Tigridiopalma magnifica C. Chen tissue culturing and propagating method

A technology of tissue culture and tiger face flower, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of ineffective reproduction, slow reproduction speed, small seeds, etc., and achieve simple and affordable methods, high growth Good, good quality seedlings

Inactive Publication Date: 2008-03-12
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Huyanhua mainly relies on ramets to reproduce under natural conditions, and the speed of reproduction is slow; while Huyanhua

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1:

[0019] In the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under tap water, soak them in 70% alcohol for 10 seconds, then disinfect with 0.1% mercury solution for 5 minutes, rinse them with sterile water 4 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 5.0mg 6-benzylpurine, 0.5mg naphthaleneacetic acid, 30g sucrose and 7g agar, the rest is MS, pH 5.5), at a temperature of 28°C, an illumination of 1500lx, Light for 12h / day, culture for 10 days and then transfer to the same fresh medium as before, continue to cultivate for 50 days to form adventitious buds on the explants. After cutting the induced adventitious buds, they were subcultured in the same medium as the above. The adventitious buds were proliferated at a temperature of 28°C, an illuminance of 1500lx, and an illumination of 12h / day. 40 days constitutes a secondary proliferation cycle. The proliferation fold after eac...

Example Embodiment

[0020] Example 2:

[0021] During the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under running water, soak them in 75% alcohol for 10 seconds, then disinfect them with 0.05% mercury solution for 10 minutes, rinse them with sterile water 5 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 10.0mg 6-benzylpurine, 1.0mg naphthaleneacetic acid, 20g sucrose and 5g agar, the rest is MS, pH 5.8), culture temperature 30℃, illumination 2000lx, light 8h / day, after 5 days, transfer to the same fresh medium as before, and continue to cultivate for 45 days to form adventitious buds on the explants. After cutting the induced adventitious buds, they are subcultured in the same medium as the above for adventitious bud proliferation. 30 days is a sub-generation cycle, and the multiplication multiple after each sub-generation cycle is 5 times. Can carry out multiple generations. When the shoot height is about 2~3c...

Example Embodiment

[0022] Example 3:

[0023] In the growing season, select the vigorously growing young leaves of Huyan flowers, rinse them under running water, soak them in 70% alcohol for 30s, then disinfect with 1.5% sodium hypochlorite solution for 10 minutes, rinse with sterile water 4 times, and cut them into 1cm ×1cm square, inoculate the culture medium (each liter contains 7.0mg 6-benzylpurine, 0.7mg naphthaleneacetic acid, 30g sucrose and 7g agar, the rest is MS, pH 5.6, culture temperature 26℃, illumination 1800lx, illumination 10h / Day, after 5 days, transfer to the same fresh medium as before, and continue to cultivate for 50 days to form adventitious buds on the explants. The induced adventitious buds are cut and then subcultured in the same medium as the above For bud proliferation, 35 days is a subgeneration cycle, and the multiplication multiple after each subgeneration cycle is 4 times. It can be subcultured multiple times. When the bud height is about 2 to 3 cm, cut and inoculate ...

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Abstract

The present invention relates to a tissue culture and propagating method of Tigridiopalma magnifica C. Chen; the young leaves of eugonic Tigridiopalma magnifica C. Chen are selected to be the explants in the growing season and after being sterilized, the explants are inoculated into an MS culture medium with 5.0 - 10.0 mg of 6-benzyl purine and 0.5- 1.0 mg of naphthyl acetic acid every liter until the indefinite but is sprouted; after being induced and cut, the indefinite bud is cultivated in the same culture medium for propagation in the pattern of successive transfer culture; when the bud height is about 2 - 3 cm, the bud is cut to inoculated into the 1/2MS radication culture medium with 0.5 - 1.0 mg of naphthyl acetic acid and 0.5 - 1 mg of active carbon every liter until the bud is normally redicated; at last, when the tube seedling grows to 3 - 4 cm, the seedling is trained in the natural light and then transplanted. The method of the present invention is simple with a small amount of investment, fast germination, high stability and robust seedlings; the seedlings have high quality and the resistance is excellent; the radication rat of the Tigridiopalma magnifica C. Chen can reach 85 to 95 percent and the survival rate can reach 85 to 95 percent.

Description

technical field [0001] The invention relates to a method for breeding and cultivating the tiger-face flower, in particular to a method for propagating the tiger-face flower by tissue culture technology. Background technique [0002] Tiger face flower (Tigridiopalma magnifica C.Chen), also known as big lotus, bear's paw, etc., is the only single-species plant of the genus Tigridiopalma magnifica. It is mainly distributed in the Ehuangzhang Nature Reserve in Yangchun, Guangdong Province. It is very narrow, and due to the destruction of the ecological environment, artificial over-excavation and low natural fertility, the number is extremely rare. It is a rare and endangered plant unique to Guangdong Province in my country, and has been included in the list of rare and endangered plants in China. [0003] Huyanhua has huge leaves with a diameter of more than 50 cm. It has beautiful leaf shape, strong shade tolerance, small and exquisite flower buds, and high ornamental value. It ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 曾宋君李龙娜吴坤林陈之林段俊郑枫
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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