Tigridiopalma magnifica C. Chen tissue culturing and propagating method
A technology of tissue culture and tiger face flower, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of ineffective reproduction, slow reproduction speed, small seeds, etc., and achieve simple and affordable methods, high growth Good, good quality seedlings
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[0018] Example 1:
[0019] In the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under tap water, soak them in 70% alcohol for 10 seconds, then disinfect with 0.1% mercury solution for 5 minutes, rinse them with sterile water 4 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 5.0mg 6-benzylpurine, 0.5mg naphthaleneacetic acid, 30g sucrose and 7g agar, the rest is MS, pH 5.5), at a temperature of 28°C, an illumination of 1500lx, Light for 12h / day, culture for 10 days and then transfer to the same fresh medium as before, continue to cultivate for 50 days to form adventitious buds on the explants. After cutting the induced adventitious buds, they were subcultured in the same medium as the above. The adventitious buds were proliferated at a temperature of 28°C, an illuminance of 1500lx, and an illumination of 12h / day. 40 days constitutes a secondary proliferation cycle. The proliferation fold after eac...
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[0020] Example 2:
[0021] During the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under running water, soak them in 75% alcohol for 10 seconds, then disinfect them with 0.05% mercury solution for 10 minutes, rinse them with sterile water 5 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 10.0mg 6-benzylpurine, 1.0mg naphthaleneacetic acid, 20g sucrose and 5g agar, the rest is MS, pH 5.8), culture temperature 30℃, illumination 2000lx, light 8h / day, after 5 days, transfer to the same fresh medium as before, and continue to cultivate for 45 days to form adventitious buds on the explants. After cutting the induced adventitious buds, they are subcultured in the same medium as the above for adventitious bud proliferation. 30 days is a sub-generation cycle, and the multiplication multiple after each sub-generation cycle is 5 times. Can carry out multiple generations. When the shoot height is about 2~3c...
Example Embodiment
[0022] Example 3:
[0023] In the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under running water, soak them in 70% alcohol for 30s, then disinfect with 1.5% sodium hypochlorite solution for 10 minutes, rinse with sterile water 4 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 7.0mg 6-benzylpurine, 0.7mg naphthaleneacetic acid, 30g sucrose and 7g agar, the rest is MS, pH 5.6, culture temperature 26℃, illumination 1800lx, illumination 10h / Day, after 5 days, transfer to the same fresh medium as before, and continue to cultivate for 50 days to form adventitious buds on the explants. The induced adventitious buds are cut and then subcultured in the same medium as the above For bud proliferation, 35 days is a subgeneration cycle, and the multiplication multiple after each subgeneration cycle is 4 times. It can be subcultured multiple times. When the bud height is about 2 to 3 cm, cut and inoculate ...
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