Nitrile hydratase industrial fermentation method

Abstract

The present invention provides a nitrile hydratase industrial fermentation production prescription and a fermentation method. The prior nitrile hydratase industrial fermentation base fluid is used asa base, trisodium phosphate is added into the fermentation base fluid and the adding quantity is 0.13 percent to 0.15 percent of the weight of fermentation fluid. The prescription is adopted, after material is put and dissolved, sodium hydroxide is used to adjust the pH value to be 7 to 8; then disinfection is processed; when the temperature rises till 110 DEG C to 120 DEG C, the pressure is keptfor 10 to 20 minutes; subsequently, the temperature of the material in a fermentation tank is reduced to 20 DEG C to 30 DEG C fast; when a strain meets the index, the subcultivation is processed for the fermentation production. The method can improve the activity of the nitrile hydratase by more than 50 percent and synchronously can ensure that the fermentation production period of the fermentation tank is shortened by one third, which does not require adding new devices and is simple for carrying out; compared with the feeding methods of feeding sugar, feeding sodium hydroxide, etc., the method of the present invention reduces additional work and the operation and control difficulty brought by feeding during the course of the fermentation and eliminates the occurrence of the status that the fermentation tank is infected bacteria caused by feeding.

Description

technical field [0001] The invention relates to a method for industrial fermentation of nitrile hydratase. Background technique [0002] Nitrile hydratase production cells are affected by the slow utilization of glucose by the cells in the early stage of fermentation. On the one hand, the fermentation production cycle is too long, and the general fermentation production cycle is 66 hours. On the other hand, the cells The growth period is relatively long and the production period is relatively short, which affects the enzyme production and enzyme activity of the bacteria. Generally, the enzyme activity produced by fermentation is about 6 million uAAM / ml.h. What is usually adopted is the method of adding supplementary materials such as glucose and sodium hydroxide during the fermentation process to regulate the metabolic process of the bacteria. [0003] JP8173152 and US2005239165 disclose adding inorganic salt as a buffer in the nitrilase fermentation production formula, and...

AI Technical Summary

Problems solved by technology

[0004] Although it has a certain effect, it is cumbersome to implement, and it is even necessary to add new equipment and facilities, and the operation is difficult. While increasing the investment in fixed assets, the fermenter is often contaminated by bacteria due to feeding