Tumour angiogenesis external co-culture model

Abstract

The invention relates to a co-culture model of microencapsulated tumor cells and endothelial cells. The preparation method comprises the steps: first, tumor cells are mixed into 2 percent of sodium alginate solution to lead cells suspension which is injected with 100mmol/L of calcium chloride solution to be colloidized through a microcapsule generator then; normal saline is used for washing the obtained material which then reacts with 0.1 percent of polylysine solution and is washed by normal saline; next, 0.15 percent of sodium alginate solution is added and washed by normal saline; the 55mmol/L sodium citrate solution is employed to liquefy microcapsule core and cultured in a CO₂ of culture box; the microencapsulated tumor cells are directly used for being cultured or frozen reserved; the endothelial cells are employed to be adherently cultured or led to three dimensional growth; the microencapsulated tumor cells are added into the same culture solution; co-culture is done; tumor cells and endothelial cells are separated; finally, travelling gene detection, protein detection and culture liquid cytokine detection are done. The invention overcomes the limitation of Matrigel method and the defect of Transwell method, has an imitated micro-environment for microencapsulated tumor cells and endothelial cells to interact, and has long service life for one-time making and decreases research personnel and financial resources.

Description

technical field [0001] The invention relates to tumor angiogenesis, in particular to a co-cultivation model of microencapsulated tumor cells and endothelial cells. Background technique [0002] In the early stage of tumor growth, after the solid tumor grows to 1 to 2 mm, its continued growth will depend on the formation of new blood vessels. Prior to this, tumor cells themselves have released a large number of pro-angiogenic factors to induce tumor angiogenesis. These new blood vessels are the basis for material exchange between the tumor and the outside world, and angiogenesis is closely related to the development, invasion and metastasis of malignant tumors. Therefore, it is very necessary and urgent to study the mechanism of angiogenesis, especially the early tumor angiogenesis. [0003] Establishing tumor angiogenesis model is an important means to study its formation mechanism. Among them, the in vitro co-culture model is an effective method to simulate tumor angioge...

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Problems solved by technology

Cells inside and outside the Transwell chamber are easy to separate, but Transwell is expensive and has not been ab

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