] During the growing season, select vigorous young leaves of Huyan flower, wash them under tap water, soak them in 70% alcohol for 30s, then disinfect them with 1.5% sodium hypochlorite solution for 10 minutes, rinse them with sterile water 4 times, and cut into 1cm ×1cm square, inoculated into the culture medium (each liter contains 7.0mg of 6-benzylpurine, 0.7mg of naphthaleneacetic acid, 30g of sucrose and 7g of agar, the rest is MS, pH5.6, culture temperature 26°C, illuminance 1800lx, light 10h / day, after 5 days, transfer to the same fresh medium again, and continue to cultivate for 50 days to form adventitious buds on the explants. Subculture after cutting the induced adventitious buds is carried out on the same medium as above The proliferation of adventitious buds, 35 days is a subculture multiplication cycle, and the multiplication factor after every 1 subculture multiplication cycle is 4 times.Can carry out multiple subcultures.When the bud height is about 2~3cm, ...

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Abstract

The present invention relates to a tissue culture and propagating method of Tigridiopalma magnifica C. Chen; the young leaves of eugonic Tigridiopalma magnifica C. Chen are selected to be the explants in the growing season and after being sterilized, the explants are inoculated into an MS culture medium with 5.0 - 10.0 mg of 6-benzyl purine and 0.5- 1.0 mg of naphthyl acetic acid every liter until the indefinite but is sprouted; after being induced and cut, the indefinite bud is cultivated in the same culture medium for propagation in the pattern of successive transfer culture; when the bud height is about 2 - 3 cm, the bud is cut to inoculated into the 1 / 2MS radication culture medium with 0.5 - 1.0 mg of naphthyl acetic acid and 0.5 - 1 mg of active carbon every liter until the bud is normally redicated; at last, when the tube seedling grows to 3 - 4 cm, the seedling is trained in the natural light and then transplanted. The method of the present invention is simple with a small amount of investment, fast germination, high stability and robust seedlings; the seedlings have high quality and the resistance is excellent; the radication rat of the Tigridiopalma magnifica C. Chen can reach 85 to 95 percent and the survival rate can reach 85 to 95 percent.

Description

technical field [0001] The invention relates to a method for breeding and cultivating the tiger-face flower, in particular to a method for propagating the tiger-face flower by tissue culture technology. Background technique [0002] Tiger face flower (Tigridiopalma magnifica C.Chen), also known as big lotus, bear's paw, etc., is the only single-species plant of the genus Tigridiopalma magnifica. It is mainly distributed in the Ehuangzhang Nature Reserve in Yangchun, Guangdong Province. It is very narrow, and due to the destruction of the ecological environment, artificial over-excavation and low natural fertility, the number is extremely rare. It is a rare and endangered plant unique to Guangdong Province in my country, and has been included in the list of rare and endangered plants in China. [0003] Huyanhua has huge leaves with a diameter of more than 50 cm. It has beautiful leaf shape, strong shade tolerance, small and exquisite flower buds, and high ornamental value. It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 曾宋君李龙娜吴坤林陈之林段俊郑枫
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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