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Shellfish jacket membrane collagen and method for making same

A collagen and mantle technology, applied in the field of biological resources and the environment, can solve the problems of waste of resources, environmental pollution, underutilization, etc., and achieve the effect of low cost and increased added value

Active Publication Date: 2008-04-09
SHANGHAI FISHERIES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the production of freshwater pearls in my country increases year by year, the output of mussel waste after pearl mining is also increasing. After pearl mining, about 30,000 tons of mussel waste is produced in my country every year, of which the waste of soft parts reaches about 12,000 tons. Only less than 0.1% of the waste has been developed and utilized. Except for processing into low-value-added feed, it is often discarded and not fully utilized. This not only causes serious pollution to the environment, but also damages resources. A kind of waste that exacerbates the burden of today's pearl industry

Method used

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  • Shellfish jacket membrane collagen and method for making same
  • Shellfish jacket membrane collagen and method for making same
  • Shellfish jacket membrane collagen and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Raw material collection and cleaning

[0033] About 4 to 5 years old, the pearls have been cultivated and matured. After killing the mussels, take out the pearls, collect the mantle of the Trigonaus mussels, and wash them with distilled water.

[0034] 2. Use eluate to remove impurities

[0035] Put the cleaned mantle of Triangula clam into a tissue masher and mash it, and add 10 times of the eluate used in the present invention to homogenize. After being stirred for 30 minutes, the precipitate was collected by centrifugation, and then the eluate was added to repeat the treatment for 1-2 times.

[0036] 3. Use the extract to extract collagen

[0037] Remove the precipitate after removing the impurities, add 10 times of the extract and extract at a low temperature of 5°C for 72 hours, so that the collagen is fully dissolved in the extract, and then centrifuge to take the supernatant to obtain a collagen solution. Add KCl to the solution to adjust its concentration ...

Embodiment 2

[0041] After picking the pearls, the mantle of the triangular clam is cleaned with deionized water, weighed a certain amount, and added 5 times of the eluate used in the present invention to mash and homogenize. After being stirred for 40 minutes, the precipitate was collected by centrifugation, and then 5 times of eluate was added to repeat the treatment twice. Add 10 times the extracted liquid to the centrifuged precipitate, extract at 10°C for 48 hours, centrifuge to get the supernatant; add NaCl to the supernatant to make the final concentration reach 12%, then centrifuge to get the precipitate, and dialyze And dry to obtain white, flocculent, no fishy smell Collagen product of the mantle of Triangle sail mussel. The α-peptide chain molecular weight of the product is 430KD, and the purity is about 90%.

Embodiment 3

[0043] After the pearling, the raw material of the triangular sail mussel is cleaned with distilled water, put into its tissue masher, add 2 times of the eluate for homogenization, then add 8 times of the eluate for the raw material, and continue to homogenize. The raw material and the eluate fully interact, the supernatant is removed by centrifugation, and then the eluate 10 times that of the raw material is added, and the treatment is repeated. Centrifuge to remove the supernatant, add 15 times the extract to the precipitate, extract at 5°C for 60 hours, centrifuge to remove the precipitate, add KCl to the supernatant to make the final concentration reach 15%, then centrifuge to take the precipitate, and dialyze and dry to obtain a collagen product. The product is white, flocculent, and has no fishy smell. The molecular weight of the α-peptide chain is about 430KD, and the purity is greater than 90%.

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Abstract

The present invention pertains to the field of biological resources and environmental technology, in particular to a shellfish mantle collagen product and a preparation method thereof. The present invention discloses a shellfish collagen, the collagen is extracted from the shellfish mantles, which is white, flocculent and has no flavor, the molecular weight of an Alpha peptide chain is about 430KD, which is different form the collagens with the sources of terrestrial animals (such as, cows, pigs, rats) and the already reported fish type I collagens, the molecular weights of the Alpha peptide chain of the collagens are all around 300KD (specifically as the attached figures). The shellfish mantles, such as, freshwater pearl mussel mantles, pertain to the by-products after the pearl collection and are discarded usually. The rich amount of collagens in the raw materials can be recovered and utilized by the present invention, so as to change the waste to the valuable matters, which can not only improve the additional value of the products, but also mitigate the pollution level of the environment. The extraction method of the shellfish mantle collagen which is disclosed by the present invention uses the common reagents and simple physical method, the cost is low, the method is safe and non-toxic, so the present invention is applicable to the industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological resources and environment, and in particular relates to a shellfish mantle collagen product and a preparation method thereof. Background technique [0002] Collagen is the protein with the most content in animals, especially mammals. It is widely found in animal skin, bone, cartilage, teeth, tendons, ligaments and blood vessels. It accounts for 30% of the total protein in the human body. It is an extremely important connective tissue. The protein structure plays the role of supporting organs and protecting the body. At present, collagen has been widely used in cosmetics, artificial skin, tissue engineering and other biomedical materials and food health care. Collagen in industrial production is mainly derived from cowhide, pigskin and bones. Due to the occurrence of infectious diseases such as mad cow disease and foot-and-mouth disease, the use of cowhide and pig skin to extract collagen has ...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14
Inventor 刘承初周海燕王金余李应森李家乐
Owner SHANGHAI FISHERIES UNIV
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