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Tissue cultivation quick breeding method of orchid

A tissue culture, rapid technology, applied in the field of plant tissue culture, can solve the problems of small reproduction coefficient, limited to individual species in tissue culture, and can not meet the market consumption demand, and achieve the effect of avoiding tissue browning and superior tissue growth conditions

Inactive Publication Date: 2008-05-21
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional orchid propagation method mostly adopts division propagation method, the reproduction coefficient is small, the degree of industrialization is low, and it is far from meeting the market consumption demand
Although there are some research reports on tissue culture of Chinese orchids, the success of tissue culture is limited to individual species, and the growth of protocorms is easy to brown, so the research progress is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, Chunlan young bud initial protocorm induction and proliferation culture:

[0014] Protocorm induction stage:

[0015] Choose Chunlan plants with healthy and disease-free young shoots to be pre-prevented or sterilized for 1 to 2 weeks, rinse them with tap water, cut off the young shoots with a sterile scalpel, wipe them with 75% alcohol or place them in 75% alcohol Soak it in water for about 1min, take it out and rinse it once with sterile water, and then soak it in 0.1% HgCl 2 Soak in medium for 15 minutes, take it out and wash it with sterile water for 2-3 times, blot the water with sterile filter paper, peel off the outer leaves of the young shoots, take the stem tips and put them in a semi-solid medium mixed with improved MS basic medium and additional components Protocorm induction medium, the composition and content of the induction medium are: KNO 3 1200~1500mg / L, NH 4 NO 3 1000~1350mg / L, KH 2 PO 4 110~140mg / L, MgSO 4 ·7H 2 O230~300mg / L, CaCl...

Embodiment 2

[0018] Embodiment 2, Chunlan seed initial protocorm induction and proliferation culture

[0019] Protocorm induction stage:

[0020] Select the spring orchid seed pods with normal development and disease-free and cut them off when they are 8-9 mature, put them in a low-temperature refrigerator (4°C) for 2-3 days, rinse them with tap water first, then scrub them with 75% alcohol, soak them in 0.1% HgCl 2 After 20 minutes, take it out and rinse it with sterile water for 2-3 times, wash dry with sterile filter paper, dissect the seed pods with a sterile scalpel, take a few seeds and inoculate them in the raw medium mixed with semi-solid modified MS basic medium and additional components. Corm induction medium, the composition and content of the induction medium are: KNO 3 1200~1500mg / L, NH 4 NO 3 1000~1350mg / L, KH 2 PO 4 110~140mg / L, MgSO 4 ·7H 2 O230~300mg / L, CaCl 2 200~280mg / L, KI0.83mg / L, H 3 BO 3 6.2mg / L, MnSO4 4H 2 O22.3mg / L, ZnSO4 7H 2 O8.6mg / L, Na 2 MoO 4 2H ...

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PUM

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Abstract

The invention discloses a fast propagation method of orchid tissue culture, including culture steps of inducing, reproducing and successive transfer. An inducing medium used for inducing culture is a semi-solid medium taking an improved MS as a basic medium and adding with other ingredients; a medium used for reproducing and successive transfer is a two-phase medium of liquid and solid taking the improved MS as the basic medium and adding with other ingredients. The gentle shaking of a temperature-controlling shaking bed is combined into the invention. The invention realizes the target of highly efficient inducing and reproducing, alleviating browning and effective successive transfer, which is especially suitable for the orchid tissue culture.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of orchid tissue culture, mainly through the orchid buds or seeds on the induction medium to induce initial (like) protocorms, and then through the liquid-solid two-phase proliferation medium in the The internal temperature control and speed control shaking culture in the temperature-controlled shaker achieves the purpose of high-efficiency proliferation. Background technique [0002] Protocorm or protocorm-like is a unique regeneration method of Orchidaceae. Inducing and multiplying protocorm or protocorm-like is an effective way for Orchidaceae to obtain rapid reproduction. For Orchidaceae, its seeds and young shoots can generally induce protocorms or protocorms through tissue culture, which are collectively referred to as (class) protocorms, and then a large number of miniature plants can be formed through differentiation and rooting cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 孙志栋陈惠云
Owner NINGBO ACAD OF AGRI SCI
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