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Demanganization functional bacterium

A technology of functional bacteria and Pseudomonas, applied in the field of microorganisms, can solve the problems of long recovery period of engineering flora, poor manganese removal effect, long startup time, etc., so as to shorten the establishment time, speed up the filtration speed, and shorten the recovery period. Effect

Inactive Publication Date: 2008-07-09
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that the current bio-oxidative manganese removal engineering bacteria group is established by natural selection, the biochemical ability is weak, the manganese removal effect is poor, and the filtration speed is slow; Changhe Engineering Bacteria has a long recovery period after being destroyed, and provides a manganese-removing functional bacterium

Method used

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  • Demanganization functional bacterium
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specific Embodiment approach 1

[0017] Specific embodiment 1: In this embodiment, the manganese-removing functional bacterium MB32 (Pseudomonas sp.) belongs to the genus Pseudomonas, and is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. The preservation date is October 30, 2007, and the preservation number is It is CGMCC No.2238; the manganese-removing functional bacterium MB32 (Pseudomonas sp.) is a Gram-negative facultative anaerobic bacterium, straight or curved, short rod-shaped, 1-3 μm long, 1-1.5 μm wide, with two cells The end is round and blunt (as shown in Figure 1 and Figure 2); milky white colonies are formed on the culture medium, opaque, with irregular edges, and slightly blue around; the manganese-removing functional bacteria MB32 (Pseudomonassp.) has energy storage particles, spores and pods Membrane, N, N, N', N'-tetramethyl-p-phenylenediamine (N, N, N', N'-Tetramethyl-p-Phenylenedia, TMPD) staining was positive.

[0018] Mangane...

specific Embodiment approach 2

[0020] Specific embodiment 2: In this embodiment, the manganese-removing functional bacteria MB32 (Pseudomonas sp.) is screened from mud taken from the aeration tank wall of the biological manganese removal filter of Zhangshi biological manganese removal water plant in Shenyang City, Liaoning Province. 1L medium for screening consists of 0.8g peptone, 0.2g yeast extract, 0.1g MnSO 4 .H 2 O, 0.1 g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaNO 3 , 0.1g CaCl 2 , 0.1g (NH 4 ) 2 CO 3 , 0.01g FeSO 4 ·7H 2 O and the remainder of distilled water; then sterilized.

[0021] The present embodiment adopts the combination of plate streaking and plate coating method to divide bacteria, and adopts TMPD method (Vandenabeele J, D de Beer et al. Manganese oxidation by microbial consortia from sand filters. Microb. Ecol. 1992, 24: 91-108. ) for the primary screening of the isolated strains. The principle of using the TMPD method in this embodiment is that N, N, N', N'-tetramethyl-p-phe...

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Abstract

A biological demanganization function strain is disclosed, in particular to a microbe. The invention solves the establishment of the prior biological oxidation demanganization function strain which depends on natural selection, weak biochemical capability and bad demanganization effect and long cultured, tamed, and starting time of the water disposal demanganization biological filter tank establishment and long restoration period after the function strain is broken. The biological demanganization function strain MB32 (Pseudomonas sp.) belongs to the pseudomonas and is preserved in the Chinese microbe bacterial preservation management committee common microbe center and the preservation date is Oct. 30, 2007 and the preservation number is CGMCC No.2238. The bacterial MB32 is Gram-negative facultative bacteria which is straight or bent dagger shape and is 1-3 mum in length, 1-1.5 mum in breadth. Two ends of the cell are blunt circle and form lacte colony on the culture medium without transparent and the peripheral color is a little blue. The entire colony is round shape. The strain MB32 has power storage particle, spore and capsule. The dyeing of N, N, N', N'-tetramethyl p-phenylene diamine is positive. The biological demanganization function strain MB32 of the invention is provided with Mn 2+ removing ability and the Mn 2+ clearance is over 92%.

Description

technical field [0001] The present invention relates to a microorganism. Background technique [0002] Manganese is an abundant metal element in the earth's crust. The concentration of manganese ions in ordinary water is 0.1-1 mg / L, while the concentration of soluble manganese ions in anaerobic groundwater can be as high as 7-9 mg / L. Soluble manganese can form insoluble precipitates (such as MnO 2 or MnOOH). [0003] MnO 2 is a highly insoluble substance (δ-MnO 2 The solubility kso is only 10 -56 ), which is dark black and can cause significant coloration in groundwater intended for drinking; manganese must therefore be removed from drinking water. my country stipulates that the maximum concentration of manganese ions in drinking water is 0.1mg / L, while the European Union has determined the maximum concentration of manganese ions in drinking water as 0.05mg / L. [0004] The removal of soluble Mn(II) from groundwater is usually accompanied by oxidation of manganese ions,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/20
Inventor 张杰赵焱李冬
Owner HARBIN INST OF TECH