The rooting method of the strange walnut Ulak tissue culture seedling in the bottle

A technology of the exotic walnut and urak group, which is applied in the field of plant cultivation, can solve the problems of chaotic source of lines, difficult rooting, and low rooting rate of branch cuttings, and achieves the effect of accelerating the promotion speed.

Active Publication Date: 2017-01-11
上海菁艺生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the exotic walnuts in the domestic market are all scions, the source of the strains is chaotic, and there is no clone rooted seedlings.
The rooting rate of exotic walnut (vlach) branch cuttings is low, and plant tissue culture, as a vegetative propagation method, has become an inevitable way to propagate this excellent clone, but due to the difficulty of rooting, it has not yet formed an industrialization
At present, there are many kinds of walnut rootstocks in the nursery stock market, good and bad are mixed, and most of them are obtained by sowing seeds. There is a problem of character separation in offspring, and it is difficult to stably inherit good characters. Take root, unable to form factory production

Method used

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  • The rooting method of the strange walnut Ulak tissue culture seedling in the bottle
  • The rooting method of the strange walnut Ulak tissue culture seedling in the bottle
  • The rooting method of the strange walnut Ulak tissue culture seedling in the bottle

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 A method of rooting in a bottle of exotic walnut Ulak tissue culture seedlings

[0025] A kind of rooting method in the strange walnut Ulak tissue culture seedling bottle, it comprises the following steps:

[0026] (1) Select the exotic walnut tissue culture seedlings (from the laboratory of Professor Xi Shengke, Chinese Academy of Forestry) with a plant height of 3 cm, a subculture cycle of 20 days, and green or dark green leaf color as rooting materials, and remove the basal leaves and axillary buds Leave a 1 cm stem segment to the base to obtain the rooting material a;

[0027] (2) Insert the base of the seedlings in the rooting material a into the rooting I medium for dark culture, the dark culture time is 36 hours, and the temperature is 24 ° C, to obtain the rooting material b;

[0028] Among them, the rooting I medium is: 1 portion of walnut special medium DKW as the basal medium, supplemented with 8 mg / L indole butyric acid IBA, 50 g / L sucrose and ...

Embodiment 2-6

[0033] Embodiment 2-6 The rooting method in the bottle of strange walnut Ulak tissue culture seedling

[0034] Embodiment 2-6 is respectively a kind of rooting method in the bottle of exotic walnut Ulak tissue culture seedling, and it is similar to embodiment 1, and the only difference is that the technical parameters involved in the method are different, specifically refer to the following table:

[0035]

[0036] Wherein, raw material components and consumption in the rooting I medium and rooting II medium in embodiment 2-6 are shown in the following table:

[0037]

Embodiment 7

[0038] Embodiment 7 Screening of rooting conditions

[0039]One, the screening of dark treatment temperature and time in step (2)

[0040] test group Dark treatment temperature (℃) Dark processing time (h) Rooting rate (%) Greenhouse survival rate (%) 1 15 36 15 8 2 18 42 23 40 3 18 48 62 53 4 20 42 50 67 5 20 48 72 83 6 23 54 70 80 7 25 63 74 85 8 28 72 75 81 9 30 78 68 55 10 28 78 70 58 11 25 72 63 72

[0041] It can be seen from the above table that the best dark treatment temperature is 20-28°C, the dark treatment time is 48-72h, and the rooting rate and greenhouse survival rate reach 70-75% and 80-85% respectively; but when the dark treatment temperature and When the time does not reach the lower limit of the range or exceeds the upper limit of the range, the rooting rate and the survival rate of the greenhouse are low; when the temperature is in the range but the ...

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Abstract

The invention discloses a rooting method of a Juglans hindsii*J.regia vlach tissue culture seedling in a bottle. A healthy Juglans hindsii*J.regia vlach tissue culture seedling in a subculture period is used as a rooting material and is treated by dark culture; then the Juglans hindsii*J.regia vlach tissue culture seedling is conveyed into a rooting culture medium to be subjected to rooting culture with alternative light culture and dark culture; a tissue culture rooting seedling which is planted in the bottle can be obtained after 20-24 days; and the rooting rate is up to 65%-75% and the greenhouse survival rate is up to 65%-85%. The rooting method of the Juglans hindsii*J.regia vlach tissue culture seedling in the bottle disclosed by the invention is not influenced by factors including seasons and the like, and theoretical basis and technical supports are provided for industrialized production, and uniform and stable germchits can be efficiently and rapidly provided; and the popularization speed of improved variety is accelerated. The rooting method is applicable to rooting culture of the Juglans hindsii*J.regia vlach tissue culture seedling in the bottle.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a plant rooting method, in particular to a rooting method in a bottle of exotic walnut Ulak tissue culture seedlings. Background technique [0002] Walnut is a kind of economic forest tree species that is difficult to root, and the rooting characteristics of different walnut varieties are significantly different. According to Gale McGranahan's 2004 report from the University of California in the United States, among the three main walnut cultivars adopted in the laboratory, Sunlan, Chandler, and Vina, the rooting conditions were very different, and the growth The variety with strong vigor and erect tree posture, Shenglante, is the easiest to root, with a rooting rate of 94%; Chantler's rooting ability is at the middle level, reaching 55%; Weiner is the most difficult variety, with a rooting rate of only 27%. This study also confirmed that walnut variety or genotype is the key fact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈丽闽奚声珂林大为张承妹董举文
Owner 上海菁艺生物科技有限公司
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