Method for extracting unmodified natural collagen from tanning waste leather scrap

A technology of natural collagen and leftovers, which is applied in the field of extracting undenatured natural collagen, can solve the problems of low added value and no biological activity, and achieve unique biocompatibility, low biodegradability and broad application prospects

Inactive Publication Date: 2010-06-09
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] To sum up, the products extracted from the leather scrap waste produced in tanning are all hydrolyzed collagen, which has no biological activity and low added value

Method used

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  • Method for extracting unmodified natural collagen from tanning waste leather scrap
  • Method for extracting unmodified natural collagen from tanning waste leather scrap
  • Method for extracting unmodified natural collagen from tanning waste leather scrap

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: get the leftovers of beef slices ash and alkali skin, mince, get 100g, adjust pH=8 with 2M NaOH solution to make the skin swell for 24 hours, use concentration of 30g / L solution pH=8 H 2 o 2 Bleach for 3 hours, then decalcify with 200g of 0.5M EDTA decalcification solution for 24 hours, fully elutriate with tap water, filter to obtain sediment, adjust pH=2.5 with acid, add 2g of pepsin, and stir at 4-6°C for 24 hours. The filter residue was removed by centrifugation, 0.7M NaCl was added to the supernatant for salting out, and the precipitate was dissolved with 0.5M acetic acid to obtain about 600 g of a 5% cowhide undenatured natural collagen solution.

Embodiment 2

[0028] Embodiment 2: get the leftovers of beef slice ash and alkali skin, mince, get 100g, adjust pH=12 with 2M NaOH solution and make skin swell for 48 hours, be that 60g / L solution pH=10 H with concentration 2 o 2Bleach for 8 hours, then use 250g of 1.0M HCl decalcification solution to decalcify for 48 hours, fully elutriate with tap water, filter to get sediment, adjust pH=3 with acid, add 537 protease 5g, stir at temperature 4-6°C for 48 hours, The filter residue was removed by centrifugation, 2.1M NaCl was added to the supernatant for salting out, and the precipitate was dissolved with 0.5M acetic acid to obtain about 650 g of undenatured natural collagen solution of cowhide with a concentration of 8%.

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Abstract

Provided is a method for extracting unmodified natural collagen from leftover materials of tanning scrap leather, which is characterized in that according to part by weight, 100 parts leftover materials of grey cow slice alkali skin are regulated to be with PH value of 8 to 12 by using alkali, which enables the skin to swell for 24 to 48 hours, and the leftover materials are bleached for 3 to 8 hours by H2O2 with PH value of 8 to 12 and the solution concentration of 15 to 60 grams per Liter, and is further delimed, elutriated and filtered to obtain sediment. The 100 parts of the sediment is added with 0.5 to 6 parts of protease, regulated with PH value of 2 to 4 by acid, agitated at temperature of 4 to 6 DEG C for 1 to 3 days, centrifugalized to obtain collagen solution and salted out by 0.5 to 3 MNaCl, and the sediment after being centrifugalized is repeatedly processed for 1 to 3 times by employing 0.05 to 2 M etic acid to dissolve the sediment to be finally dialyzed via films, thereby obtaining the collagen solution of 5 to 8 percent unmodified natural cow skin.

Description

[0001] This case is applied for by Chinese patent 200610022188.5 technical field [0002] The invention relates to a method for extracting undenatured natural collagen from tanning waste leather leftovers, belonging to the field of high value-added transformation of tanning solid waste. Background technique [0003] According to incomplete statistics, my country processes 170 million pieces of leather such as pigs and cattle every year, and produces about 700,000 tons of scrap waste (without chromium). Among them, it is mainly raw leather trimming scraps, scraps of gray leather or scraps produced by tanned leather. These solid wastes are one of the important factors causing serious pollution in the leather industry. Since their main component is collagen, using leather leftovers to extract high-quality collagen and develop it into high-value-added products can not only provide a way for the leather industry to solve solid waste pollution, but more importantly, animal matter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09H3/00C12S3/16
Inventor 李国英李东赵帅李洋
Owner SICHUAN UNIV
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