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Cell vaccine for preventing and controlling hepar damnification

A technology of liver injury and cell population, which is applied in the fields of biotechnology and medicine, and can solve problems such as the preparation of vaccines in hepatitis models

Inactive Publication Date: 2008-06-11
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for preparing vaccines in the art using the concanavalin A-induced hepatitis model itself

Method used

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  • Cell vaccine for preventing and controlling hepar damnification
  • Cell vaccine for preventing and controlling hepar damnification
  • Cell vaccine for preventing and controlling hepar damnification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 lymphocyte vaccine

[0083] Prepare CIH model:

[0084] Concanavalin A (15 mg / kg body weight, purchased from Sigma Company) or PBS was injected into the tail vein of C57BL / 6 mice (purchased from Shanghai Slack Experimental Animal Co., Ltd.) to induce CIH model.

[0085] Preparation of Vaccine 1:

[0086] After inducing the CIH model for 17 hours, the spleen of the mouse was taken out, placed in PBS, and the spleen was ground through a nylon mesh to obtain a cell suspension, and red blood cell lysate was added to remove red blood cells to obtain a mononuclear cell suspension, and the concentration of the cells was adjusted. 5×10 6 / 200μl and 1×10 7 / 200μl, irradiated 4000rad as spleen-derived lymphocyte vaccine (CV).

[0087] Preparation of Vaccine 2:

[0088] 17 hours after the induction of the CIH model, before taking out the mouse spleen, the peripheral blood of the mouse was obtained by collecting blood from the heart, anticoagula...

Embodiment 2

[0089]Example 2 Cell vaccine can reduce the level of alanine aminotransferase (ALT) in plasma

[0090] The cellular vaccine was prepared according to the method described in Example 1, and the C57BL / 6 mice were divided into three groups, four in each group:

[0091] Control group: inject PBS;

[0092] Pathogenic lymphocyte treatment group: injection of CV;

[0093] Non-sensitized lymphocyte treatment group: inject the lymphocytes isolated from the spleen of mice that did not induce CIH ( CV).

[0094] Mice received PBS, PBS, and CV, and CV. That is: the mice in the control group only received the injection of PBS; Mice in the CV group received irradiated spleen lymphocytes (1×10 7 cells / mouse); mice in the CV group received irradiated spleen lymphocytes (1×10 7 cells / mouse).

[0095] On day 14, all mice received a tail vein injection of concanavalin A at 15 mg / kg body weight. After 17-20 hours, the plasma of the mouse was taken out to measure the level of ALT (the ...

Embodiment 3

[0097] Example 3 Cellular Vaccine Can Reduce Liver Damage

[0098] The cellular vaccine was prepared according to the method described in Example 1, and the C57BL / 6 mice were divided into three groups, four in each group:

[0099] Control group: inject PBS;

[0100] Pathogenic lymphocyte treatment group: injection of CV;

[0101] Non-sensitized lymphocyte treatment group: injection cv.

[0102] Mice received PBS, PBS, and CV, and CV. That is: the mice in the control group only received the injection of PBS; Mice in the CV group received irradiated spleen lymphocytes (1×10 7 cells / mouse); mice in the CV group received irradiated spleen lymphocytes from CIH mice.

[0103] On day 14, all mice were given a tail vein injection of concanavalin A at 15 mg / kg. After 17-20 hours, the livers of the mice were removed and fixed with 4% paraformaldehyde. Tissue sections of the liver were subjected to routine H&E staining and TUNNEL staining, and were examined by light microscop...

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Abstract

The invention discloses a cell group, the cell group originates from a model animal induced by canavalin A, wherein, more than 90 percent adopts mononuclear cells, and the cell group can obviously reduce the level of glutamate pyruvate transaminase in the body after being injected in a mammal body. The invention also discloses a preparation method of the cell group and combination including the cell group. The effect of the cell group obtained by the invention of preventing the damage of a liver has great superiority over other medicinal; and the adoption of the method of the invention can obtain a great quantity of mononuclear cells, and the preparation is simple, the obtained quantity is large, the culture in vitro is not required, thereby, the invention can be used for preparing the cell vaccine, and overcome the defect that the enough immunological cells are usually difficult to be obtained from a pathogenesis individual body by the field, and the pertinent cells are difficult to be obtained.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, and more specifically, the invention relates to a cell group for preventing and treating liver damage, its preparation method, application, and a vaccine prepared by using the cell group. Background technique [0002] Since the 20th century, the incidence of hepatitis has gradually increased and has become one of the diseases that seriously threaten human health. From the perspective of etiology, hepatitis mainly includes viral hepatitis, alcoholic hepatitis, autoimmune hepatitis and so on. [0003] In China, patients with various types of hepatitis are on the rise, and nearly 400,000 people die of liver disease every year. Hepatitis infections are distributed all over the world. From the perspective of the world's population, 6 billion people, 2 billion of them have ever been infected with hepatitis B (HBV), accounting for one-third of the global population. Among the 2 billion people...

Claims

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Application Information

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IPC IPC(8): C12N5/06A61K35/12A61P1/16C12N5/0786
Inventor 徐凌云梅云华王莹
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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