Medium and propagation method for tissue culture rapid propagation of Begonia chinensis

A technology of tissue culture and culture medium, applied in the field of plant tissue culture, can solve the problems of affecting organ development, many calluses, long production cycle, etc., and achieve the effects of accelerating the promotion speed, increasing the reproduction coefficient, and improving the quality

Inactive Publication Date: 2019-03-29
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the tissue culture of Begonia chinensis, and there are only reports on the tissue culture of other species of Begonia genus Cauliflower, such as Lin Junlie (2009) and Hong Zhen (2015), etc., but these reports all have the following Question: (1) MS is selected as the basic medium, and then different hormone ratios are selected. The value-added multiple of proliferation and growth is generally 6-8, and the production cycle is long; Although the induced differentiation rate is high and more callus is produced, it is prone to vitrification, which inhibits the formation of buds and affects organ development.

Method used

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  • Medium and propagation method for tissue culture rapid propagation of Begonia chinensis
  • Medium and propagation method for tissue culture rapid propagation of Begonia chinensis
  • Medium and propagation method for tissue culture rapid propagation of Begonia chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of method for the rapid propagation of the tissue culture of Chinese wild crabapple, carry out following steps successively:

[0040] (1) The preparation of the culture medium, including the components of the basic medium and the culture medium at each stage of tissue culture, and the weight per liter:

[0041] 1) Basic medium: WPM basic medium is used for aseptic seedling cultivation, adventitious bud induction differentiation, subculture proliferation and strong seedling medium, and 1 / 2WPM is used for rooting culture.

[0042] 2) Aseptic seedling medium: WPM+sugar 20g / L+agar 8g / L, pH5.6~5.8

[0043] 3) Adventitious bud induction medium: WPM + 6-BA 1.0 mg / L+ IBA 0.1mg / L;

[0044] 4) Subculture medium: WPM + 6-BA 1.0 mg / L + IBA 0.1 mg / L + sugar 25g / L + agar 5g / L, pH 5.6-5.8.

[0045] 5) The rooting medium is: 1 / 2WPM + NAA0.5mg / L + white sugar 30g / L + agar 5g / L, pH5.6~5.8;

[0046] (2) Cultivation of Tissue Culture Seedlings of Begonia sinensis

[0047] 1) Sel...

Embodiment 2

[0056] In this example, the preparation of the medium in step (1), including the basic medium and the components of the medium at each stage of tissue culture, and the weight per liter are:

[0057] The culture medium for sterile seedlings is: WPM + white sugar 25g / L + agar 5g / L, pH5.6~5.8;

[0058] Adventitious bud induction medium: WPM + 6-BA 2.0 mg / L+ IBA 0.2mg / L;

[0059] The subculture medium is: WPM+ 6-BA2.0 mg / L + IBA 0.2 mg / L+ white sugar 30g / L+ agar 5g / L, pH5.6~5.8.

[0060] The rooting medium is: 1 / 2WPM + NAA1.0 mg / L + white sugar 30g / L + agar 5g / L, pH5.6~5.8;

[0061] All the other steps, technology are with embodiment 1.

Embodiment 3

[0063] In this example, the preparation of the medium in step (1), including the basic medium and the components of the medium at each stage of tissue culture, and the weight per liter are:

[0064] The sterile seedling medium is: WPM + white sugar 30g / L + agar 5g / L, pH5.6~5.8;

[0065] Adventitious bud induction medium: WPM + 6-BA 3.0 mg / L+ IBA 0.3mg / L;

[0066] The subculture medium is: WPM+ 6-BA3.0 mg / L + IBA0.3 mg / L+ white sugar 30g / L+ agar 5g / L, pH5.6~5.8.

[0067] The rooting medium is: 1 / 2WPM + NAA1.5 mg / L + white sugar 30g / L + agar 5g / L, pH5.6~5.8;

[0068] All the other steps, technology are with embodiment 1.

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Abstract

The invention discloses a culture medium for tissue-culture rapid propagation of Bredia sinensis and a propagation method. A WPM culture medium, which is more applicable to shrubs and woody plants, isadopted as a basic culture medium, and the high-efficiency regeneration of tissue-culture plants of the Bredia sinensis is achieved through continuous subculture and proper dosage of a hormone combination; in addition, aseptic treatment on explants, culture of aseptic seedlings of the explants, and the like are improved. According to the culture medium and the propagation method, the operation issimple, the rate of pollution is low, and the success rate of plant regeneration is high.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium and a propagation method for rapid tissue culture propagation of Begonia chinensis. Background technique [0002] Chinese wild crabapple ( Bredia sinensis ), a plant of the genus Begonia in the family Melastaceae, widely distributed in the mountainous areas of southern Zhejiang, green all year round, full of colorful flowers, beautiful colors, elegant posture, high ornamental value in gardening applications, and is an excellent garden landscaping ornamental tree species, both The leaves and flowers can be seen, and the whole plant of this species can be used for medicine. Begonia chinensis has high economic benefits, extensive development and application, and a promising market prospect. [0003] The conventional propagation method of Begonia chinensis is seed propagation, but the seeds are small, the germination rate is low (Tu Juanli et al., 2003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 邹清成葛亚英马广莹史小华田丹青
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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